作者机构：
College of Life Science, Central China Normal University, Wuhan 430079
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
Formaldehyde;Toxicological study;Physiological study
摘要：
This paper is a review on recent developments of the toxicological and physiological studies on formaldehyde.Hotspots involved in this topic include: (1) Formaldehyde-induced gene expression; (2) Formaldehyde-induced asthma; (3) Airway neurogenic inflammation (4) formaldehyde working as signaling molecule in body;, (5)Epigenesis: methylation of DNA and proteins; (6) DNA-protein crosslink; (7) Lysine-DPC repair enzyme; (8)Formaldehyde-induced Apoptosis.
作者机构：
[Yang, X.; Lu, Z.; Qiao, Y.; Yan, Y.] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Lu, Z.; Li, C. M.; Qiao, Y.; Yan, Y.] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore.
通讯机构：
[Yang, X.] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Formaldehyde;Learning;Memory;Morris water maze;NMDA receptor;Oxidative stress
摘要：
UNLABELLED: In this study, we investigated the effect of inhaled formaldehyde on learning and memory capacity. After exposure to 0 (control), 1 and 3 mg/m(3) of gaseous formaldehyde respectively, the behavior of mice in a Morris water maze, the expression of NR1, NR2B mRNA and oxidative damage levels in mice brain were analyzed. The water maze performance, the activities of dismutase superoxide (SOD) and levels of glutathione (GSH) decreased significantly in 3 mg/m(3) group (P < 0.01, compared with control group); while malondialdehyde (MDA) contents and expression of NR1 and NR2B genes increased significantly after exposure to 3 mg/m(3) of gaseous formaldehyde (P < 0.05, <0.01, <0.01, compared with control group). These findings indicate that inhaled formaldehyde negatively affects learning and memory at 3 mg/m(3) of gaseous formaldehyde but not at lower levels. Oxidative stress-induced neuron damages in the brain may be the possible mechanism for these effects. PRACTICAL IMPLICATIONS: This study indicates that inhaled formaldehyde starts to negatively affect learning and memory at a middle concentration of formaldehyde without interference of other indoor air pollutants. Oxidative damage, and the alteration of NMDA receptor expression, which were induced by formaldehyde inhalation, may be the possible mechanism for gaseous formaldehyde-induced neurotoxicity.
摘要：
In order to explore the molecular regulation of GSNO reductase (GSNOR), effects of environmental formaldehyde exposure and antioxidant alpha-lipoic acid (LA) injection on GSNOR activity and expression in mice lungs were investigated in the present study. Results showed that GSNOR activity was elevated upon 3.0 mg/m(3) FA exposure (compared with 0 mg/m(3) FA inhaled group, p<0.01) and the elevation was completely inhibited by LA injection (compared with 3.0 mg/m(3) FA inhaled group, p<0.01). Meanwhile, GSH levels were significantly decreased in 3.0 mg/m(3) FA inhaled group (compared with 0 mg/m(3) FA inhaled group, p<0.01), and were regenerated by LA injection (compared with 3.0 mg/m(3) FA inhaled group, p<0.01). The expression of GSNOR by using RT-PCR method correlated with the results of GSNOR activity assay. The results indicated that gaseous FA might activate GSNOR, and the activation may be related with the increase of oxidative stress. Since GSNOR has a central role in mediating SNO signaling and asthma development, these findings also suggested that indoor air pollutants such as FA might be key risk factors for the rise in asthma cases.
