Oral exposure of Kunming mice to diisononyl phthalate induces hepatic and renal tissue injury through the accumulation of ROS. Protective effect of melatonin
作者：
Ma, Ping;Yan, Biao;Zeng, Qiang;Liu, Xudong;Wu, Yang;...
期刊：
Food and Chemical Toxicology ,2014年68:247-256 ISSN：0278-6915
通讯作者：
Wu, Jiliang
作者机构：
[Wu, Yang; Liu, Chao; Wu, Jiliang; Jiao, Ming; Ma, Ping] Hubei Univ Sci & Technol, Hubei Prov Key Lab Cardiovasc Cerebrovasc & Metab, Xianning 437100, Peoples R China.;[Wu, Yang; Yang, Xu; Liu, Xudong; Yan, Biao; Ma, Ping; Zeng, Qiang] Cent China Normal Univ, Hubei Prov Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wu, Jiliang] H;Hubei Univ Sci & Technol, Hubei Prov Key Lab Cardiovasc Cerebrovasc & Metab, Xianning 437100, Peoples R China.
关键词：
Diisononyl phthalate;Interleukin-1;Melatonin;Oxidative stress;Reactive oxygen species;Tumour necrosis factor alpha
摘要：
Diisononyl phthalate (DINP) has been widely used in polyvinyl chloride (PVC) products and is ubiquitous as a substitute; however, its toxicity due to exposure remains to be determined. This study investigated the oxidative damage induced by DINP and the induced production of the pro-inflammation cytokines interleukin-1 (IL-1) and tumour necrosis factor-α (TNF-α). Oral exposure to DINP induced oxidative damage and inflammatory responses in liver and kidney tissues through the accumulation of ROS, which may be an underlying mechanism for its toxicity. These changes may contribute to hepatic and renal histopathological alterations. Our data suggest that oxidative stress is involved in DINP-induced toxicity and that the co-administration of melatonin exerts a protective effect against DINP-induced toxicity. © 2014 The Authors.
语种：
英文
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北京与武汉部分儿童家庭室内空气中甲醛及挥发性有机物调查
作者：
方建龙;杨旭;李红;董小艳;李韵谱;...
期刊：
环境与健康杂志 ,2014年31(7):585-586 ISSN：1001-5914
作者机构：
中国疾病预防控制中心环境与健康相关产品安全所,北京100021;华中师范大学生命科学学院;[方建龙; 李韵谱; 徐东群; 唐志刚; 董小艳; 李红] 中国疾病预防控制中心;[杨旭] 华中师范大学
关键词：
儿童;空气污染;室内;甲醛;挥发性有机化合物
摘要：
目的 了解北京和武汉春季儿童家庭室内空气中甲醛及挥发性有机物的污染水平.方法 于2009年3-5月分别从北京和武汉各选取一家医院,采用随机数字的方法选取48户和78户儿童家庭作为研究对象,检测室内空气中甲醛、苯、甲苯、二甲苯和总挥发性有机物(TVOC)的浓度.结果 武汉儿童家庭室内空气中甲醛浓度高于北京,超标率分别为88.46％和4.17％;北京室内空气中甲苯、二甲苯和TVOC浓度高于武汉,差异均有统计学意义(P＜0.05).结论 本次调查的武汉儿童家庭室内空气甲醛污染比较严重.
语种：
中文
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Benzyl butyl phthalate exposure impairs learning and memory and attenuates neurotransmission and CREB phosphorylation in mice
作者：
Min, Anna;Liu, Fengming;Yang, Xu;Chen, Mingqing*
期刊：
Food and Chemical Toxicology ,2014年71:81-89 ISSN：0278-6915
通讯作者：
Chen, Mingqing
作者机构：
[Min, Anna; Chen, Mingqing; Liu, Fengming; Yang, Xu] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Chen, Mingqing] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Benzyl butyl phthalate (BBP);CREB;Learning and memory;Neurotoxicity;Neurotransmitter
摘要：
The existing data are insufficient to evaluate the neurotoxicity of benzyl butyl phthalate (BBP) exposure on the brain, and the underlying mechanisms to explain these effects remain unclear. Kun Ming (KM) mice were exposed to BBP (0, 50, 250, 1250. mg/kg) via gavage for 14. days. Employing the Morris water maze (MWM) test, we observed that mice demonstrated poorer learning and memory performance than the control group at doses of 250 and 1250. mg/kg/day. Reduction of locomotor activity in mice and depression were indicated by a long "dead time" in the Forced Swim Test and tail suspension test. Pathological alterations and oxidative damage to the hippocampus were found. To study the molecular mechanisms underlying BBP toxicity, the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) and pCREB were tested. The modified levels of 5-HT in the hippocampus and the decreased levels of CREB phosphorylation after BBP exposure suggested a potential mechanism underlying BBP toxicity. We hypothesize that BBP exposure causes a decrease in the number of neurotransmitters, which in turn down regulates the levels of CREB phosphorylation by the cAMP/protein kinase A (PKA)-mediated signaling. The results are an attenuation of the effects of CREB downstream, oxidative damage and impaired behavioral performance. © 2014 Elsevier Ltd.
语种：
英文
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Influence of BBP exposure on nervous system and antioxidant system in zebrafish
作者：
Zhang, Can;Yang, Xue;He, Zhen;Zhong, Qiu;Guo, Jia;...
期刊：
Ecotoxicology ,2014年23(10):1854-1857 ISSN：0963-9292
通讯作者：
Xiong, Li
作者机构：
[Zhong, Qiu; Xiong, Li; He, Zhen; Liu, Deli; Yang, Xue; Zhang, Can; Hu, Xiao-jing; Guo, Jia] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Xiong, Li] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Butyl benzylphthalate;Zebrafish;AChE;SOD;Semi-quantitative RT-PCR
摘要：
In order to observe the toxic effects of butyl benzyl phthalate (BBP) on zebrafish, the AChE and SOD activity of zebrafish exposed to different concentrations of BBP (0, 0.332, 0.665, 1.33 mg L −1 ) in a short-term (7d) test were determined. Semi-quantitative PCR was used to determine the mRNA transcript levels of the AChE and SOD gene in zebrafish brain and muscle. The results showed: AChE activity decreased with increased exposure concentration, and was significantly inhibited (p < 0.01) compared with the control group at 0.665 mg L −1 concentration. Low BBP concentrations stimulated and high concentrations inhibited SOD activity with a concentration of 0.332 mg L −1 resulting in a significant induction (p < 0.05) compared with the control, and 0.665 and 1.33 mg L −1 concentrations resulting in significant inhibition (p < 0.05, p < 0.01) relative to the control group. The RT-PCR data showed a decrease in brain and muscle mRNA transcription of AChE gene with an increase in exposure concentration. The mRNA transcription of SOD in the brain was not different between the exposed groups and control group; in muscle, the mRNA transcription inhibition decreased and then increased: all differences from the control were statistically significant. © 2014, Springer Science+Business Media New York.
语种：
英文
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单壁碳纳米管对小鼠N2a神经瘤细胞潜在毒性的探讨
作者：
彭梅;于悦;刘旭东;晏彪;付超;...
期刊：
公共卫生与预防医学 ,2014年25(1):11-14 ISSN：1006-2483
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,环境分子生物学研究组,武汉430079
关键词：
单壁碳纳米管;小鼠成神经瘤细胞;细胞活力;氧化损伤
摘要：
目的 探究单壁碳纳米管(single-walled carbon nanotubes,SWCNTs)对小鼠成神经瘤细胞(N2a)的细胞毒性作用.方法 采用不同浓度的SWCNTs悬液(0、12.5、25、50 μg·ml-1)对N2a细胞进行24 h染毒处理后,检测细胞活力,细胞活性氧(reactive oxygen species,ROS)、还原型谷胱甘肽(GSH)水平.结果 随着N2a染毒浓度的增加,N2a细胞活力下降,ROS含量增加,GSH含量下降.在维生素C(Vit C)存在的情况下,最高浓度组(50 μg·ml-1)产生的细胞损伤最大程度上得到缓解.结论 SWCNTs的暴露在某种程度上可对N2a细胞产生一定的细胞毒性,并且VitC可以通过降低氧化损伤对N2a细胞起到一定的保护作用.
语种：
中文
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邻苯二甲酸二丁酯皮肤和经口摄入暴露诱发的小鼠肺损伤
作者：
李俐;李金泉;问华肖;杨旭
期刊：
环境与健康杂志 ,2014年31(2):95-99 ISSN：1001-5914
作者机构：
[李俐; 李金泉; 问华肖; 杨旭] 华中师范大学生命科学学院
关键词：
邻苯二甲酸二丁酯;皮肤暴露;经口暴露;免疫球蛋白E;过敏性哮喘
摘要：
目的探讨邻苯二甲酸二丁酯(DBP)暴露诱发的小鼠肺部损伤。方法采用皮肤和经口摄入两种暴露方式染毒,在每一种暴露方式中,各选32只雄性Balb/C小鼠随机分为4组,每组8只。皮肤暴露的DBP剂量分别为0、4、40和400 mg/kg,经口摄入(灌胃)的DBP剂量分别为0、0.4、4和40 mg/kg,染毒60 d后,对小鼠肺泡灌洗液中白细胞总数及分类进行计数,采用酶联免疫吸附(ELISA)法测定小鼠血清中免疫球蛋白E(IgE)的含量。同时,采用HE染色法观察小鼠肺部气道的病理学变化。最后,通过动物肺功能分析系统检测小鼠气道高反应性的变化。结果两种染毒方式的小鼠肺泡灌洗液中白细胞数、淋巴细胞、嗜酸性粒细胞和血清中IgE均随DBP染毒剂量的升高呈上升趋势,且与对照组相比,皮肤暴露组中DBP染毒剂量≥40 mg/kg,经口暴露组DBP染毒剂量≥4 mg/kg时,差异有统计学意义(P<0.05,P<0.01)。同时,在两种染毒方式中,随着DBP染毒剂量的升高,小鼠肺部气道出现明显病理学变化,但气道高反应性并无明显的改变。结论高剂量DBP暴露(皮肤≥40 mg/kg,经口≥4 mg/kg)可以提高小鼠肺泡灌洗液中白细胞、淋巴细胞、嗜酸性粒细胞和血清中IgE的水平,并且引起明显的气道病理学变化。
语种：
中文
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提高研究水平的策略：环境相关疾病导向性分子毒理学研究
作者：
杨旭
期刊：
公共卫生与预防医学 ,2014年25(1):1-3 ISSN：1006-2483
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,环境生物医学学组,武汉430079
关键词：
环境毒理学;研究水平;研究策略
摘要：
提高所在科研团队SCI论文的影响因子和他引次数,是从事自然科学研究同仁的共同愿望。近5年来本实验室采用了一种新的毒理学研究策略———"环境相关疾病导向性分子毒理学研究",使课题组SCI论文的数量和影响因子迅速提升。为了与毒理学界同仁分享心得,受《公共卫生与预防医学》杂志社的邀请,特撰写本短文。
语种：
中文
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邻苯二甲酸单丁酯致小鼠肝脏和肾脏组织的氧化损伤
作者：
刘志敏;熊棣;曹凤华;梅宇飞;吴卓;...
期刊：
化学与生物工程 ,2014年(8):18-23 ISSN：1672-5425
作者机构：
华中师范大学生命科学学院 遗传调控与整合生物学湖北省重点实验室,湖北 武汉,430079;[袁均林; 杨旭; 梅宇飞; 吴卓; 曹凤华; 熊棣; 刘志敏] 华中师范大学
关键词：
邻苯二甲酸单丁酯;小鼠;肝;肾;氧化损伤
摘要：
为了探究邻苯二甲酸单丁酯（MBP）对小鼠肝脏和肾脏的氧化损伤，将42只 BALB/c 小鼠随机分为7组，每组6只，分别为25 mg·kg^-1、50 mg·kg^-1、100 mg·kg^-1、200 mg·kg^-1的4个 MBP 染毒组、1个100 mg·kg^-1的邻苯二甲酸二丁酯（DBP）染毒组、1个空白对照组、1个溶剂对照组。染毒期间对小鼠的体征进行观察；14 d 后取其肝脏和肾脏组织，制作小鼠肝脏和肾脏组织的切片，对肝脏和肾脏的组织学形态进行观察；制作组织匀浆液用于检测肝脏和肾脏组织细胞的 ROS、GSH、MDA 的含量，以了解 MBP 对肝脏和肾脏组织的氧化损伤作用。结果显示：各剂量组小鼠肝细胞和肾小管上皮细胞均出现不同程度的细胞核固缩、细胞水肿、空泡样变、脂肪滴增大融合等症状；ROS 和 MDA 的含量与 MBP 的染毒剂量呈正相关，GSH 的含量与 MBP 的染毒剂量呈负相关；相同剂量的 DBP 与 MBP 染毒组相比，MBP染毒组的 ROS 和 MDA 含量较高、GSH 含量较低。表明，MBP 的暴露与小鼠肝脏和肾脏组织的氧化损伤存在直接联系。
语种：
中文
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Primary neuronal-astrocytic co-culture platform for neurotoxicity assessment of di-(2-ethylhexyl) phthalate
作者：
Wu, Yang;Li, Ke;Zuo, Haoxiao;Yuan, Ye;Sun, Yi;...
期刊：
环境科学学报(英文版) ,2014年26(5):1145-1153 ISSN：1001-0742
通讯作者：
Yang, Xu
作者机构：
[Wu, Yang; Yuan, Ye; Zuo, Haoxiao; Yang, Xu] Cent China Normal Univ, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Wu, Yang; Sun, Yi] Univ Calif Los Angeles, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA.;[Li, Ke] Wuhan Inst Drug & Food Control, Wuhan 430012, Peoples R China.
通讯机构：
[Yang, Xu] C;Cent China Normal Univ, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
di-(2-ethylhexyl)phthalate;neurotoxicity;immunostaining;oxidative stress;neuron count;neurite length;astrocyte gliosis
摘要：
Plastics such as polyvinyl chlorides (PVC) are widely used in many indoor constructed environments; however, their unbound chemicals, such as di-(2-ethylhexyl) phthalates (DEHP), can leach into the surrounding environment. This study focused on DEHP's effect on the central nervous system by determining the precise DEHP content in mice brain tissue after exposure to the chemical, to evaluate the specific exposure range. Primary neuronal-astrocyte co-culture systems were used as in vitro models for chemical hazard identification of DEHP. Oxidative stress was hypothesized as a probable mechanism involved, and therefore the total reactive oxygen species (ROS) concentration was determined as a biomarker of oxidative stress. In addition, NeuriteTracer, a neurite tracing plugin with ImageJ, was used to develop an assay for neurotoxicity to provide quantitative measurements of neurological parameters, such as neuronal number, neuron count and neurite length, all of which could indicate neurotoxic effects. The results showed that with 1 nmol/L DEHP exposure, there was a significant increase in ROS concentrations, indicating that the neuronal-astrocyte cultures were injured due to exposure to DEHP. In response, astrocyte proliferation (gliosis) was initiated, serving as a mechanism to maintain a homeostatic environment for neurons and protect neurons from toxic chemicals. There is a need to assess the cumulative effects of DEHP in animals to evaluate the possible uptake and effects on the human neuronal system from exposure to DEHP in the indoor environment.
语种：
英文
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In vitro study on cytotoxicity and intracellular formaldehyde concentration changes after exposure to formaldehyde and its derivatives
作者：
Ke, Y. J.;Qin, X. D.;Zhang, Y. C.;Li, H.;Li, R.;...
期刊：
Human & Experimental Toxicology ,2014年33(8):822-830 ISSN：0960-3271
通讯作者：
Ding, S. M.
作者机构：
[Qin, X. D.; Li, R.; Yang, X.; Yuan, J. L.; Ding, S. M.; Li, H.; Zhang, Y. C.; Ke, Y. J.] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Ding, S. M.] Cent China Normal Univ, Coll Life Sci, Lab Environm Biomed, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Ding, S. M.] C;Cent China Normal Univ, Coll Life Sci, Lab Environm Biomed, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.
关键词：
Formaldehyde;formaldehyde dehydrogenase;reactive oxygen species;formaldehyde content;real-time qPCR
摘要：
HeLa cells were exposed to formaldehyde and its metabolic derivatives, methanol, formic acid, and acetaldehyde, to investigate that the toxicity of formaldehyde is not caused by the chemical group. After 1 h of treatment with formaldehyde, mitochondrial assays showed that low concentrations (e.g. 10 εmol/L) of formaldehyde promoted growth of the HeLa cells, while higher concentrations (e.g. ≥62.5 εmol/L) inhibited cell growth; while all four chemicals at a concentration of 125 εmol/L affected cell growth, formaldehyde affected the largest. Reactive oxygen species concentration increased with the concentration of the exposure chemical. The endogenous formaldehyde content increased the most in the formaldehyde group, but in other three groups, it did not increase as the exposure concentration increased. Expression of dehydrogenase (formaldehyde dehydrogenase (FDH)) in the formaldehyde (10.40) and methanol (10.60) groups increased significantly compared with the control (1), while it was similar to the control in formic acid (0.90) and acetaldehyde (1.10) groups. Our results suggest that formaldehyde could affect cell activity and even enter cells. Exposure to formaldehyde changes the endogenous formaldehyde concentration in cells within 24 h, and this induces expression of FDH for formaldehyde degradation to maintain the formaldehyde balance. The toxicity of formaldehyde is not caused by the carbon atoms in the aldehyde, hydroxyl, or carboxyl groups. Formaldehyde is hypothesized to be an important signaling molecule in the regulation of cell growth and maintenance of the endogenous formaldehyde level. © The Author(s) 2014.
语种：
英文
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基于高校医院中高血压病管理的策略研究
作者：
张振业;周鄂生;杨旭
期刊：
保健医学研究与实践 ,2014年11(3):93-94+96 ISSN：1673-873X
作者机构：
[张振业; 周鄂生] 华中师范大学医院;华中师范大学生命科学学院;[杨旭] 华中师范大学
关键词：
高校医院;高血压病;管理策略
摘要：
高血压病是最常见的慢性病之一,其致残、致死率均较高。在高校医院里设立高血压病门诊或心血管病专科门诊,是防控高血压病、控制心脑血管疾病（如脑卒中、冠心病等）的重要环节。本文重点介绍了对高血压病人进行系统化管理、对高危人群进行个体化治疗以及针对家庭与个人健康教育的经验体会,这些措施对防控高血压病有着极其重要的意义。
语种：
中文
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氯氰菊酯对小鼠肾组织的氧化损伤
作者：
马萍;张忠杰;焦铭;廖文莉;陈姣娥;...
期刊：
中国环境科学 ,2014年34(11):2958-2963 ISSN：1000-6923
通讯作者：
Wu, Yang
作者机构：
[张忠杰; 焦铭; 廖文莉; 马萍; 武阳; 陈姣娥] College of Basic Medical, Hubei University of Science and Technology, Xianning , China;[杨旭; 马萍; 武阳] Laboratory of Environment Biomedicine, School of Life Science, Central China Normal University, Wuhan , China
通讯机构：
College of Basic Medical, Hubei University of Science and Technology, Xianning, China
关键词：
氯氰菊酯;维生素E;活性氧;还原型谷胱甘肽;丙二醛;8-羟基脱氧鸟苷;DNA-蛋白质交联;氧化损伤
摘要：
以昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯加维生素E组,染毒组按10,20,40mg/kg水平,维生素E的剂量为100mg/kg,灌胃染毒小鼠7d.以肾组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)和8-羟基脱氧鸟苷(8-OHdG)的含量；以肾组织细胞测定 DNA-蛋白质交联(DPC)系数.随着氯氰菊酯染毒剂量的升高,肾组织的ROS、MDA、8-OHdG含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量为20mg/kg 时,MDA 和8-OHdG 含量差异有统计学意义(P<0.05)；染毒剂量为40mg/kg 时, ROS(F=3.7044)、GSH(F=3.4908)、MDA(F=3.5851)、8-OHdG含量(F=11.7934)和DPC系数(F=6.9165)差异均有统计学意义(P<0.05, P<0.01).病理学观察可见20,40mg/kg剂量组小鼠肾小球增生肥大,肾小管上皮细胞水肿,管腔变小.与高剂量染毒组相比较,高剂量染毒加维生素E组肾组织的ROS、MDA含量、8-OHdG含量和DPC系数均有下降,GSH含量上升(P<0.05, P<0.01).高剂量(≥20mg/kg)的氯氰菊酯能造成小鼠肾组织的氧化损伤,维生素E有抗氧化作用.
语种：
中文
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The adjuvant effect induced by di-(2-ethylhexyl) phthalate (DEHP) is mediated through oxidative stress in a mouse model of asthma
作者：
You, Huihui;Chen, Shaohui;Mao, Lin;Li, Bing;Yuan, Ye;...
期刊：
Food and Chemical Toxicology ,2014年71:272-281 ISSN：0278-6915
通讯作者：
Li, Rui
作者机构：
[Yuan, Ye; Li, Rui; You, Huihui; Chen, Shaohui; Yang, Xu; Mao, Lin; Li, Bing] Cent China Normal Univ, Coll Life Sci, Huibei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Li, Rui] Cent China Normal Univ, Coll Life Sci, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Rui] C;Cent China Normal Univ, Coll Life Sci, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.
关键词：
Di-(2-ethylhexyl) phthalate;Mouse asthma model;Oxidative stress
摘要：
Di-(2-ethylhexyl) phthalate, as the most commonly used plasticizer, is considered to be related to the asthma prevalence. There are studies affirming that the DEHP has an adjuvant effect in the pathogenesis of allergy asthma. Oxidative stress is one possible pathway for DEHP-adjuvant effect. Thus, this study explored whether DEHP could induce adjuvant effect in mouse asthma model via oxidative stress pathway. Male BALB/c mice were randomly divided into six groups: (1) saline group, (2) DEHP group, (3) ovalbumin (OVA) group, (4) DEHP. +. OVA group, (5) OVA. +. vitamin E (Vit E) group, (6) DEHP. +. OVA. +. Vit E group. The exposure dose of DEHP was 30. mg/kg body weight (bw)/day. After 18. days of the exposure protocol. Reactive oxygen species (ROS), glutathione (GSH) and malonaldehyde (MDA) levels and biomarkers related to asthma model were measured. Collectively, these data indicated higher ROS and MDA levels and lower GSH contents in DEHP. +. OVA group than that in OVA group, while Vit E, an antioxidant, could restore ROS, MDA and GSH levels to control levels and attenuate the DEHP and/or OVA effects. Our observations suggested that there was a relationship between oxidative stress and the adjuvant effect induced by DEHP in this mouse asthma model. © 2014 The Authors.
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英文
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Anti-bensulfuron methyl monoclonal antibody production and BSM-detecting indirect competitive enzyme-linked immunoassay establishment
作者：
Zhao, Jingyun;Yu, Xiaowei;Luo, Qing;Wei, Chenxi;Ke, Chenjuan;...
期刊：
Food and Agricultural Immunology ,2014年25(3):350-363 ISSN：0954-0105
通讯作者：
Yang, Xu
作者机构：
[Wei, Chenxi; Yuan, Junlin; Zhao, Jingyun; Luo, Qing; Ke, Chenjuan; Zuo, Haoxiao; Yang, Xu; Yu, Xiaowei] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Peoples R China.
通讯机构：
[Yang, Xu] H;Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Peoples R China.
关键词：
anti-BSM monoclonal antibody (anti-BSM MAb);bensulfuron methyl (BSM);food safety;indirect competitive enzyme-linked immunoassay (icELISA)
摘要：
Bensulfuron methyl (BSM) is widely used for agricultural purposes and has raised health concerns, as well as ecological problems. Immunoassay would be one of the most advantaged measurements compared with traditional methods for BSM detection and measurement. In order to develop indirect competitive enzyme-linked immunoassay (icELISA), the anti-BSM monoclonal antibody (anti-BSM MAb) was produced. For the MAb production, BSM was conjugated to OVA and injected to mice with Freud's adjuvant for immunisation. Antiserum screening has revealed successful immunising. One stable hybridoma cell line (2H1) was obtained through cell fusion between spleen cells of immunised mouse and SP 2/0 cells. The MAb secreted by 2H1 cells was of high affinity and sensitivity, as well as specificity to BSM. Then the protocol of the icELISA and standard curve for BSM measurement was made and examined by controlled application. Significantly, the application has exhibited 96.530%-107.2% recovery of BSM. The produced MAb and established immunoassay may facilitate the measurement of BSM and herein help to ensure food safety and regulate environmental protection. © 2013 Taylor & Francis.
语种：
英文
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邻苯二甲酸丁基苄酯致神经细胞氧化损伤
作者：
闵安娜;刘锋明;晏彪;陈明清;杨旭
期刊：
生态毒理学报 ,2014年9(1):97-102 ISSN：1673-5897
作者机构：
[闵安娜; 刘锋明; 晏彪; 陈明清; 杨旭] 华中师范大学生命科学学院
关键词：
邻苯二甲酸丁基苄酯;细胞凋亡;细胞毒性;氧化损伤;神经毒性
摘要：
为探究邻苯二甲酸丁基苄酯(butyl benzyl phthalate,BBP)对小鼠神经的毒性作用,进行了小鼠体外毒理学研究。首先用不同浓度的邻苯二甲酸丁基苄酯染毒神经模型细胞—N_2 a 神经瘤细胞,通过噻唑蓝比色法(MTT) ,Hoechst 33258染色实验评价邻苯二甲酸丁基苄酯的细胞毒效应; 通过对染毒细胞氧自由基(ROS) 、丙二醛(MDA) 、还原型谷胱甘肽(GSH)含量的检测来探究BBP 对小鼠神经瘤细胞的氧化损伤效应。随着BBP 浓度的不断增高,细胞的MTT 值逐渐变小,当BBP 的浓度达到10 g·L~(-1)时,MTT 实验结果与对照组出现显著性差异; Hoechst 33258染色结果显示: 高浓度的BBP 导致细胞核呈现出不规则状态,出现了凋亡小体; 随着BBP 染毒浓度的升高N_2 a 细胞中的ROS 水平和MDA 含量逐渐上升,分别在0. 16 g·L~(-1)和10 g·L~(-1)开始与对照组相比出现了显著性的差异(p < 0.05); 而GSH 系数呈现下降趋势,在0.32 g·L~(-1)时开始出现显著性差异(p < 0.05)。实验结果表明高浓度的邻苯二甲酸丁基苄酯可以导致神经瘤细胞的凋亡,并产生氧化损伤效应。
语种：
中文
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T-Helper Type-2 Contact Hypersensitivity of Balb/c Mice Aggravated by Dibutyl Phthalate via Long-Term Dermal Exposure
作者：
Li, Jinquan;Li, Li;Zuo, Haoxiao;Ke, Chenjuan;Yan, Biao;...
期刊：
PLOS ONE ,2014年9(2):e87887 ISSN：1932-6203
通讯作者：
Yang, Xu
作者机构：
[Li, Li; Li, Jinquan; Ke, Chenjuan; Zuo, Haoxiao; Wen, Huaxiao; Yang, Xu; Yan, Biao] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.;[Zhang, Yinping] Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.
通讯机构：
[Yang, Xu] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.
关键词：
Ears;Mouse models;Cytokines;Phthalates;Enzyme-linked immunoassays;Allergies;Inflammation;Eosinophils
摘要：
OBJECTIVE: During the last few decades, the prevalence of allergic skin diseases, asthma and rhinitis, has increased worldwide. Introduction of environmental chemicals with aggravation effects may play a part in this increase. The artificial chemical product dibutyl phthalate (DBP) is used in many products used in daily life. Dermal exposure to DBP is a common (but easily neglected) exposure pattern. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we examined the aggravation effect of long-term dermal exposure to DBP in a T-helper type 2 (Th2) model of contact hypersensitivity (CHS) in mice, and sought the potential molecular mechanisms. Experimental tests were conducted after 40-day dermal exposure to saline or three concentrations of DBP and subsequent three times of sensitization with 0.5% fluorescein isothiocyanate (FITC) or vehicle. The results of immunological and inflammatory biomarkers (total-immunoglobulin (Ig)E and Th cytokines) as well as histopathological examination and measurement of ear swelling supported the notion that high doses of DBP may promote and aggravate atopic dermatitis. Increased expression of thymic stromal lymphopoietin (TSLP) in this mouse model suggested that TSLP might be one of the molecular mechanisms of the aggravation effect induced by DBP. CONCLUSIONS/SIGNIFICANCE: Together, these results indicated that long-term dermal exposure to types of environmental toxins such as phthalates may endow an atopic predisposition in animals or humans. In addition, the high expression of TSLP in the mouse model demonstrated that TSLP might have an important role in the aggravation effect. This result could help to provide effective prevention strategies against atopic diseases such as atopic dermatitis (AD).
语种：
英文
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Oxidative damage of brain induced by nano-CdSeS in mice
作者：
Yang, Shangyue;Ye, Ranfeng;Cai, Wenjun;Xiang, Xiaoling;Yang, Xu*
期刊：
Advanced Materials Research ,2013年669:316-324 ISSN：1022-6680
通讯作者：
Yang, Xu
作者机构：
[Yang, Xu; Yang, Shangyue] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.;Huazhong Normal Univ, Lab Environm Biomed, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Xu] H;Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
11th China International Nanoscience and Technology Symposium (CINST)
会议时间：
OCT 21-25, 2012
会议地点：
Kunming, PEOPLES R CHINA
会议主办单位：
[Yang, Shangyue] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.^Huazhong Normal Univ, Lab Environm Biomed, Coll Life Sci, Wuhan 430079, Peoples R China.
会议论文集名称：
Advanced Materials Research
关键词：
nano-CdSeS;superoxide dismutase (SOD);malondialdehyde (MDA);comet assay;oxidative damage;mouse;brain
摘要：
In this experiment, the oxidative damage of nano-CdSeS in mice brains was performed. 20 male Kunming mice were divided into 4 groups and 3 experimental groups were exposed to different doses of nano-CdSeS (0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL) by intravenous administration while the control used saline solution instead. Three days later, the enzymatic activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the damage degree of DNA were determined to assess the oxidative damage in brain tissues. Our results showed that in the experimental groups, SOD activity was inhibited and MDA content was increased as the doses rising, at the same time, tail moment and tail DNA% increased significantly when comparing with the control. And these results exhibited a certain doses-dependency relations. From results above, it demonstrated that oxidative damage of brain induced by nano-CdSeS which enter into blood–brain barrier in mice.
语种：
英文
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某高校糖尿病及糖尿病前期患病趋势的统计分析
作者：
张振业;周鄂生;杨旭
期刊：
公共卫生与预防医学 ,2013年24(4):18-20 ISSN：1006-2483
作者机构：
华中师范大学校医院,武汉,430079;华中师范大学生命科学学院
关键词：
糖尿病;糖尿病前期;患病率变化趋势
摘要：
目的 对武汉市某高校社区教职员工糖尿病近3年(2010-2012年)的变化趋势进行了分析,以便针对性的进行卫生管理、预防和健康教育.方法 调查数据来自本医院健康体检所获得的血糖检测数据.结果 2012年糖尿病患病率7.14％(302/4229),较2010年的4.44％(182/4 103)显著增加；2012年糖尿病前期患病率19.58％(828/4229),较2010年的7.53％(309/4 103)显著增加；两个年度糖尿病和糖尿病前期均出现＞50岁人群是患病主体；男性多于女性；年轻人患病率增加的情况.结论 该高校教职员工糖尿病患病率逐年增多,且有年轻化趋势；应加强对重点人群进行定期检测、开展积极有效的健康教育.
语种：
中文
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甲醛对小鼠骨髓组织中GATA-2和C/EBPα表达的影响
作者：
张玉超;刘旭东;李慧;杨旭;丁书茂
期刊：
环境与健康杂志 ,2013年30(8):659-661 ISSN：1001-5914
作者机构：
[张玉超; 刘旭东; 李慧; 杨旭; 丁书茂] 华中师范大学生命科学学院环境生物医学实验室
关键词：
甲醛;骨髓;造血;转录因子
摘要：
目的探讨吸入式甲醛暴露对小鼠骨髓组织中转录因子GATA-2和CCAAT/增强子结合蛋白(C/EBPα)在mRNA以及蛋白表达水平的影响。方法将24只Balb/C小鼠随机分为3组,每组8只,采用仿真式口鼻暴露的方式,暴露于不同浓度的气态甲醛环境中(0、0.5和3.0mg/m3),每天8h,连续2周。染毒结束后,分别采用反转录PCR(RT-PCR)和酶联免疫吸附(ELISA)法测定小鼠骨髓组织中转录因子GATA-2和C/EBPa mRNA和蛋白的表达。结果与对照组相比,转录因子GATA-2和C/EBPα mRNA和蛋白的表达均随甲醛浓度的升高呈下降趋势,且甲醛浓度为3.0 mg/m3时,差异有统计学意义(P<0.05)。结论高浓度(3.0 mg/m3)甲醛暴露可以抑制小鼠骨髓组织中GATA-2和C/EBPα mRNA和蛋白的正常表达。
语种：
中文
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Isolation and characterization of two n-butyl benzyl phthalate degrading bacteria
作者：
Yang, Xue;Zhang, Can;He, Zhen;Hu, Xiaojing;Guo, Jia;...
期刊：
International Biodeterioration & Biodegradation ,2013年76:8-11 ISSN：0964-8305
通讯作者：
Xiong, Li
作者机构：
[Zhong, Qiu; Xiong, Li; He, Zhen; Liu, Deli; Yang, Xue; Hu, Xiaojing; Zhang, Can; Guo, Jia; Wang, Jinlong] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Xiong, Li] H;Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Biodegradation;Isolation and identification;N-Butyl benzyl phthalate (BBP)
摘要：
nButyl benzyl phthalate (BBP) is a phthalic acid ester (PAE) used extensively in the manufacturing of plastics that contributes to the environmental pollution. It easily released to the environment and resistant to degradation. Bioremediation has been proposed for removal of PAEs from contaminated sites. Studies showed that the biodegradation of PAEs was slow if the lateral chain was longer and more complex. Two BBP degrading bacterial strains, HS-B1, HS-B2, were isolated from contaminated sludge in the river of Jingzhou in Hubei province. The two bacteria were identified as Acinetobacter sp. and Arthrobacter sp. by morphology and 16S rDNA sequence analysis. BBP at 500 mg L<sup>-1</sup> in the culture medium was tested and after 24 h of culture, chloroform was used to extract the BBP in the culture medium. The initial and the final concentrations of BBP were assessed by UV-visible spectrophotometer. The results showed that the bacterium could grow well on BBP as the sole source of carbon and energy. The degradation of 500 mg L<sup>-1</sup> BBP by strains HS-B1 and HS-B2 was 28% and 59% after 24 h, respectively. The rate of degradation increased to 40%, 75% respectively with addition of 0.1% LB in 24 h, suggesting that additional sources of growth factors enhanced biodegradation. This study is helpful to the development of microbial resources for bioremediation. ©2012 Elsevier Ltd.
语种：
英文
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