作者机构：
[Wan, Cuihong; Peng, Zhao] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Peng, Zhao] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Li, Jiaqiang; Jiang, Xingpeng] Cent China Normal Univ, Sch Comp, Wuhan 430079, Hubei, Peoples R China.;[Li, Jiaqiang; Jiang, Xingpeng] Cent China Normal Univ, Hubei Prov Key Lab Artificial Intelligence & Smart, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Xingpeng Jiang; Cuihong Wan] S;School of Computer, and Hubei Provincial Key Laboratory of Artificial Intelligence and Smart Learning, Central China Normal University , Wuhan 430079, Hubei , People's Republic of China<&wdkj&>School of Life Sciences, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079, Hubei , People's Republic of China
摘要：
As one of the essential life forms in the biosphere, research on cyanobacteria has been growing remarkably for decades. Biological functions in organisms are often accomplished through protein-protein interactions (PPIs), which help to regulate interacting proteins or organize them into an integral machine. However, the study of PPIs in cyanobacteria falls far behind that in mammals and has not been integrated for ease of use. Thus, we built CyanoMapDB (http://www.cyanomapdb.msbio.pro/), a database providing cyanobacterial PPIs with experimental evidence, consisting of 52,304 PPIs among 6,789 proteins from 23 cyanobacterial species. We collected available data in UniProt, STRING, and IntAct, and mined numerous PPIs from co-fractionation MS data in cyanobacteria. The integrated data are accessible in CyanoMapDB (http://www.cyanomapdb.msbio.pro/), enabling users to easily query proteins of interest, investigate interacting proteins with evidence from different sources, and acquire a visual network of the target protein. We believe that CyanoMapDB will promote research involved with cyanobacteria and plants.
作者机构：
[Zhang, Liang; Sun, Hang; Deng, Tao; Chen, Jun-Tong; Sun, H; Deng, T; Huang, Xian-Han; Zhang, Xin-Jian; Kuang, Tian-Hui] Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China.;[Chen, Jun-Tong; Zhang, Xin-Jian; Kuang, Tian-Hui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Liden, Magnus] Uppsala Univ, Evolutionary Biol Ctr, Systemat Biol, S-75236 Uppsala, Sweden.;[Landis, Jacob B.] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Biol, Ithaca, NY 14853 USA.;[Landis, Jacob B.] Cornell Univ, LH Bailey Hortorium, Ithaca, NY 14853 USA.
通讯机构：
[Sun, H ; Deng, T] C;Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China.
关键词：
character evolution;Corydalis;genome skimming;Papaveraceae;phylogeny
摘要：
Phylogenetic analyses of Corydalis and related taxa, covering all 42 previously recognized sections and five independent “series”, combined with ancestral character reconstructions, uncovered previously unrecognized relationships and greatly improved our understanding of the evolution of the genus Corydalis. Abstract The genus Corydalis, with ca. 530 species, has long been considered taxonomically challenging because of its great variability. Previous molecular analyses, based on a few molecular markers and incomplete taxonomic sampling, were clearly inadequate to delimit sections and subgenera. We have performed phylogenetic analyses of Corydalis and related taxa, using 65 shared protein‐coding plastid genes from 313 accessions (including 280 samples of ca. 226 species of Corydalis) and 152 universal low‐copy nuclear genes from 296 accessions (including 271 samples of Corydalis) covering all 42 previously recognized sections and five independent “series”. Phylogenetic trees were inferred using Bayesian Inference and Maximum Likelihood. Eight selected morphological characters were estimated using ancestral state reconstructions. Results include: (i) of the three subgenera of Corydalis, two are fully supported by both the plastid and nuclear data; the third, subg. Cremnocapnos, is weakly supported by plastid DNA only, whereas in the nuclear data the two included sections form successive outgroups to the rest of the genus; (ii) among all 42 sections and five “series”, 25 sections and one “series” are resolved as monophyletic in both data sets; (iii) the common ancestor of Corydalis is likely to be a perennial plant with a taproot, yellow flowers with a short saccate spur, linear fruits with recurved fruiting pedicels, and seeds with elaiosomes; (iv) we provide a new classification of Corydalis with four subgenera (of which subg. Bipapillatae is here newly described), 39 sections, 16 of which are consistent with the previous classification, 16 sections have been recircumscribed, one section has been reinstated and six new sections are established. Characters associated with lifespan, underground structures, floral spur, fruit and elaiosomes are important for the recognition of subgenera and sections. These new phylogenetic analyses combined with ancestral character reconstructions uncovered previously unrecognized relationships, and greatly improved our understanding of the evolution of the genus.
摘要：
Predator-prey interactions are important but difficult to study in the field. Therefore, laboratory studies are often used to examine the outcomes of predator-prey interactions. Previous laboratory studies have shown that moth hearing and ultrasound production can help prey avoid being eaten by bats. We report here that laboratory behavioural outcomes may not accurately reflect the outcomes of field bat-moth interactions. We tested the success rates of two bat species capturing moths with distinct anti-bat tactics using behavioural experiments. We compared the results with the dietary composition of field bats using next-generation DNA sequencing. Rhinolophus episcopus and Rhinolophus osgoodi had a lower rate of capture success when hunting for moths that produce anti-bat clicks than for silent eared moths and earless moths. Unexpectedly, the success rates of the bats capturing silent eared moths and earless moths did not differ significantly from each other. However, the field bats had a higher proportion of silent eared moths than that of earless moths and that of clicking moths in their diets. The difference between the proportions of silent eared moths and earless moths in the bat diets can be explained by the difference between their abundance in bat foraging habitats. These findings suggest that moth defensive tactics, bat countertactics and moth availability collectively shape the diets of insectivorous bats. This study illustrates the importance of using a combination of behavioural experiments and molecular genetic techniques to reveal the complex interactions between predators and prey in nature.
作者机构：
[Dai, Xiongfeng; Zhu, Manlu; Dai, XF] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Zhu, ML; Dai, XF ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
摘要：
Timely adaptation to nutrient downshift is crucial for bacteria to maintain fitness during feast and famine cycle in the natural niche. However, the molecular mechanism that ensures the timely adaption of bacterial growth to nutrient downshift remains poorly understood. Here, we quantitatively investigated the adaptation of Escherichia coli to various kinds of nutrient downshift. We found that relA deficient strain, which is devoid of stringent response, exhibits a significantly longer growth lag than wild type strain during adapting to both amino acid downshift and carbon downshift. Quantitative proteomics show that increased (p)ppGpp level promotes the growth adaption of bacteria to amino acid downshift via triggering the proteome resource re-allocation from ribosome synthesis to amino acid biosynthesis. Such type of proteome re-allocation is significantly delayed in the relA-deficient strain, which underlies its longer lag than wild type strain during amino acid downshift. During carbon downshift, a lack of stringent response in relA deficient strain leads to disruption of the transcription-translation coordination, thus compromising the transcription processivity and further the timely expression of related catabolic operons for utilizing secondary carbon sources. Our studies shed light on the fundamental strategy of bacteria to maintain fitness under nutrient-fluctuating environments. Bacteria undergo nutrient fluctuations during repeated feast and famine cycles and need to metabolically adapt to these changes. Using quantitative proteomics, Zhu & Dai show that the stringent response of (p)ppGpp is crucial for the timely adaption of bacterial growth to both amino acid and carbon downshift.
期刊：
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION,2023年107(1):228-237 ISSN：0931-2439
通讯作者：
Wan, LL;Ai, H
作者机构：
[Feng, Ke; Chen, Yan; Lu, Jianjian] Wuhan Polytech Univ, Sch Mech Engn, Wuhan, Peoples R China.;[Wan, Lili] Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Peoples R China.;[Ai, Hui] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.
通讯机构：
[Ai, H ] C;[Wan, LL ] W;Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.
摘要：
The chicken is rich in various proteins, fatty acids, polysaccharides, trace elements, and other human essential nutrients that contribute to its high nutritional value. In this study, the expression levels of nutrition-related genes (acetyl-CoA acyltransferase, ACAA) of native chicken breeds were investigated. The level of GgalACAA1-2 transcripts expression in the liver of chicken was significantly higher than that of muscle and heart. Moreover, three protein extracts were isolated from the muscle, heart, and liver tissues from the chicken, and their nutritional function was evaluated in the present study. These protein extracts had excellent DPPH and hydroxyl radical scavenging capacities and exhibited significant superoxide anion scavenging ability. Moreover, the protein extracts of muscle tissue showed an important mouse splenocyte proliferation activity and could be used as an immunomodulator of natural origin. In addition, this report presented an automatic visual inspection of chicken viscera using the active contour algorithms and the image processing method for eviscerating by the parallel robot. The recognition and positioning rate of chicken viscera obtained by the proposed method could reach 96.45%. These methods provided basic data for automated poultry slaughter and segmentation, avoiding unnecessary health risks by a pathogenic microorganism, such as avian influenza, Newcastle disease virus, and coronavirus. Moreover, the internal organs of the chicken could be fully harvested by the image segmentation of automatic evisceration, which also facilitated the processing value of these internal organs as by-products of poultry.
期刊：
JOURNAL OF PROTEOME RESEARCH,2023年22(4):1172-1180 ISSN：1535-3893
通讯作者：
Cuihong Wan
作者机构：
[Wang, Yi; Wan, Cuihong; Zhang, Zheng; Peng, Mingbo; Pan, Ni] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wang, Yi; Wan, Cuihong; Zhang, Zheng; Peng, Mingbo; Pan, Ni] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Tian, Tao; Pan, Zhenwei; Zhao, Xinbo] Minist Educ, Dept Pharmacol, Key Lab Cardiovasc Med Res, Harbin 150081, Heilongjiang, Peoples R China.;[Tian, Tao; Pan, Zhenwei; Zhao, Xinbo] Harbin Med Univ, State Prov Key Labs Biomed Pharmaceut China, Harbin 150081, Heilongjiang, Peoples R China.
通讯机构：
[Cuihong Wan] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People’s Republic of China
摘要：
The incidence rate of atrial fibrillation (AF) has stayed at a high level in recent years. Despite the intensive efforts to study the pathologic changes of AF, the molecular mechanism of disease development remains unclarified. Microproteins are ribosomally translated gene products from small open reading frames (sORFs) and are found to play crucial biological functions, while remain rare attention and indistinct in AF study. In this work, we recruited 65 AF patients and 65 healthy subjects for microproteomic profiling. By differential analysis and cross-validation between independent datasets, a total of 4 microproteins were identified as significantly different, including 3 annotated ones and 1 novel one. Additionally, we established a diagnostic model with either microproteins or global proteins by machine learning methods and found the model with microproteins achieved comparable and excellent performance as that with global proteins. Our results confirmed the abnormal expression of microproteins in AF and may provide new perspectives on the mechanism study of AF.
作者机构：
[Shuang-Quan Huang; Meng Zhang; Ze-Yu Tong; Ling-Yun Wu; Hui-Hui Feng] Institute of Evolution and Ecology,School of Life Sciences,Central China Normal University,China;[Ling-Yun Wu] Faculty of Resources and Environmental Science,Hubei University,China;[W.Scott Armbruster] School of Biological Sciences,University of Portsmouth,UK;[W.Scott Armbruster] Institute of Arctic Biology,University of Alaska Fairbanks,USA;[Susanne S.Renner] Department of Biology,Washington University,USA
通讯机构：
[Susanne S Renner] D;[Shuang-Quan Huang] I;Department of Biology, Washington University , USA<&wdkj&>Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University , China
摘要：
The services of animal pollinators that deliver compatible pollen to receptive stigmas are essential for the reproduc-tion of many flowering plants[1,2].The maintenance of plant-pollinator interac-tions,therefore,is the basis for the func-tion and sustainability of healthy ecosys-tems.For this reason,there has been widespread concern over the past two decades about the global decline in pol-linators and the effects of this loss on pollination services for both wild plants and crops[3,4].
作者机构：
[Qi, Yong; Wang, Shan-Shan; Li, Lu-Lin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
通讯机构：
[Lu-Lin Li] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China
关键词：
AcMNPV;IE1;baculovirus;late gene expression
摘要：
The late gene expression of baculovirus has been known to depend on the virus-encoded RNA polymerase, which consists of four subunits. The immediate-early gene ie1 was found to be required for viral early gene expression, late gene expression, and DNA replication. Early and late gene expressions of baculoviruses have been known to rely on host RNA polymerase II and a virus-encoded RNA polymerase, separately. In this study, we found that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) recombinant bacmids with the individual RNA polymerase subunit genes deleted could support low levels of expression of a reporter gene under the control of the promoter of a typical late gene, vp39, in transfected Sf9 cells. Through multistep subcloning of a genomic library of the virus and transient expression assay analysis, ie1 was identified to be the only viral gene that was responsible for activation of late gene expression in the absence of the viral RNA polymerase. Furthermore, IE1 was found to be capable of activating reporter gene expression from the promoters of additional late genes polh, p6.9, odv-e18, odv-e25, and gp41, independent of any additional viral factors. Deletion of ie1 from the virus genome eliminated late gene expression. The IE1-activated late gene expression was enhanced by the viral hr4b. It was shown to be insensitive to inhibition of alpha-amanitin and did not appear to have stable transcription start sites. It is proposed that IE1 may serve to recruit newly synthesized viral RNA polymerase to viral DNA by activating low levels of pretranscription of the late genes to create an appropriate DNA conformation.IMPORTANCE The late gene expression of baculovirus has been known to depend on the virus-encoded RNA polymerase, which consists of four subunits. The immediate-early gene ie1 was found to be required for viral early gene expression, late gene expression, and DNA replication. How it functions in late gene expression remains unclear. In this study, we found that AcMNPV IE1 could activate low levels of gene expression from late gene promoters independently of any additional viral factors, with nonspecific transcription start sites. This new finding will shed light on the role of IE1 in the regulation of late gene expression and the understanding of the mechanism of late gene transcription initiation.
期刊：
Journal of Ethnopharmacology,2023年316:116723 ISSN：0378-8741
通讯作者：
Wei Yang<&wdkj&>Lei Liu
作者机构：
[Sun, Ying; Li, Jingyi; Wang, Guannan; Zheng, Lihua; Pei, Yiying; Yang, Qifang] National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China;[Zhang, Ming] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China;[Yang, Wei] Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, Second Hospital of Jilin University, Changchun, China. Electronic address: wyang2002@jlu.edu.cn;[Liu, Lei] National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China. Electronic address: liul905@nenu.edu.cn
通讯机构：
[Wei Yang] J;[Lei Liu] N;National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China<&wdkj&>Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, Second Hospital of Jilin University, Changchun, China
关键词：
Alkaloid;Antidepressant;BDNF;Sophora Alopecuroides L.
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Depression has become a global public health problem and the development of new highly effective, low-toxicity antidepressants is imminent. Sophora alopecuroides L. is a common medicinal plant, which has therapeutic effect on central nervous system diseases. AIM OF THE STUDY: In this study, the antidepressant effect of total alkaloids (ALK) isolated from Sophora alopecuroides L. was explored and the mechanism was further elucidated. MATERIALS AND METHODS: A primary neuronal injury model was established in vitro by corticosterone. ICR mice were then selected to construct an in vivo model of chronic unpredictable mild stress (CUMS)-induced depression, and the ameliorative effects of ALK on depression were examined by various behavioral tests. The antidepressant molecular mechanism of ALK was subsequently revealed by ELISA, Western blot, immunohistochemistry and Golgi staining. RESULTS: BDNF secretion as well as TrkB and ERK phosphorylated protein levels were found to be improved in primary cortical neurons, along with improved dendritic complexity of neurons. The results of in vivo showed that the depression-like behavior of CUMS-induced mice was reversed after 2 weeks of continuous gavage administration of ALK, and the neurotransmitter levels in the plasma of mice were increased. Moreover, the expression levels of key proteins of BDNF-AKT-mTOR pathway and the complexity of neuronal dendrites were improved in the prefrontal cortex of mice. CONCLUSIONS: These findings indicate that ALK of Sophora alopecuroides L. can effectively improve the depressive phenotype of mice, possibly by promoting the expression of BDNF in prefrontal cortex, activating the downstream AKT/mTOR signal pathway, and ultimately enhancing neuronal dendritic complexity.
摘要：
Studies have shown that exposure to either microplastics (MPs) or di-(2-ethylhexyl) phthalic acid (DEHP) alone can cause neurotoxicity in animals, but it remains uncertain whether and to what extent co-exposure to these two substances, which often occur together in reality, can also induce neurotoxicity. This study aimed to investigate the neurotoxicity and molecular mechanisms of combined exposure to DEHP and polypropylene microplastics (synthetic PP-MPs were used), the microplastics most commonly encountered by young children, in immature mice. The results showed that exposure to PP-MPs and/or DEHP did cause neurotoxic effects in immature mice, including induction of neurocognitive and memory deficits, damage to the CA3 region of the hippocampus, increased oxidative stress, and decreased AChE activity in the brain. The severity of the neurotoxicity increased with increasing concentrations of PP-MPs, combined exposure to PP-MPs and DEHP exhibited additive or synergistic effects. Transcriptomic analyses revealed that the PP-MPs and/or DEHP exposure altered the expression profiles of gene clusters involved in the stress response, and in protein processing in endoplasmic reticulum. Quantitative analyses further indicated that PP-MPs and/or DEHP exposure inhibited the activity of the heat shock response mediated by heat shock transcription factor 1, while chronically activated the unfolded protein response, consequently inducing neurotoxicity through neuronal apoptosis and neuroinflammation in the immature mice. As a pioneer study to highlight the neurotoxicity induced by combined exposure to PP-MPs and DEHP in immature mice, this research provides new insights into mitigating the health risks of PP-MPs and DEHP exposure in young children.
期刊：
Landscape and Urban Planning,2023年233:104712 ISSN：0169-2046
通讯作者：
Hongyu Niu<&wdkj&>Hongmao Zhang
作者机构：
[Yue, Xingcheng; Chen, Zhiwen; Niu, Hongyu; Zhang, Hongmao; Zhao, Hengyue] Cent China Normal Univ, Inst Ecol & Evolut, Sch Life Sci, Wuhan 430079, Peoples R China.;[Rehling, Finn; Schabo, Dana G.; Niu, Hongyu; Farwig, Nina] Philipps Univ Marburg, Dept Biol, Conservat Ecol, D-35043 Marburg, Germany.;[Wang, Xiaorong] Hubei Acad Forestry, Wuhan 430075, Peoples R China.;[Niu, Hongyu; Zhang, Hongmao] 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Hongyu Niu; Hongmao Zhang] I;Institute of Ecology and Evolution, School of Life Sciences, Central China Normal University, Wuhan 430079, China<&wdkj&>Conservation Ecology, Department of Biology, Philipps-University of Marburg, Marburg 35043, Germany<&wdkj&>Institute of Ecology and Evolution, School of Life Sciences, Central China Normal University, Wuhan 430079, China
摘要：
Urban forests are highly fragmented in mega-cites, acting as islands in terms of preserving species diversity. To maintain the ecological services of urban forests, management measures such as reforestation have been implemented, which might have a long-term effect on biodiversity. To understand how fragmentation and reforestation affect the natural regeneration of urban forests, we investigated the relationship between forest area, isolation and seed dispersal mode and the diversity and composition of woody species at the tree, seedling, and sapling stages in 28 secondary forests of the mega-city of Wuhan, China. We found that the alpha diversity of woody species was positively correlated with forest area, while their beta diversity was negatively correlated with forest area. The beta diversity of nonanimal-dispersed species significantly correlated with isolation. Animal-dispersed plants had consistently a higher alpha diversity from trees to seedlings and saplings, while their beta diversity was lower than nonanimal-dispersed plants at the seedling and sapling stage. The community composition of woody plants in urban forests was largely congruent among the three life stages. However, only the communities of animal-dispersed plants were consistent across life stages in small or highly-isolated forest patches. The results show that the woody plant diversity of urban forests is largely similar to that expected by island theory. Animal-dispersed trees are more likely to regenerate successfully due to a more diverse set of species used in reforestation and their higher tolerance to urban forest fragmentation. More management measures for nonanimal-dispersed species, such as enriching and repeating reforestation, will be required to maintain their high biodiversity in urban forests.
