期刊：
Journal of Bacteriology,2018年200(21):JB.00436-18 ISSN：0021-9193
通讯作者：
Qiu, Bao Sheri
作者机构：
[Zang, Sha-Sha; Li, Zheng-Ke; Qiu, Bao Sheri; Dai, Guo-Zheng; Liu, Ke; Song, Wei-Yu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Zang, Sha-Sha; Li, Zheng-Ke; Qiu, Bao Sheri; Dai, Guo-Zheng; Liu, Ke; Song, Wei-Yu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW, Australia.
通讯机构：
[Qiu, Bao Sheri] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
期刊：
PROTEIN JOURNAL,2018年37(6):531-538 ISSN：1572-3887
通讯作者：
Liu, Yanli
作者机构：
[Liu, Jinlin; Liang, Xiao; Yang, Xiajie; Gong, Siying; Li, Fangzhou; Qi, Chao; Lei, Ming; Liu, Ke; Li, Bing; Liu, Yanli; Zhou, Mengqi] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Cao, Yu] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Liu, Yanli] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
AL protein;PHD domain;Histone binding
摘要：
Alfin1-like (AL) is a family of proteins homologous to the alfalfa Alfin1 in plant and bears an Alfin domain and a PHD domain at their N- and C-terminus, respectively. There are 7 AL proteins in Arabidopsis, and the PHD domains of most AL proteins are reported to bind to histone H3K4me3. Here we reported gene cloning, protein expression and purification of the PHD domains of all the AL family proteins in Arabidopsis. We then systematically characterized their histone binding abilities by quantitative isothermal titration calorimetry and fluorescence polarization binding assays. Our binding results indicate that all the PHD domains of the AL proteins bind to the histone H3K4me3 peptide with varying methylation state preference and binding affinities. Our study presented here provides the foundation for further studies of the peptide state-specific recognition by PHD domains of AL proteins.
摘要：
Tumor remains a challenging task for oncology community. Drug resistance due to chemotherapy remain principal impediments toward potential therapeutic strategies. Development of novel anti-cancer drugs or new targeted strategies to conquer drug resistance is a key goal of cancer research. In this respect, novel tumor gatekeepers and innovative targeted strategies can be helpful in overcoming drug resistance as well as improve currently used targeted therapies. In this review, efforts have been made to present some of the latest knowledge about novel tumor gatekeepers and new therapeutic strategies to improve the efficacy of chemotherapy and give new hope to cancer patients to fight against cancer.
期刊：
Journal of Biological Chemistry,2017年292(7):2933-2943 ISSN：0021-9258
通讯作者：
Wu, Kongming;Liu, Kaiyu
作者机构：
[Hu, Ruqin; Xiao, Yutao; Wu, Kongming; Liang, Gemei] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, West Yuanmingyuan Rd, Beijing 100193, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Hu, Ruqin; Liu, Kaiyu; Yang, Yongbo; Dai, Qing] Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.;[Soberon, Mario; Pacheco, Sabino; Bravo, Alejandra] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
通讯机构：
[Wu, Kongming; Liu, Kaiyu] C;Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, West Yuanmingyuan Rd, Beijing 100193, Peoples R China.;Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.
摘要：
Transgenic plants that produce Bacillus thuringiensis (Bt) crystalline (Cry) toxins are cultivated worldwide to control insect pests. Resistance to B. thuringiensis toxins threatens this technology, and although different resistance mechanisms have been identified, some have not been completely elucidated. To gain new insights into these mechanisms, we performed multiple back-crossing from a 3000-fold Cry1Ac-resistant BtR strain from cotton bollworm (Helicoverpa armigera), isolating a 516-fold Cry1Ac-resistant strain (96CAD). Cry1Ac resistance in 96CAD was tightly linked to a mutant cadherin allele (mHaCad) that contained 35 amino acid substitutions compared with HaCad from a susceptible strain (96S). We observed significantly reduced levels of the mHaCad protein on the surface of the midgut epithelium in 96CAD as compared with 96S. Expression of both cadherin alleles from 96CAD and 96S in insect cells and immunofluorescence localization in insect midgut tissue sections showed that the HaCAD protein from 96S localizes on the cell membrane, whereas the mutant 96CAD-mHaCad was retained in the endoplasmic reticulum (ER). Mapping of the mutations identified a D172G substitution mainly responsible for cadherin mislocalization. Our finding of a mutation affecting membrane receptor trafficking represents an unusual and previously unrecognized B. thuringiensis resistance mechanism.
期刊：
Journal of Virology,2017年91(1):JVI.01831-16 ISSN：0022-538X
通讯作者：
Qiu, Jianming
作者机构：
[Xu, Peng; Shen, Weiran; Qiu, Jianming; Zou, Wei; Deng, Xuefeng] Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66103 USA.;[Xu, Peng; Liu, Kaiyu; Peng, Jianxin] Cent China Normal Univ, Coll Life Sci, Wuhan, Peoples R China.;[Engelhardt, John F.; Yan, Ziying] Univ Iowa, Dept Anat & Cell Biol, Iowa City, IA USA.
通讯机构：
[Qiu, Jianming] U;Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66103 USA.
关键词：
DNA damage;DNA replication;parvovirus
摘要：
Human bocavirus 1 (HBoV1), an emerging human-pathogenic respiratory virus, is a member of the genus Bocaparvovirus of the Parvoviridae family. In human airway epithelium air-liquid interface (HAE-ALI) cultures, HBoV1 infection initiates a DNA damage response (DDR), activating all three phosphatidylinositol 3-kinase-related kinases (PI3KKs): ATM, ATR, and DNA-PKcs. In this context, activation of PI3KKs is a requirement for amplification of the HBoV1 genome (X. Deng, Z. Yan, F. Cheng, J. F. Engelhardt, and J. Qiu, PLoS Pathog, 12:e1005399, 2016, https://doi.org/10.1371/journal.ppat.1005399), and HBoV1 replicates only in terminally differentiated, nondividing cells. This report builds on the previous discovery that the replication of HBoV1 DNA can also occur in dividing HEK293 cells, demonstrating that such replication is likewise dependent on a DDR. Transfection of HEK293 cells with the duplex DNA genome of HBoV1 induces hallmarks of DDR, including phosphorylation of H2AX and RPA32, as well as activation of all three PI3KKs. The large viral nonstructural protein NS1 is sufficient to induce the DDR and the activation of the three PI3KKs. Pharmacological inhibition or knockdown of any one of the PI3KKs significantly decreases both the replication of HBoV1 DNA and the downstream production of progeny virions. The DDR induced by the HBoV1 NS1 protein does not cause obvious damage to cellular DNA or arrest of the cell cycle. Notably, key DNA replication factors and major DNA repair DNA polymerases (polymerase eta [Pol eta] and polymerase kappa [Pol kappa]) are recruited to the viral DNA replication centers and facilitate HBoV1 DNA replication. Our study provides the first evidence of the DDR-dependent parvovirus DNA replication that occurs in dividing cells and is independent of cell cycle arrest. IMPORTANCE The parvovirus human bocavirus 1 (HBoV1) is an emerging respiratory virus that causes lower respiratory tract infections in young children worldwide. HEK293 cells are the only dividing cells tested that fully support the replication of the duplex genome of this virus and allow the production of progeny virions. In this study, we demonstrate that HBoV1 induces a DDR that plays significant roles in the replication of the viral DNA and the production of progeny virions in HEK293 cells. We also show that both cellular DNA replication factors and DNA repair DNA polymerases colocalize within centers of viral DNA replication and that Pol eta and Pol kappa play an important role in HBoV1 DNA replication. Whereas the DDR that leads to the replication of the DNA of other parvoviruses is facilitated by the cell cycle, the DDR triggered by HBoV1 DNA replication or NS1 is not. HBoV1 is the first parvovirus whose NS1 has been shown to be able to activate all three PI3KKs (ATM, ATR, and DNA-PKcs).
摘要：
Herein, we report the identification of putative promoters for the non-structural proteins (NS) and capsid structural proteins (VP) of Helicoverpa armigera densovirus (HaDV2) as well as a potential mechanism for how these promoters might be regulated. For the first time, we report that VP is able to transactivate the VP promoter and, to a lesser degree, the NS promoter in densoviruses. In addition to this, another promoter-like sequence designated P2, when co-transfected with the VP gene, enhanced luciferase activity by approximately 35 times compared to a control. This suggests that there are two promoters for VP in HaDV2 and that the VP of parvoviruses might play a more important role in viral transcription than previously appreciated.
作者机构：
[Xu, Peng; Liu, Kaiyu; Peng, Jianxin] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;[Xu, Peng; Qiu, Jianming; Zou, Wei; Deng, Xuefeng; Ganaie, Safder S.] Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66103 USA.;[Zhou, Zhe; Wang, Shengqi] Beijing Inst Radiat Med, Dept Biotechnol, Beijing, Peoples R China.;[Ye, Shui Qing; Xiong, Min] Childrens Mercy Hosp, Dept Pediat, Kansas City, MO 64108 USA.;[Ye, Shui Qing; Xiong, Min] Childrens Mercy Hosp, Dept Biomed & Hlth Informat, Kansas City, MO 64108 USA.
通讯机构：
[Liu, Kaiyu] C;[Qiu, Jianming] U;[Wang, Shengqi] B;Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66103 USA.
关键词：
Phosphorylation;Cell cycle and cell division;Cyclins;Flow cytometry;DNA replication;Gene expression;Synthesis phase;Transactivation
摘要：
Basic helix-loop-helix transcription factors (TFs), namely MYC2, MYC3, and MYC4, interact with Jasmonate Zim-domain proteins and are their direct targets. These TFs have been shown to function synergistically to control Arabidopsis growth and development. Our results showed similar MYC2, MYC3, and MYC4 expression patterns during Arabidopsis seed development, which remained relatively high during seed mid-maturation. MYC2, MYC3, and MYC4 acted redundantly in seed size, weight control, and in regulating seed storage protein accumulation. Triple mutants produced the largest seeds and single and double mutants' seeds were much larger than those of wild type. The weight of triple mutants' seeds was significantly higher than that of wild-type seeds, which was accompanied by an increase in seed storage protein contents. Triple mutants' seeds presented a marked decrease in 2S amounts relative to those in wild-type seeds. Liquid chromatography tandem mass spectra sequencing results indicated that both the relative abundance and the peptide number of CRA1 and CRU3 were greatly increased in triple mutants compared to wild type. The expression of 2S1-2S5 decreased and that of CRA1 and CRU3 increased in triple mutants relative to those in wild types during seed development, which might have contributed to the low 2S and high 12S contents in triple mutants. Our results contribute to understanding the function of MYC2, MYC3, and MYC4 on seed development, and provide promising targets for genetic manipulations of protein-producing crops to improve the quantity and quality of seed storage proteins. (C) 2016 Elsevier Masson SAS. All rights reserved.
摘要：
The CW domain is a zinc binding domain, composed of approximately 50- 60 amino acid residues with four conserved cysteine (C) and two to four conserved tryptophan (W) residues. The members of the superfamily of CW domain containing proteins, comprised of 12 different eukaryotic nuclear protein families, are extensively expressed in vertebrates, vertebrate-infecting parasites and higher plants, where they are often involved in chromatin remodeling, methylation recognition, epigenetic regulation and early embryonic development. Since the first CW domain structure was determined 5 years ago, structures of five CW domains have been solved so far. In this review, we will discuss these recent advances in understanding the identification, definition, structure, and functions of the CW domain containing proteins.
期刊：
CURRENT PROTEIN & PEPTIDE SCIENCE,2016年17(4):306-318 ISSN：1389-2037
通讯作者：
Liu, Ke;Qi, Chao
作者机构：
[Liu, Ke; Qi, Chao; Liu, Jinlin; Ye, Weiyuan; Ding, Yumin; Liu, Yanli; Yang, Jihong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, K; Qi, C] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
m6A;RNA methyltransferase and demethylase;YTH domain.
摘要：
RNA modification, involving in a wide variety of cellular processes, has been identified over 100 types since 1950s. N6-methyladenosine (m6A), as one of the most abundant RNA modifications, is found in several RNA species and predominantly located in the stop codons, long internal exons as well as 3’UTR. It was reported that m6A modification preferentially appears after G in the conserved motif RRm6ACH (R = A/G and H = A/C/U). There are two families of enzymes responsible for maintaining the balance of m6A modification: m6A methyltransferases and demethylases, which add and remove methyl marks for adenosine of RNA, respectively. METTL3 complex, the m6A methyltransferases, and two kinds of demethylases including Fat mass and obesity-associated protein (FTO) and alkylation protein AlkB homolog 5 (ALKBH5) are characterized thus far. Besides the “writers” and “erasers”, m6A specific recognizing proteins, such as the YTH (YT521-B homology) domain family proteins, also have attracted significant attention. Herein, we focus on the recent progress in understanding the biological/biochemical functions and structures of proteins responsible for the m6A modification and recognition. Detailed analyses of these important proteins are essential for the further study of their biological function and will also guide us in designing more potent and specific small-molecule chemical inhibitors for these targets.
