期刊：
Sensors and Actuators B-Chemical,2021年326:129016 ISSN：0925-4005
通讯作者：
Feng, Guoqiang
作者机构：
[Feng, Guoqiang; Hong, Jiaxin] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
Cysteine;Isothiocyanate;Fluorescent probe;Bioimaging;Living cells and zebrafish
摘要：
Cysteine (Cys) is an essential biothiol and amino acid molecule, playing important roles in many physiological and pathological events. As a biomarker of many diseases, Cys has important clinical significance. Accordingly, it is of great significance to develop an efficient and specific detecting method for Cys in life systems. We report herein a new approach that isothiocyanate (ITC) can be used as an effective new specific recognition site of Cys to develop effective probes for the detection of Cys in life systems. To demonstrate the effectiveness of this approach, ITC was attached to an easy-to-prepare coumarin fluorophore and a novel molecular probe (CM-NCS) was synthesized. As expected, the probe could respond rapidly, selectively, and sensitively to Cys and could be well used for the detection of Cys in live cells and zebrafish. All the results demonstrated that the new recognition site (ITC) would provide a good new choice to develop highly Cys-selective probes.
作者机构：
[Feng, Guoqiang; Gong, Shengyi; Xia, Qingfeng; Zhou, Enbo] Cent China Normal Univ CCNU, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, G.] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, 152 Luoyu Road, China
关键词：
fluorescent probe;CO-releasing molecule;CORM-3;near-infrared fluorescence;living cell;in vivo imaging
摘要：
As a water-soluble carbon monoxide-releasing molecule, CORM-3 is widely used as a CO donor to study CO in the life system. CORM-3 can also replace gaseous CO as a therapeutic drug molecule to reveal the physiological and pathological effects of CO in life. Therefore, it is of great importance to visualize and track CORM-3 in the life system. We develop herein a near-infrared (NIR) fluorescent probe CORM3-NIR that can detect CORM-3 both in living cells and in vivo effectively. The probe is based on the unique fluorescent QCy7 and uses a 4-nitrobenzyl group to trap CORM-3, and importantly, it shows good water solubility and responds rapidly, selectively, and sensitively to CORM-3, releasing QCy-7 and producing distinct colorimetric and significant NIR fluorescence change signals at 743 nm. The Stokes shift is up to 81 nm. The probe is also able to detect CORM-3 ratiometrically with fluorescence at 743 and 600 nm. Besides, with low cytotoxicity, the probe also shows good NIR fluorescence bioimaging ability for CORM-3 in live cells and mice, which indicates that CORM3-NIR is an effective probe for tracking and studying CORM-3 in the life system.
摘要：
With a hybrid coumarin-dicyanoisophorone as report unit and dimethylthiocarbamate as response site, a novel reaction-based fluorescence probe (CDCI-HClO) was synthesized herein for rapid detection of hypochlorous acid (HOCl). CDCI-HClO can respond to HOCl quickly (almost in seconds), selectively, and sensitively, and give an obviously enhanced signal of near-infrared fluorescence at 700 nm. The detection limit of CDCI-HClO for HOCl is about 4 nM. Moreover, with the merit of a large Stokes shift (190 nm), CDCI-HClO was successfully applied to the imaging of HOCl in live cell, zebrafish, and living mice. All results demonstrated that CDCI-HClO is a valuable new NIR fluorescence imaging tool to detect hypochlorous acid in living systems.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, PR China
摘要：
Lysosomes are important subcellular organelles with acidic pH. The change of lysosomal pH can affect the normal function and activity of cells. To conveniently detect and visualize lysosomal pH changes, we designed herein a novel fluorescent probe NIR-Rh-LysopH. The probe is based on a Rhodamine 101 derivative, which was modified to include a fused tetrahydroquinoxaline ring to obtain near-infrared fluorescence and a methylcarbitol moiety to locate the lysosome. Based on the proton-induced spirolactam ring-opening mechanism, NIR-Rh-LysopH showed rapid, selective, sensitive, and reversible near-infrared fluorescence responses around 686 nm (Stokes shift 88 nm) with a pKa value of 5.70. From pH 7.4 to 4.0, about 285 folds of fluorescence enhancement was observed. Cell experiments showed that NIR-Rh-LysopH has low cytotoxicity and excellent lysosome-targeting ability. Moreover, NIR-Rh-LysopH was applied successfully to track lysosomal pH changes induced by drugs (such as chloroquine and dexamethasone), heatstroke, and redox stress. Thus, NIR-Rh-LysopH is very promising for conveniently tracking lysosomal pH changes and studying the related life processes.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Chem Biol Ctr, Key Lab Pesticide & Chem Biol,Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
摘要：
We report herein for the first time that the aggregation-induced emission and strong solid fluorescence of fluorescein derivatives can be realized by slightly modifying their structure, which provides a new option for AIEgens and solid fluorescent materials.
摘要：
A near-infrared (NIR) ratiometric fluorescent probe, NIR-Ratio-CO, was developed for rapid detection of carbon monoxide (CO) in both solution and living cells through the strategy of Pd(0)-mediated Tsuji-Trost reaction. This probe shows a rapid, highly specific and sensitive detection process for CO, accompanied by colorimetric and distinct ratiometric fluorescence changes at 655 and 592nm with a large Stokes shift up to 195nm. The detection limit for CO was measured to be about 61nM by the fluorescence method. In addition, this probe was successfully applied for ratiometric imaging of both exogenous and endogenous CO in living cells, indicating that it can be used as a novel tool for ratiometric fluorescent detection of CO in living systems.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
NIR fluorescent probe;CO;Rhodol;Large Stokes shift;Living systems
摘要：
Because of the biological importance of CO, the development of effective probes for the detection of CO in living systems is of great significance. In particular, near-infrared (NIR) fluorescence probes with good water solubility and large Stokes shift are indispensable tools for CO detection in vivo. However, such tools are extremely scarce. Herein, a modified rhodol dye was used to develop a novel NIR fluorescent probe (Rh-NIR-CO) for selective and sensitive detection of CO using the Pd-0-mediated Tsuji-Trost reaction. This probe shows good water solubility and rapid CO detecting ability in aqueous buffer at pH 7.4, accompanied by distinct colorimetric and turn-on NIR emission changes at 676 nm with a large Stokes shift (135 nm) and low detection limit (37 nM). Moreover, NIR fluorescence imaging of CO in living cells, zebrafish, and living mice was successfully applied with this probe. These excellent results highlighted Rh-NIR-CO as a promising new tool for in vitro and in vivo detection of CO.
期刊：
Sensors and Actuators B-Chemical,2020年309:127796 ISSN：0925-4005
通讯作者：
Feng, Guoqiang
作者机构：
[Feng, Guoqiang; Gong, Shengyi; Xia, Qingfeng; Feng, Shumin; Zheng, Junrong] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Int Joint Res Ctr Intelligent Biosensing Technol, Minist Educ,Coll Chem, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Int Joint Res Ctr Intelligent Biosensing Technol, Minist Educ,Coll Chem, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
Ferrous irons;Near-infrared fluorescent probe;N-oxide chemistry;Live cells;Lipid droplets;In vivo
摘要：
Ferrous ions (Fe2+) play an irreplaceable role in living animals. However, the lack of effective Fe2+ probes has seriously hindered the further study of Fe2+ in vivo. An easily obtained reaction-based fluorescent probe DCI-Fe (II) for real-time detection and imaging of Fe2+ both in cells and in vivo was reported in this work. DCI-Fe(II) shows distinct changes in color and highly selective turn-on near-infrared (NIR) fluorescence (similar to 120-fold enhancement at 700 nm) for Fe2+ with a rapid response (5 min), high sensitivity (51 nM of detection limit), and a marked Stokes shift (195 nm). Moreover, DCI-Fe(II) proves to be readily applicable to visualize Fe2+ in lipid droplets of living cells. Further imaging of Fe2+ in vivo with DCI-Fe(II) was also successful, indicating that DCI-Fe(II) holds great potential to promote the research of Fe2+ in life systems.
作者机构：
[Feng, Guoqiang; Deng, Yingzhen] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
摘要：
Drug-induced liver injury (DILI) is considered gradually as a serious public health issue, and hepatotoxicity has been regarded as the main clinical problem caused by it. We suspected that both the intracellular viscosity and peroxynitrite (ONOO(-)) levels in drug-induced hepatotoxicity tissue are higher than those in a healthy liver. For this reason, we have presented a fluorescent probe VO for multichannel imaging viscosity and ONOO(-) simultaneously. Experimental results showed that VO has satisfactory detection performance for both viscosity and ONOO(-), and based on the advantages of its lower cytotoxicity and pH-stabilities, VO was successfully employed to image viscosity and ONOO(-) in living cells and animals. More importantly, we use the probe to successfully showcase drug-induced hepatotoxicity by imaging viscosity and ONOO(-) induced by acetaminophen (APAP). All the results indicate that VO has great potential for the detection of viscosity and ONOO(-) and to assay drug-induced hepatotoxicity. Overall, this work offers a new detection tool/method for a deeper understanding of drug-induced organism injury.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Chem Biol Ctr, Key Lab Pesticide & Chem Biol,Minist Educ, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
关键词：
CO;Near-infrared;Fluorescent probe;Bioimaging;In vitro and in vivo
摘要：
A new near-infrared (NIR) fluorescent probe was developed for the detection of CO in living systems. This probe is based on a unique cyanine fluorophore and uses an allyl ether moiety as the reaction site. Optical studies in solution show that this probe has good water solubility and possesses desirable sensing properties for CO including rapid response, high selectivity and sensitivity, large Stokes shift (123 nm), distinct colorimetric changes, and significant turn-on NIR fluorescence changes at 715 nm with high signal-to-noise ratio. Ratiometric fluorescent detection of CO with this probe at 715 and 570 nm is also applicable. The sensing mechanism was proven to be a process of recovery of the fluorophore by removing the allyl group via Tsuji-Trost reaction. Furthermore, this probe was applied for detecting CO in living cells and animals with good bioimaging performance. Moreover, indicated by this probe, more CO could be produced under oxidative stress conditions. All the results indicate that this probe can be applied as a promising new tool for in vitro and in vivo detection of CO.
通讯机构：
[Feng, Guoqiang] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
关键词：
Bioimaging;Carbon monoxide;Large Stokes shift;Living cells and animals;NIR fluorescent probe
摘要：
Near-infrared (NIR) fluorescent probes with a large Stokes shift are very practical tools for bioimaging applications. Carbon monoxide (CO) is a key gaseous signal molecule and its imaging in living systems has attracted great attention in recent years. In this work, a very easy-to-get NIR fluorescent probe with a remarkable large pseudo-Stokes shift (238nm) for detection of CO was reported. This probe was found to show a rapid NIR fluorescent turn-on response for CO with high selectivity, high sensitivity and a low detection limit (38nM). Moreover, imaging CO in living cells and animals with this probe was successfully applied with a high signal-to-noise ratio. The results indicate that this probe can be used as a new practical tool for imaging of endogenous CO in living systems.
