Single-wall carbon nanotube-induced airway hyperresponsiveness in rats and a postulated mechanism of action
作者：
Li, Jinquan* ;Li, Li;Liu, Xudong;Li, Rui;Yang, Xu
期刊：
RSC Advances ,2013年3(47):25388-25395 ISSN：2046-2069
通讯作者：
Li, Jinquan
作者机构：
[Li, Li; Li, Jinquan; Li, Rui; Yang, Xu; Liu, Xudong] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Jinquan] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
摘要：
Inflammatory responses, oxidative stress, as well as fibrosis in lungs induced by a type of nano-sized carbon nanotubes (single-walled carbon nanotubes (SWCNTs)) have been investigated. However, studies examining the effects of SWCNTs on airway hyperresponsiveness (AHR) are not so numerous. We examined the effects of pulmonary exposure to SWCNTs on AHR and sought the underlying molecular mechanisms. Wistar rats were divided into four experimental groups and repeatedly administered vehicle or 0.02, 0.20 and 2.00 mg kg<sup>-1</sup> body weight SWCNTs through the intratracheal route. We found that 0.20 and 2.00 mg kg<sup>-1</sup> body weight SWCNTs in rats caused significant exacerbation of AHR. SWCNTs also amplified the lung protein levels of T helper 2 cytokines, immunoglobulin production in serum, profiles of immune cells in lungs, and levels of oxidative stress-related biomarkers in the airways, and exhibited airway remodelling. These results suggest that SWCNTs can exacerbate AHR in rats by enhancing airway inflammation and pulmonary oxidative stress. This journal is ©The Royal Society of Chemistry.
语种：
英文
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Effects of home environment and lifestyles on prevalence of atopic eczema among children in Wuhan area of China
作者：
Zhang Ming;Wu Yang;Yuan Ye;Zhou ESheng;Sundell, Jan;...
期刊：
科学通报(英文版) ,2013年58(34):4217-4222 ISSN：2095-9273
通讯作者：
Sun YueXia
作者机构：
[Zhang Ming; Wu Yang; Yang Xu; Yuan Ye] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhou ESheng] Cent China Normal Univ, Univ Hosp, Dept Publ Hlth, Wuhan 430079, Peoples R China.;[Sundell, Jan] Tsinghua Univ, Dept Bldg Sci, Beijing 100081, Peoples R China.;[Sun YueXia] Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China.
通讯机构：
[Sun YueXia] T;Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China.
关键词：
children;eczema;home environment;building characteristics;dampness;indoor air quality
摘要：
Atopic eczema is a common health problem in children. The prevalence has increased in the past decades. Besides a genetic predisposition, lifestyle factors, dietary habits and indoor environments are thought to be related to its high prevalence. A cross-sectional questionnaire study was carried out in 2011 in the Wuhan area to study associations between home environments and children's health. The questionnaires were replied to by parents of 2193 children 1-8 years old and the response rate was 91.4% (2193/2400). The results show: (1) The prevalence of "eczema symptom ever that lasted for 6 months" was 23.2%; "eczema symptom in the last 12 months" 7.4% and "awake at night due to itchy rash" 3.4%. (2) Risk factors: Closer to business area (AOR 1.4), moisture related problems (AOR 1.3-2.2), moldy and stuffy odor perception (AOR 1.5), mice observed (AOR 1.8), pet keeping (AOR 1.3), exposure to environmental tobacco smoke (AOR 1.3) and incense (AOR 1.8) were significant risk factors for atopic eczema symptoms ever. Living in an apartment, compared to single family house, was related to more eczema symptom in the last 12 months. Damp clothing/bed sheets and perception of stuffy odor were risk factors for both "eczema symptom in the last 12 months" and "awake due to itchy rash (severe eczema)". The odds ratio of mice observed for severe eczema was 3.1 (95% CI: 1.4-6.7). It suggests that urbanization, life style and dampness problems at home were significant risk factors for eczema symptoms among children in Wuhan, China. © 2013 The Author(s).
语种：
英文
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苯和甲醛联合染毒对小鼠脾脏的损伤
作者：
问华肖;魏晨曦;陈绍恢;毛琳;杨旭
期刊：
生态毒理学报 ,2013年8(4):571-576 ISSN：1673-5897
作者机构：
华中师范大学生命科学学院 遗传调控与整合生物学湖北省重点实验室,武汉,430079;[魏晨曦; 杨旭; 问华肖; 毛琳; 陈绍恢] 华中师范大学
关键词：
苯;甲醛;小鼠;脾脏
摘要：
为了探讨苯和甲醛联合染毒对小鼠脾脏的损伤以及二者是否具有一定的协同作用,选择BALB/c雄性小鼠30只,随机分为5组,每组6只,设苯组、甲醛组、苯和甲醛联合染毒组及玉米油对照组和空白对照组,对小鼠进行气态甲醛吸入染毒或/和苯玉米油溶液灌胃染毒.染毒结束后对小鼠脾脏进行组织病理学观察,并且测定脾脏的脏器系数以及脾脏组织的活性氧(ROS)和丙二醛(MDA)的含量.结果显示,苯和甲醛联合染毒组小鼠脾脏组织中ROS的含量与空白对照组相比有极显著的上升趋势(P ＜0.01),脾脏的脏器系数以及脾脏组织中MDA的含量与空白对照组相比有显著性差异(P＜0.05).此外,在脾脏免疫组织形态变化以及脾脏组织中MDA含量这2个生理生化指标上,苯和甲醛联合染毒对小鼠脾脏的损伤具有一定的协同作用.
语种：
中文
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邻苯二甲酸酯相关疾病导向性分子毒理学研究
作者：
杨旭
作者机构：
[杨旭] 华中师范大学生命科学学院环境生物医学实验室
会议名称：
环境与健康——从科研到政策国际论坛暨中国环境科学学会环境医学与健康分会2013年会
会议时间：
2013-8-12
会议地点：
太原
会议主办单位：
中国环境科学学会
会议论文集名称：
环境与健康——从科研到政策国际论坛暨中国环境科学学会环境医学与健康分会2013年会论文集
关键词：
毒理学;邻苯二甲酸酯;污染暴露;疾病导向性分子
摘要：
目的:采用分子毒理学研究技术,探讨环境邻苯二甲酸酯污染对人体健康的影响及其相关机理.方法:以环境邻苯二甲酸酯相关疾病为线索和导向进行分子毒理学研究,用接近实际环境中邻苯二甲酸酯的暴露水平进行染毒,重点研究处于"暴露"和"终点效应"之间的"分子事件"以及它们的"上下游关系",揭示邻苯二甲酸酯相关疾病可能的发病机理.结果:以其他课题组已经发表的人群流行病学研究报告为线索，所关注的疾病包括:过敏性哮喘、接触性皮炎、自闭症、儿童学习障碍、抑郁症、糖尿病、肥胖症,孕妇甲状腺功能减退及其相关性儿童智障。在实验动物疾病模型的基础上，已经发现了一系列的相关关系，及其可能的致病机理。结论:(1)邻苯二甲酸酯暴露与多种动物疾病模型的恶化相关;(2)分子毒理学研究是阐明致病机制的重要手段。
语种：
中文
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Oxidative damage induced by chlorpyrifos in the hepatic and renal tissue of Kunming mice and the antioxidant role of vitamin E
作者：
Ma, Ping;Wu, Yang;Zeng, Qiang;Gan, Yaping;Chen, Jiaoe;...
期刊：
Food and Chemical Toxicology ,2013年58:177-183 ISSN：0278-6915
通讯作者：
Yang, Xu
作者机构：
[Chen, Jiaoe; Gan, Yaping; Ma, Ping] Hubei Univ Sci & Technol, Coll Basic Med Sci, Xianning 437100, Peoples R China.;[Wu, Yang; Ye, Xin; Yang, Xu; Ma, Ping; Zeng, Qiang] Cent China Normal Univ, Coll Life Sci, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Yang, Xu] Huazhong Normal Univ, Coll Life Sci, Lab Environm Biomed, Bldg 5,152 Luo Yu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Xu] H;Huazhong Normal Univ, Coll Life Sci, Lab Environm Biomed, Bldg 5,152 Luo Yu Rd, Wuhan 430079, Peoples R China.
关键词：
8-Hydroxy-2-deoxyguanosine;Chlorpyrifos;DNA-protein crosslinks;Malondialdehyde;Oxidative stress;Vitamin E
摘要：
Chlorpyrifos is a broad-spectrum, chlorinated organophosphate pesticide employed for pest control in various agricultural and animal husbandries. Acute and chronic exposure to CPF can elicit several adverse effects, including oxidative stress. We investigated neurotoxicity of CPF-treated mice, and evaluated the antioxidant effect of vitamin E against oxidative stress and histological changes in the livers and kidneys of CPF-treated mice. Kunming mice were divided randomly into five exposure groups of six: (A) peanut oil; (B) 3. mg/kg CPF; (C) 6. mg/kg CPF; (D) 12. mg/kg CPF; (E) vitamin E (100. mg/kg), 3. h after administration of CPF (12. mg/kg) and used as a post-treatment group. Oral administration of high-dose groups (12. mg/kg) CPF led to a significant increase in levels of reactive oxygen species, DNA-protein crosslinks, 8-hydroxy-2-deoxyguanosine and malondialdehyde, decreases in acetylcholinesterase activity and glutathione level, as well as causing hepatic and renal histopathological change. Except for AChE activity levels, administration of vitamin E to CPF-treated mice restored these biochemical parameters to within normal levels, and resulted in overall improvement in damage to livers and kidneys. These data suggest that oxidative stress is involved in CPF-induced toxicity and that vitamin E can protect against the tissue damage induced by CPF. © 2013 Elsevier Ltd.
语种：
英文
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农药毒死蜱对小鼠肺细胞氧化损伤的研究
作者：
马萍;焦铭;刘旭东;刘锋明;杨旭;...
期刊：
华中师范大学学报(自然科学版) ,2013年47(2):246-249 ISSN：1000-1190
作者机构：
湖北科技学院基础医学院,湖北咸宁437100;华中师范大学生命科学学院,武汉430079;湖北科技学院基础医学院,湖北咸宁,437100;华中师范大学生命科学学院,武汉,430079;[刘旭东; 杨旭; 刘锋明; 丁书茂] 华中师范大学
关键词：
毒死蜱;小鼠;肺脏;氧化损伤
摘要：
为了研究毒死蜱对生物体的氧化损伤,以昆明小鼠为受试体,毒死蜱按3、6和12 mg/kg 3个剂量水平,灌胃染毒小鼠,每天1次,连续7d,测定小鼠肺的脏器系数比,以肺组织匀浆液测定活性氧(reactive oxygen species,ROS)、还原型谷胱甘肽(glutathione,GSH)和丙二醛(Malondialdehyde,MDA)的含量.实验结果表明:随着毒死蜱染毒剂量的升高,ROS和MDA含量逐渐上升,肺的脏器系数比和GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量≥6 mg/kg时,GSH和MDA含量有显著差异(P＜0.05)；染毒剂量≥12mg/kg时,ROS含量有显著差异(P＜0.05),肺的脏器系数比、GSH和MDA含量有极显著差异(P＜0.01).说明较高剂量的毒死蜱可造成小鼠肺组织的氧化损伤.
语种：
中文
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甲醛对原癌基因c-myc、MDM2及抑癌基因p53的影响
作者：
李慧;张玉超;柯玉洁;秦晓丹;刘旭东;...
期刊：
中国环境科学 ,2013年33(8):1483-1486 ISSN：1000-6923
通讯作者：
Ding, S.-M.(dingsm@mail.ccnu.edu.cn)
作者机构：
[刘旭东; 熊棣; 李慧; 杨旭; 柯玉洁; 问华肖; 张玉超; 丁书茂; 秦晓丹; 李睿] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, China
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, China
关键词：
甲醛;SPF级Balb/c纯系雄性小鼠;骨髓组织
摘要：
为研究吸入性甲醛的毒性能否进入动物骨髓组织,引起骨髓组织基因表达发生改变,选择小鼠某些原癌基因和抑癌基因为研究对象,以SPF级balb/c雄性小鼠为材料,采用动态吸入方式染毒2周(5+2模式),染毒浓度分别为0,0.5,3.0mg/m~3,用半定量RT-PCR方法检测不同浓度甲醛对小鼠染毒后骨髓组织细胞中c-myc、MDM2和p53基因表达的变化.结果表明,在不同浓度的甲醛暴露条件下,与空白对照组相比,小鼠骨髓组织中的c-myc基因,MDM2基因和p53基因表达均发生改变,在3.0mg/m~3浓度组与空白对照组存在显著差异(P<0.05),c-myc基因,MDM2基因呈现表达上调,p53基因则呈现表达下调.吸入性甲醛的毒性能进入动物骨髓组织,并能引起骨髓组织基因表达发生改变.
语种：
中文
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杏鲍菇培养料中细菌系统发育多样性
作者：
项晓玲;李岩;田熙科;杨超;吴曲;...
期刊：
西南农业学报 ,2013年26(4):1589-1592 ISSN：1001-4829
作者机构：
[项晓玲; 李岩; 吴曲; 常青; 杨旭] 华中师范大学生命科学学院环境科学实验室;[田熙科; 杨超] （武汉）中国地质大学材料科学与化学工程学院;[袭著革] 军事医学科学院卫生学环境医学研究所
关键词：
杏鲍菇培养料;常压灭菌;细菌;系统发育分析
摘要：
为了探讨纳米硫硒化镉(CdSeS)对小鼠肾脏和脑组织的急性氧化损伤作用,将20只雄性昆明小鼠随机分成4组,采用尾部静脉注射进行一次性染毒,3个染毒组分别注人0.1、0.2、0.4mg·mL~(-1)的纳米CdSeS粉末(20～30nm)悬液1mL,对照组注入等体积生理盐水.染毒3d后对肾脏和脑组织匀浆中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量分别进行测定,从而检测纳米CdSeS对肾脏和脑组织的急性氧化损伤作用.结果显示,随着纳米CdSeS染毒浓度的升高,小鼠肾脏和脑组织中SOD活力呈逐渐降低趋势,而MDA含量呈逐渐升高趋势,均显示出一定的剂量-效应关系;较高浓度组(肾:ρ_(CdSeS)≥0.2mg·mL~(-1);脑:ρ_(CdSeS)≥0.4mg·mL-1)SOD活力、MDA含量与对照具有显著性差异(p<0.05,p<0.01),而较低浓度组则没有显著性差异(p0.05).以上结果提示纳米CdSeS能够对小鼠肾脏和脑组织造成氧化损伤,并且能穿过血脑屏障作用于脑部.
语种：
中文
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Effects of silica dioxide nanoparticles on the embryonic development of zebrafish
作者：
Ye, Ranfeng;Yu, Xiaowei;Yang, Shangyue;Yuan, Junlin;Yang, Xu*
期刊：
Integrated Ferroelectrics ,2013年147(1):166-174 ISSN：1058-4587
通讯作者：
Yang, Xu
作者机构：
[Yang, Xu] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;Huazhong Normal Univ, Coll Life Sci, Lab Environm Biomed, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Xu] H;Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Malondialdehyde;Silica dioxide nanoparticles;Toxicity;Zebrafish embryos
摘要：
To assess the aquatic ecosystem safety of silicon dioxide (SiO2) nanoparticles (NPs), we studied the toxicity of the SiO2 NPs and SiO2 bulk particles (BPs) using zebrafish. Our results show that the SiO2 NPs can significantly affect the development of zebrafish embryos, while there is no obvious effect on survival and development of embryos was found in SiO2 BPs exposure group. The content of malondialdehyde (MDA) in zebrafish larvae exposed to the SiO2 NPs for 108 hours, increased significantly in dose-dependent manner. These results suggest that the SiO2 NPs have toxic effects on the development of zebrafish embryos and the effects could be resulted by oxidative damage. Copyright © 2013 Taylor and Francis Group, LLC.
语种：
英文
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邻苯二甲酸二丁酯(DBP)与卵清白蛋白(OVA)联合染毒对小鼠肺脏和脾脏组织氧化应激
作者：
陈绍恢;尤会会;毛琳;问华肖;叶染枫;...
期刊：
生态毒理学报 ,2013年8(4):537-542 ISSN：1673-5897
作者机构：
[陈绍恢; 尤会会; 毛琳; 问华肖; 叶染枫; 杨旭] 华中师范大学生命科学学院
关键词：
邻苯二甲酸二丁酯;卵清白蛋白;小鼠;氧化应激
摘要：
为研究邻苯二甲酸二丁酯(dibutyl phthalate, DBP)单独染毒及与卵清白蛋白(ovalbumin, OVA)联合染毒对小鼠肺脏和脾脏组织氧化应激的作用,将BALB/c小鼠随机分为8组:(1)未处理对照组(生理盐水组);(2) 0.5 mg·kg~(-1)DBP染毒组;(3) 5.0 mg·kg~(-1) DBP染毒组;(4) 50 mg·kg~(-1) DBP染毒组;(5) 1.67 mg·kg~(-1) OVA单独染毒组;(6) 0.5 mg·kg~(-1) DBP与1.67 mg·kg~(-1) OVA联合染毒组;(7) 5.0 mg·kg~(-1) DBP与1.67 mg·kg~(-1) OVA联合染毒组;(8) 50 mg·kg~(-1) DBP与1.67 mg·kg~(-1) OVA联合染毒组。未处理对照组和DBP染毒组每天按体质量给予生理盐水和DBP灌胃。2周后,测定肺脏组织活性氧物种(ROS)、谷胱甘肽(GSH)和丙二醛(MDA)含量以及脾脏组织ROS、GSH含量。结果显示,联合染毒组相较于其他组的肺脏组织各指标均有不同的显著性差异(P<0.05),联合染毒组的脾脏组织中ROS含量较其他组有显著差异(P<0.05),而GSH含量无统计学意义(P>0.05)。结果说明,DBP与OVA联合染毒能够增强肺脏组织的氧化应激作用,对于脾脏组织的氧化应激作用不明显;DBP在联合染毒中显示一定免疫佐剂效应。
语种：
中文
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Bone Marrow Injury Induced via Oxidative Stress in Mice by Inhalation Exposure to Formaldehyde
作者：
Zhang, Yuchao;Liu, Xudong;McHale, Cliona;Li, Rui;Zhang, Luoping;...
期刊：
PLOS ONE ,2013年8(9):e74974 ISSN：1932-6203
通讯作者：
Ding, Shumao
作者机构：
[Wu, Yang; Ye, Xin; Li, Rui; Yang, Xu; Zhang, Yuchao; Liu, Xudong; Ding, Shumao] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.;[McHale, Cliona; Zhang, Luoping] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Genes & Environm Lab, Berkeley, CA 94720 USA.
通讯机构：
[Ding, Shumao] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.
关键词：
Oxidative stress;Apoptosis;Bone marrow;Transcription factors;Toxicity;Inhalation;Inflammation;Leukemia
摘要：
OBJECTIVE: Formaldehyde, a ubiquitous environmental pollutant has been classified as a human leukemogen. However, toxicity of formaldehyde in bone marrow, the target site of leukemia induction, is still poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: To investigate bone marrow toxicity (bone marrow pathology, hematotoxicity) and underlying mechanisms (oxidative stress, inflammation, apoptosis) in formaldehyde-exposed mice. Male Balb/c mice were exposed to formaldehyde (0, 0.5, and 3.0 mg/m(3)) by nose-only inhalation for 8 hours/day, over a two week period designed to simulate a factory work schedule, with an exposure-free "weekend" on days 6 and 7, and were sacrificed on the morning of day 13. Counts of white blood cells, red blood cells and lymphocytes were significantly (p<0.05) decreased at 0.5 mg/m(3) (43%, 7%, and 39%, respectively) and 3.0 mg/m(3) (52%, 27%, and 43%, respectively) formaldehyde exposure, while platelet counts were significantly increased by 109% (0.5 mg/m(3)) and 67% (3.0 mg/m(3)). Biomarkers of oxidative stress (reactive oxygen species, glutathione depletion, cytochrome P450 1A1 and glutathione s-transferase theta 1 expression), inflammation (nuclear factor kappa-B, tomour necrosis factor alpha, interleukin-1 beta), and apoptosis (activity of cysteine-aspartic acid protease 3) in bone marrow tissues were induced at one or both formaldehyde doses mentioned above. CONCLUSIONS/SIGNIFICANCE: Exposure of mice to formaldehyde by inhalation induced bone marrow toxicity, and that oxidative stress, inflammation and the consequential apoptosis jointly constitute potential mechanisms of such induced toxicity.
语种：
英文
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氯氰菊酯对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用
作者：
马萍;陈姣娥;甘亚平;曾强;毛琳;...
期刊：
环境与职业医学 ,2013年30(6):465-470 ISSN：2095-9982
作者机构：
湖北科技学院基础医学院,湖北437100;华中师范大学生命科学学院环境生物医学实验室,湖北430079;湖北科技学院基础医学院,湖北,437100;华中师范大学生命科学学院环境生物医学实验室,湖北,430079
关键词：
氯氰菊酯;活性氧;还原型谷胱甘肽;丙二醛;DNA-蛋白质交联;氧化损伤
摘要：
[目的]研究氯氰菊酯(CP)对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用. [方法]以36只昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯(40mg/kg)加维生素E组(100 mg/kg),染毒组按10、20和40 mg/kg 3个剂量水平,维生素E的剂量为100mg/kg,小鼠灌胃染毒7d.取睾丸称重测定脏器系数；以睾丸组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量；以睾丸细胞测定DNA-蛋白质交联(DPC)系数；并制作睾丸组织切片,观察其病理损伤. [结果]随着氯氰菊酯染毒剂量的升高,睾丸的脏器系数、ROS、MDA含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.与对照组比较,染毒剂量为20mg/kg时,睾丸脏器系数(0.568±0.027)、ROS含量(586.2±43.94)、GSH含量(18.15±2.351)、DPC系数(0.186±0.017)和MDA含量(2.051±0.137)差异有统计学意义(P＜0.05,P＜0.01),睾丸部分生精小管生精细胞减少,生精细胞脱落管腔；染毒剂量为40 mg/kg时,GSH含量(17.13±1.203)、脏器系数(0.643±0.028)、ROS含量(898.8±100.23)、MDA含量(2.299±0.157)和DPC系数(0.229±0.020)差异有统计学意义(P＜0.05,P＜0.01),睾丸生精细胞明显减少,部分生精细胞脱落坏死,阻塞管腔.高剂量染毒加维生素E组与高剂量染毒组相比较,睾丸的脏器系数、ROS、MDA含量和DPC系数均有下降,GSH含量上升.ROS含量(550.4±102.3)、GSH含量(24.65±2.892)和MDA含量(1.291±0.289)差异有统计学意义(P＜0.05)；睾丸的脏器系数(0.507±0.026)和DPC系数(0.139±0.017)差异有统计学意义(P＜0.01). [结论]较高剂量(20、40 mg/kg)的氯氰菊酯能造成小鼠睾丸的氧化损伤和病理损伤,且可以被维生素E的抗氧化作用所拮抗.
语种：
中文
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纳米二氧化锆超细颗粒物对KB细胞活性的影响
作者：
晏彪;胡浩;陈绍恢;刘旭东;闵安娜;...
期刊：
公共卫生与预防医学 ,2013年24(3):5-8 ISSN：1006-2483
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉430079;华中师范大学物理与纳米技术学院
关键词：
纳米氧化锆;KB细胞;生物相容性;MTT法
摘要：
目的评估纳米二氧化锆（nano-ZrO2）对人类口腔细胞活性的影响。方法采用不同浓度的纳米ZrO2颗粒（0、10、30、60、100、150、250μg.ml-1）对Human epidermoid carcinoma（KB）细胞分别进行24、48、72 h的染毒,应用MTT法检测细胞活性。结果①24、48 h低浓度纳米ZrO2染毒时KB细胞活性较高;②24、48 h高浓度nano-ZrO2染毒时没有对KB细胞的活性产生明显影响;③染毒72 h之后KB细胞活性没有发生显著变化,nano-ZrO2对KB细胞也不具有细胞毒性。结论在适当浓度下,nano-ZrO2对KB细胞活性无明显毒作用。
语种：
中文
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武汉地区室内环境质量与儿童哮喘和过敏性鼻炎患病率的关系
作者：
张铭;周鄂生;叶新;孙越霞;杨旭
期刊：
科学通报 ,2013年58(25):2548-2553 ISSN：0023-074X
作者机构：
[张铭; 叶新; 杨旭] 华中师范大学生命科学学院;[周鄂生] 华中师范大学校医院公共卫生科;[孙越霞] 天津大学环境科学与工程学院;[Sundell Jan] 清华大学建筑技术科学系
关键词：
儿童;哮喘;鼻炎;家居环境;室内空气质量;武汉
摘要：
为了解与中国儿童哮喘和过敏患病率相关的室内危险因素, 2011年采用横断面问卷调查的方法在武汉地区调查了2400名年龄在1~8岁儿童的父母, 有效应答率为91.4%. 研究结果显示武汉地区儿童“近12个月内有过哮鸣”的患病率为18.5%, “近12个月内有过夜里干咳”的患病率为15.4%, “医生诊断为哮喘”的患病率为6.0%, “近12个月有过敏性鼻炎”的患病率为48.7%, “医生诊断为过敏性鼻炎”的患病率为17.5%. 与哮喘和过敏及相关症状增加有关的危险因素为生活在城市地区、住宅潮湿、使用天然气做饭、重新装修(怀孕期间使用涂料和添置家具)、饲养宠物、哺乳期少于3个月. 卫生间安装排气扇可使危险度降低. 研究结果表明, 在武汉地区, 儿童哮喘和过敏性鼻炎的患病率与室内环境因素显著相关, 武汉地区城市化和住宅潮湿问题是儿童哮喘和过敏性鼻炎的重要的危险因素.
语种：
中文
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Inhaled formaldehyde induces DNA-protein crosslinks and oxidative stress in bone marrow and other distant organs of exposed mice
作者：
Ye, Xin;Ji, Zhiying;Wei, Chenxi;McHale, Cliona M.;Ding, Shumao;...
期刊：
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS ,2013年54(9):705-718 ISSN：0893-6692
通讯作者：
Zhang, Luoping
作者机构：
[Ye, Xin; Wei, Chenxi; Yang, Xu; Ding, Shumao] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Lab Environm Biomed, Wuhan 430079, Peoples R China.;[Thomas, Reuben; Zhang, Luoping; McHale, Cliona M.; Ji, Zhiying] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Genes & Environm Lab, Berkeley, CA 94720 USA.;[Zhang, Luoping] Univ Calif Berkeley, Sch Publ Hlth, Li Ka Shing Ctr 388, Div Environm Hlth Sci,Genes & Environm Lab, Berkeley, CA 94720 USA.
通讯机构：
[Zhang, Luoping] U;Univ Calif Berkeley, Sch Publ Hlth, Li Ka Shing Ctr 388, Div Environm Hlth Sci,Genes & Environm Lab, Berkeley, CA 94720 USA.
关键词：
formaldehyde;DPC;oxidative stress;leukemia;bone marrow toxicity
摘要：
Formaldehyde (FA), a major industrial chemical and ubiquitous environmental pollutant, has been classified as a leukemogen. The causal relationship remains unclear, however, due to limited evidence that FA induces toxicity in bone marrow, the site of leukemia induction, and in other distal organs. Although induction of DNA-protein crosslinks (DPC), a hallmark of FA toxicity, was not previously detected in the bone marrow of FA-exposed rats and monkeys in studies published in the 1980s, our recent studies showed increased DPC in the bone marrow, liver, kidney, and testes of exposed Kunming mice. To confirm these preliminary results, in the current study we exposed BALB/c mice to 0, 0.5, 1.0, and 3.0 mg m-3 FA (8 hr per day, for 7 consecutive days) by nose-only inhalation and measured DPC levels in bone marrow and other organs of exposed mice. As oxidative stress is a potential mechanism of FA toxicity, we also measured glutathione (GSH), reactive oxygen species (ROS), and malondialdehyde (MDA), in the bone marrow, peripheral blood mononuclear cells, lung, liver, spleen, and testes of exposed mice. Significant dose-dependent increases in DPC, decreases in GSH, and increases in ROS and MDA were observed in all organs examined (except for DPC in lung). Bone marrow was among the organs with the strongest effects for DPC, GSH, and ROS. In conclusion, exposure of mice to FA by inhalation induced genotoxicity and oxidative stress in bone marrow and other organs. These findings strengthen the biological plausibility of FA-induced leukemogenesis and systemic toxicity. © 2013 Wiley Periodicals, Inc.
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英文
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农药毒死蜱对小鼠脑细胞氧化损伤的研究
作者：
马萍;焦铭;尤会会;赵静云;杜娟;...
期刊：
环境科学学报 ,2013年33(3):867-871 ISSN：0253-2468
通讯作者：
Yang, X.
作者机构：
[马萍; 焦铭] 湖北科技学院基础医学院;[尤会会; 赵静云; 杜娟; 罗清; 杨旭] 华中师范大学生命科学学院环境科学实验室
通讯机构：
Lab of Environment Science, College of Life Science, Huazhong Normal University, China
关键词：
毒死蜱;小鼠;氧化损伤;DNA损伤
摘要：
毒死蜱是一种高效、低毒、广谱的有机磷杀虫杀螨剂.为研究其对生物体的氧化损伤,以昆明小鼠为受试体,毒死蜱按3、6和12 mg?kg~(-1)3个剂量水平灌胃染毒小鼠7 d,以脑组织匀浆测定活性氧(Reactive oxygen species,ROS)、还原型谷胱甘肽(Glutathione,GSH)和丙二醛(Malondialdehyde,MDA)的含量,以脑细胞测定DNA-蛋白质交联(DNA-protein Crosslink,DPC)系数.实验结果表明,随着毒死蜱染毒剂量的升高,ROS和MDA含量及DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量为6 mg?kg~(-1)时,与对照组相比,DPC有显著差异(p<0.05),MDA有极显著差异(p<0.01);染毒剂量为12 mg?kg~(-1)时,与对照组相比,ROS和DPC有显著差异(p<0.05),GSH和MDA有极显著差异(p<0.01).说明较高剂量的毒死蜱可造成小鼠脑组织的氧化损伤和DNA-蛋白质交联作用增强.
语种：
中文
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Role of Transient Receptor Potential Ion Channels and Evoked Levels of Neuropeptides in a Formaldehyde-Induced Model of Asthma in Balb/c Mice
作者：
Wu, Yang;You, Huihui;Ma, Ping;Li, Li;Yuan, Ye;...
期刊：
PLOS ONE ,2013年8(5):e62827 ISSN：1932-6203
通讯作者：
Lai, Kefang
作者机构：
[Wu, Yang; Li, Li; Ye, Xin; Yuan, Ye; Yao, Hanchao; Li, Jinquan; You, Huihui; Yang, Xu; Liu, Xudong; Ma, Ping] Cent China Normal Univ, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Peoples R China.;[Wu, Yang; Lai, Kefang; Chen, Ruchong] Guangzhou Med Univ, Guangzhou Inst Resp Dis, State Key Lab Resp Dis, Guangzhou, Guangdong, Peoples R China.;[Ma, Ping] Hubei Univ Sci & Technol, Coll Basic Med Sci, Xianning, Peoples R China.
通讯机构：
[Lai, Kefang] G;Guangzhou Med Univ, Guangzhou Inst Resp Dis, State Key Lab Resp Dis, Guangzhou, Guangdong, Peoples R China.
关键词：
Asthma;Inflammation;Neuropeptides;Eosinophils;Cytokines;Enzyme-linked immunoassays;Transient receptor potential channels;Cell staining
摘要：
Objective: Asthma is a complex pulmonary inflammatory disease characterized by the hyper-responsiveness, remodeling and inflammation of airways. Formaldehyde is a common indoor air pollutant that can cause asthma in people experiencing long-term exposure. The irritant effect and adjuvant effect are the two possible pathways of formaldehyde promoted asthma. Methodology/Principal Findings: To explore the neural mechanisms and adjuvant effect of formaldehyde, 48 Balb/c mice in six experimental groups were exposed to (a) vehicle control; (b) ovalbumin; (c) formaldehyde (3.0 mg/m(3)); (d) ovalbumin+formaldehyde (3.0 mg/m(3)); (e) ovalbumin+formaldehyde (3.0 mg/m(3))+HC-030031 (transient receptor potential ankyrin 1 antagonist); (f) ovalbumin+formaldehyde (3.0 mg/m(3))+capsazepine (transient receptor potential vanilloid 1 antagonist). Experiments were conducted after 4 weeks of combined exposure and 1-week challenge with aerosolized ovalbumin. Airway hyper-responsiveness, pulmonary tissue damage, eosinophil infiltration, and increased levels of interleukin-4, interleukin-6, interleukin-1 beta, immunoglobulin E, substance P and calcitonin gene-related peptide in lung tissues were found in the ovalbumin+formaldehyde (3.0 mg/m(3)) group compared with the values seen in ovalbumin -only immunized mice. Except for interleukin-1 beta levels, other changes in the levels of biomarker could be inhibited by HC-030031 and capsazepine. Conclusions/Significance: Formaldehyde might be a key risk factor for the rise in asthma cases. Transient receptor potential ion channels and neuropeptides have important roles in formaldehyde promoted-asthma.
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英文
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Comparative study of the cytotoxicity of the nanosized and microsized tellurium powders on HeLa cells
作者：
Huanan WEN;Jiaxin ZHONG;Bei SHEN;Tao GAN;Chao FU;...
期刊：
生物学前沿 ,2013年8(4):444-450 ISSN：1674-7984
通讯作者：
Rui Li<&wdkj&>Xu Yang
作者机构：
[Wen H.; Gan T.; Shen B.; Zhong J.; Yang X.; Fu C.; Li R.] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, 430079, China;[Zhu Z.] Institute of Nanotechnology, College of Physical Science and Technology, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Rui Li; Xu Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China
关键词：
nanosized and microsized tellurium powder;HeLa cells;oxidative damage;reactive oxygen species (ROS);glutathione (GSH);DNA and protein crosslink (DPC);8-hydroxy-2’-deoxyguanosine (8-OHdG)
摘要：
To compare the cytotoxicity on HeLa cells induced by nanosized and microsized tellurium powders, HeLa cells were exposed to different concentrations of tellurium powders (0, 50, 100, 150 and 200 μg/mL) for 12 h. In this study, detection of a series of biomarkers, including reactive oxygen species (ROS), glutathione (GSH), 8-hydroxy-2′-deoxyguanosine (8-OHdG), in addition to DNA and protein crosslink (DPC) and MTTassay, were conducted to evaluate the cytotoxicity. It is indicated that compared with the control group, there was no significant difference in the induced cytotoxicity at concentrations lower than 50 μg/mL for both nanosized and microsized tellurium powders. While there appears a significant difference in the induced cytotoxicity for nanosized tellurium powders when the concentration is higher than 100 μg/mL as well as for microsized tellurium powders when the concentration is higher than 200 μg/mL. Moreover, it is found that the cytotoxicity induced on HeLa cells exhibits a certain dose-effect relationship with the concentration of tellurium powders. A conclusion has been reached that the toxicity on HeLa cells can be induced by both nanosized and microsized tellurium powders, and the toxicity of the nanosized tellurium powders is significantly greater than the microsized one. © 2013 Higher Education Press and Springer-Verlag Berlin Heidelberg.
语种：
英文
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三氧化钨纳米方块致小鼠肝脏和肾脏的急性氧化损伤
作者：
毛琳;尤会会;陈绍恢;问华肖;熊棣;...
期刊：
环境与健康杂志 ,2013年30(1):9-12 ISSN：1001-5914
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,湖北武汉430079;华中师范大学物理科学与技术学院,纳米科技研究院;[毛琳; 尤会会; 陈绍恢; 问华肖; 熊棣; 苏俊; 祝志宏; 杨旭; 李睿] 华中师范大学
关键词：
WO3纳米方块;急性氧化损伤;肝脏;肾脏;小鼠
摘要：
目的 为探讨WO3纳米方块对小鼠肝脏和肾脏组织的急性氧化损伤作用.方法 将42只Balb/c小鼠随机分为7组,每组6只,分别为5、10、20、40、80、160 mg/kg的WO3纳米方块染毒组和生理盐水对照组.灌胃染毒7d后测定肝脏和肾脏组织中活性氧(ROS)、还原性谷胱甘肽(GSH)以及丙二醛(MDA)的含量.结果 随着WO3纳米方块染毒剂量的升高,肝脏和肾脏组织的ROS和MDA的含量均呈逐渐上升的趋势,而GSH含量呈逐渐降低的趋势,各指标均呈一定的剂量-效应关系.剂量≥20 mg/kg时肝脏组织ROS含量和剂量≥10 mg/kg时肾脏组织ROS含量分别与对照组比较,差异有统计学意义(P＜0.05)；剂量≥20 mg/kg时,肝脏和肾脏组织MDA含量与对照组比较,差异有统计学意义(P＜0.05)；剂量≥40 mg/kg时肝脏组织GSH含量和剂量≥20 mg/kg时肾脏组织GSH含量分别与对照组比较,差异有统计学意义(P＜0.05).结论 一定剂量的WO3纳米方块能够对小鼠肝脏和肾脏造成急性氧化损伤.
语种：
中文
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Indoor environmental quality and the prevalence of childhood asthma and rhinitis in Wuhan area of China
作者：
Zhang Ming;Zhou ESheng;Ye Xin;Sun YueXia* ;Sundell, Jan;...
期刊：
科学通报(英文版) ,2013年58(34):4223-4229 ISSN：2095-9273
通讯作者：
Sun YueXia
作者机构：
[Zhang Ming; Yang Xu; Ye Xin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhou ESheng] Cent China Normal Univ, Univ Hosp, Dept Publ Hlth, Wuhan 430079, Peoples R China.;[Sun YueXia] Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China.;[Sundell, Jan] Tsinghua Univ, Dept Bldg Sci, Beijing 100081, Peoples R China.
通讯机构：
[Sun YueXia] T;Tianjin Univ, Sch Environm Sci & Engn, Tianjin 300072, Peoples R China.
关键词：
children;asthma;rhinitis;home environment;indoor air quality;Wuhan area
摘要：
Indoor environmental quality is suspected to be at least part of the cause of the increasing prevalence of childhood asthma and allergy. This study is part of the China, Children, Homes, Health (CCHH) project, which was designed to identify the risk factors indoors that are associated with prevalence of asthma and allergy among children in China. A cross-sectional questionnaire study was carried out in Wuhan in 2011. The questionnaire was a modified (to Chinese building characteristics) version of a questionnaire used first in Sweden. The parents of 2193 children, aged 1-8 years, replied, a response rate of 91.4% (2193/2400). Prevalences of asthma and allergic symptoms were calculated, and a Chi-square test and multiple logistic regression were used to identify risk factors. Prevalences of health outcomes are "wheezing last 12 months" 18.5%, "cough at night last 12 months" 15.4%, "doctor-diagnosed asthma" 6.0%, "rhinitis last 12 months" 48.7%, "doctor-diagnosed allergic rhinitis" 17.5%. Factors associated with increased risk for asthma, allergy and related symptoms include living in an urban area, dampness (significant), use of gas for cooking, new dwelling decoration (paint and furniture obtained during pregnancy), keeping pets and breast feeding less than 3 months. Factors associated with reduced risk as installing an exhaust fan in the bathroom. Home environmental factors are significantly associated with the prevalence of childhood asthma and rhinitis in Wuhan. Urbanization and dampness problems at home are significant risk factors for doctor diagnosed asthma and allergic rhinitis among children in Wuhan. © 2013 The Author(s).
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英文
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