作者机构：
[Feng, Guoqiang; Gong, Shengyi; Gui, Zhisheng] Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
摘要：
Dual-state emissive (DSE) materials exhibit fluorescence in both solid and solution states and have become an emerging material in the fields of materials science and sensing in recent years. However, due to the lack of effective and universal preparation methods, DSE materials, especially those with long emission wavelengths, are still scarce. Developing an effective method for constructing such DSE molecules is urgently needed. In this study, we constructed three DSE molecules (NRP-Boc, DCIP-Boc, and DCMP-Boc) with far-red to near-infrared fluorescence by simply modifying three traditional aggregation-caused quenching (ACQ) fluorophores with tert-butyloxycarbonyl (Boc) groups. Density functional theory (DFT) calculations and crystal data revealed the reasons for the bright fluorescence of these three molecules in solution and solid, demonstrating that this Boc protection method is a simple and effective strategy for constructing DSE molecules. We also found that these three DSE molecules have the potential to target and visualize lipid droplets (LDs). Among them, DCIP-Boc shows advantages of a large Stokes shift, long emission wavelength, low fluorescence background, and good photostability in cells, providing a powerful new molecular tool with DSE property for high-fidelity imaging of LDs.
期刊：
Sensors and Actuators B-Chemical,2024年406:135432 ISSN：0925-4005
通讯作者：
Feng, GQ
作者机构：
[Zheng, Zhoupeng; Feng, Guoqiang; Feng, GQ; Zhang, Jinzheng; Liu, Yijia; Gong, Shenyi] Cent China Normal Univ, Coll Chem, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, GQ ] C;Cent China Normal Univ, Coll Chem, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
Dual -channel fluorescent probe;Viscosity;Peroxynitrite;Mitochondria;Diabetes;In vivo imaging
摘要：
Diabetes is a common metabolic disease worldwide, which often has serious complications and seriously threatens human health. Abnormal viscosity and peroxynitrite (ONOO-) concentration are closely related to diabetes. Herein, we report a fluorescent dual response probe AO, which can simultaneously detect the changes of viscosity and ONOO- in diabetes at dual fluorescence channels. AO sensitively responds to viscosity and ONOO- with significant emission signal changes at 710 nm and 645 nm, respectively. Moreover, AO selectively targets mitochondria and generates a new compound when detecting ONOO-, which can spontaneously target and light up another type of organelle (lipid droplets), thus showing spatially separated dual -channel fluorescence signals. More notably, AO was successfully used to simultaneously detect the viscosity and ONOO- variations in hyperglycemic cells, diabetes and its complications (using mice models) for the first time, which may lead to a better understanding of the physiological and pathological phenomena of diabetes.
作者机构：
[Feng, Guoqiang; Li, Qianhua; Gong, Shengyi; Jiang, Siyu; Zhu, Wenlong] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
摘要：
LDs (Lipid droplets) are key organelles for lipid metabolism and storage, which are closely related to ferroptosis and fatty liver. Due to its small size and highly dynamic nature, developing high-fidelity fluorescent probes for imaging of LDs is crucial for observing the dynamic physiological processes of LDs and investigating LDs-associated diseases. Herein, we synthesized three dicyanoisophorone-based fluorescent probes (DCIMe, DCIJ, and DCIQ) with different electron-donating groups and studied their imaging performance for LDs. The results show that DCIQ is highly polarity sensitive and can perform high-fidelity imaging for LDs, with significantly better performance than DCIMe, DCIJ, and commercial LD probe BODIPY 493/503. Based on this, DCIQ was successfully applied to real-time observe the interplays between LDs and other organelles (mitochondria, lysosomes, and endoplasmic reticulum), and to image the dynamics of LDs with fast scanning mode (0.44s/frame) and the generation of oleic acid-induced LDs with high-fidelity. Finally, DCIQ was used to study the changes of LDs in the ferroptosis process and nonalcoholic fatty liver disease tissues. Overall, this study provided a powerful tool for high-fidelity imaging of LDs in cells and tissues.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
作者机构：
[Feng, Guoqiang; Li, Qianhua; Gong, Shengyi; Feng, GQ; Zhu, Wenlong] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, GQ ] C;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
Cancer cells;Cell membrane;Early tumor diagnosis;NIR fluorescent probe;Polarity
摘要：
The development of a sensitive method for early cancer diagnosis is very important because the early diagnosis of cancer is crucial in preventing the spread of cancer cells and improving patient survival rates. Recent studies showed that cancer cell membranes have lower polarity than normal cell membranes, which provides a new approach for cancer diagnosis at the cell membrane level. We developed herein a highly sensitive cell membrane polarity probe (<bold>Cal-M</bold>) for early diagnosis of cancer. This probe has low cytotoxicity, good photostability, near-infrared (NIR) fluorescence emission (>700 nm), large Stokes shift, high sensitivity for polarity, excellent cell membrane localization performance, and the ability to selectively light up cancer cells. Using this probe staining, the fluorescence of cancer cells is & SIM;63 times higher than that of normal cells, demonstrating excellent sensitivity and selectivity of <bold>Cal-M</bold>. This probe was also successfully used to detect polarity changes on cancer cell membranes and selectively visualize tumors in mice. Notably, the tumor could be visualized sensitively with a size as small as 1.37 mm(3), indicating that <bold>Cal-M</bold> is promising for early diagnosis of tumors.
作者机构：
[Feng, Guoqiang; Hong, Jiaxin; Li, Qianhua; Zhang, Jinzheng] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Ministr Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
作者机构：
[Feng, Guoqiang; Hong, Jiaxin; Gong, Shengyi; Li, Qianhua; Feng, GQ; Jiang, Siyu] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent BiosensorTechnol & H, Natl Key Lab Green Pesticide, Green Pesticide, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, GQ ] C;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent BiosensorTechnol & H, Natl Key Lab Green Pesticide, Green Pesticide, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
摘要：
With the widespread use of drugs, drug-induced acute kidney injury (AKI) has become an increasingly serious health concern worldwide. Currently, early diagnosis of drug-induced AKI remains challenging because of the lack of effective biomarkers and noninvasive imaging tools. SO(2) plays important physiological roles in living systems and is an important antioxidant for maintaining redox homeostasis. However, the relationship between SO(2) (in water as SO(3)(2-)/HSO(3)(-)) and drug-induced AKI remains largely unknown. Herein, we report the highly sensitive near-infrared fluorescence probe DSMN, which for the first time reveals the relationship between SO(2) and drug-induced AKI. The probe responds to SO(3)(2-)/HSO(3)(-) selectively and rapidly (within seconds) and shows a significant turn-on fluorescence at 710 nm with a large Stokes shift (125 nm). With these properties, the probe was successfully applied to detect SO(2) in living cells and mice. Importantly, the probe can selectively target the kidneys, allowing for the detection of changes in the SO(2) concentration in the kidneys. Based on this, DSMN was successfully used to detect cisplatin-induced AKI and revealed an increase in the SO(2) levels. The results indicate that SO(2) is a new biomarker for AKI and that DSMN is a powerful tool for studying and diagnosing drug-induced AKI.
摘要：
Nitric oxide (NO) regulates cell damage by neurotoxin and participates in neuronal cell death in Parkinson's disease (PD). A large number of nitric oxide synthases involved in the synthesis of NO exists in the Golgi apparatus and hence, exploring the Golgi NO in PD is of great significance. Fluorescent probes provide powerful non-invasive detection and imaging tools in life systems. However, Golgi-targetable fluorescent probe has not yet been reported to detect and disclose the level changes of Golgi NO in PD. Herein, we report a Golgi-targetable turn-on fluorescence probe Gol-NO for the detection of Golgi NO, which shows low cytotoxicity, high sensitivity (about 1.6 nM of detection limit) and selectivity, and rapid response (<1 min) for NO. Moreover, we show that it can detect not only endogenous and exogenous NO in cells and zebrafish but also the level changes of NO in the rotenone-induced cell and zebrafish PD models, indicating that Gol-NO is a valuable new tool to explore NO in Golgi and study PD.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, PR China
作者机构：
[Feng, Guoqiang; Hong, Jiaxin; Li, Qianhua; Gong, Shengyi; Feng, Shumin] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
作者机构：
[Feng, Huili; Zhang, Xiangqian; Deng, Ganzhen] Huazhong Agr Univ, Coll Anim Sci & Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.;[Feng, Guoqiang; Tu, Le; Zhang, Yi; Guan, Xiaofang; Mei, Longcan; Sun, Yao; Li, Chonglu] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Minist Educ,Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.;[Li, Jie] Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China.;[Tu, Le] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Peoples R China.
通讯机构：
[Yao Sun] K;Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079 China
关键词：
cancer therapy;mitochondria-targeted;NIR-II region;organic nanosonosensitizer;sonodynamic therapy
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
摘要：
Both glutathione (GSH) and viscosity play an important role in mitochondria. They are closely related to various physiological and pathological processes and are important biomarkers in cancer analysis. Herein, we developed the first dual responsive fluorescence probe MGV that can simultaneously detect mitochondrial GSH and vis-cosity. MGV is smart and shows a selective ratiometric response to GSH at 535/650 nm with a significant in -crease of green fluorescence. MGV can also show a distinct red fluorescence enhancement at 627 nm as viscosity increases. In addition, MGV has low cytotoxicity and good mitochondrial-targeting ability. With these features, MGV was successfully applied to image mitochondrial GSH at dual fluorescence channels and track mitochon-drial viscosity changes on the red fluorescence channel. With MGV, the formation of mitochondrial bleb vesicles was observed with nystatin stimulation, and during the Cisplatin-induced apoptosis, both the level of GSH and the viscosity showed an increase. Finally, based on the dual-response to GSH and viscosity, MGV was successfully used for dual-channel imaging of cancer cells/tumors. Overall, this work provided a smart probe for GSH and viscosity and new insights/methods for apoptosis and tumor imaging.
作者机构：
[Feng, Guoqiang; Gong, Shengyi; Liu, Yijia; Feng, Shumin] Cent China Normal Univ, Coll Chem, Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
期刊：
Sensors and Actuators B-Chemical,2022年369:132325 ISSN：0925-4005
通讯作者：
Guoqiang Feng
作者机构：
[Feng, Guoqiang; Hong, Jiaxin; Li, Qianhua; Guan, Xiaogang] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Feng, Guoqiang] Cent China Normal Univ, Coll Chem, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
摘要：
Lysosome is one of the most crucial organelles in living systems. Lysosomal viscosity is an important microenvironment parameter in lysosomes, which is closely related to the occurrence and development of many diseases, including cancer. To monitor the highly dynamic lysosomes and the lysosomal viscosity changes, we developed a versatile high-performance fluorescent probe DCMP in this work. DCMP shows stronger fluorescence under acidic conditions or in solutions with higher viscosity, especially with high sensitivity to the change of viscosity. More importantly, DCMP can use a low dose (100 nM) and can target lysosomes and image lysosomes with high signal-to-noise ratio and high fidelity. It can also track the lysosomal motility and viscosity changes in real-time, and effectively monitor the interaction between lysosomes and damaged organelles. We also found that DCMP can be successfully used to selectively and sensitively light up cancer cells and cancer tissues. All the results show that this new probe has great potential not only in lysosomal high-fidelity imaging, but also in cancer detection.
期刊：
Sensors and Actuators B-Chemical,2021年326:129016 ISSN：0925-4005
通讯作者：
Feng, Guoqiang
作者机构：
[Feng, Guoqiang; Hong, Jiaxin] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
Cysteine;Isothiocyanate;Fluorescent probe;Bioimaging;Living cells and zebrafish
摘要：
Cysteine (Cys) is an essential biothiol and amino acid molecule, playing important roles in many physiological and pathological events. As a biomarker of many diseases, Cys has important clinical significance. Accordingly, it is of great significance to develop an efficient and specific detecting method for Cys in life systems. We report herein a new approach that isothiocyanate (ITC) can be used as an effective new specific recognition site of Cys to develop effective probes for the detection of Cys in life systems. To demonstrate the effectiveness of this approach, ITC was attached to an easy-to-prepare coumarin fluorophore and a novel molecular probe (CM-NCS) was synthesized. As expected, the probe could respond rapidly, selectively, and sensitively to Cys and could be well used for the detection of Cys in live cells and zebrafish. All the results demonstrated that the new recognition site (ITC) would provide a good new choice to develop highly Cys-selective probes.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
摘要：
Carbon monoxide (CO) plays a vital role in living organisms. The detection of CO at the subcellular level is of great significance to clarify its biological functions. Increasing evidence shows that CO is important in Golgi apparatus, but so far, no probe has been reported to detect CO in Golgi. In this paper, an effective fluorescence probe Gol-CO was reported for CO imaging in Golgi apparatus. Gol-CO uses a Golgi-targetable aminoquinoline derivative as the fluorophore and importantly, it shows significant ratiometric fluorescence signal changes at 520/425 nm in aqueous solutions with high selectivity and sensitivity (about 41 nM of detection limit) for CO. Imaging experiments showed that Gol-CO has excellent targeting ability to Golgi apparatus and excellent ratiometric fluorescence imaging ability for CO in cells and zebrafish. Gol-CO also succeeded in visualizing the level increase of CO in living cells under oxidative stress.
