作者机构：
[Wen H.; Gan T.; Shen B.; Zhong J.; Yang X.; Fu C.; Li R.] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, 430079, China;[Zhu Z.] Institute of Nanotechnology, College of Physical Science and Technology, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Rui Li; Xu Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China
关键词：
nanosized and microsized tellurium powder;HeLa cells;oxidative damage;reactive oxygen species (ROS);glutathione (GSH);DNA and protein crosslink (DPC);8-hydroxy-2’-deoxyguanosine (8-OHdG)
作者机构：
Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, China;Laboratory of Environmental Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan, China
摘要：
With the rapid development of nanotechnology and widespread use of nanoproducts, concerns have arisen regarding the ecotoxicity of these materials. In this paper, the photosynthetic toxicity and oxidative damage induced by nano Fe3O4 on a model organism, Chlorella vulgaris (C. vulgaris) in aquatic environment, were studied. The results showed that Nano-Fe3O4 was toxic to C. vulgaris and affected its content of chlorophyll a, malonaldehyde and glutathione, CO2 absorption, net photosynthetic rate, superoxide dismutase activity and inhibition of hydroxyl radical generation. At higher concentrations, compared with the control group, the toxicity of nano-Fe3O4 was significantly different. It suggested that nano-Fe3O4 is ecotoxic to C. vulgaris in aquatic environment.
作者机构：
College of Life Sciences, Central China Normal University, Wuhan 430079;Institute for Environmental Health, Chinese Center for Disease Control and Prevention
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
摘要：
In order to improve the efficiency of comet assay, which can be used in epidemiological research to detect genotoxicity of air pollutants, we undertook this study. Formaldehyde at 50μM was employed as contaminating agent to poison the human buccal cell before comet assay. During the process of comet assay, each pair of contaminated samples was treated with proteinase K of three different concentrations at 0.1mg/ml, 1mg/ml and 10mg/ml, respectively while other steps are kept the same. The result turned out to be that the 1 mg/ml proteinase K could lead to an ideal incubating effect. It was also proved that a 45-minute lysis had almost the same effect as the 120-min one. A further try was the reduction of the enzyme-incubation period to 45 min; the result showed that with the treatment of 1mg/ml proteinase K, a 45-minute lysis and a 45-minute enzyme-incubation as well can achieve a relatively ideal result.
摘要：
In order to improve the efficiency of comet assay, which can be used in epidemiological research to detect genotoxicity of air pollutants, we undertook this study. Formaldehyde at 50 mu M was employed as contaminating agent to poison the human buccal cell before comet assay. During the process of comet assay, each pair of contaminated samples was treated with proteinase K of three different concentrations at 0.1mg/ml, 1mg/ml and 10mg/ml, respectively while other steps are kept the same. The result turned out to be that the 1mg/ml proteinase K could lead to an ideal incubating effect. It was also proved that a 45-minute lysis had almost the same effect as the 120-min one. A further try was the reduction of the enzyme-incubation period to 45 min; the result showed that with the treatment of 1mg/ml proteinase K, a 45-minute lysis and a 45-minute enzyme-incubation as well can achieve a relatively ideal result.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年15:3556-3559 ISSN：0908-5920
通讯作者：
Li, R
作者机构：
[Chen, WG; Wei, F; Peng, JS; Wu, L; Hu, JC; Zhou, X; Q Z; Yao, DS; Liu, MQ; Yang, X; Li, R] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, R] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
会议时间：
SEP 04-09, 2005
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
formaldehyde;genotoxicity;comet assay;buccal cell;epidemiological study
摘要：
In this study, 60 families in the same community were investigated. Amnong these families, 30 were decorated within one year while the corresponding ones decorated at least 4 years ago. Buccal cells donated by people available in these families were employed to carry out single cell gel electrophoresis (SCGE, Comet assay, for genotoxicity testing). Analysis of the comet images showed that the presenting rate of "comet" as well as the tail moments of the buccal cells' DNA from people living in apartments decorated within a year were obviously higher (P < 0.01) than in those from people who lived in the apartments decorated several years ago, which lends strong support to our conclusion that people living in newly-decorated apartments are much more liable to be harmed by the formaldehyde released from their indoor decoration. In addition, high-quality decoration material tends to be less harmful to people.
作者机构：
College of Life Sciences, Central China Normal University, Wuhan 430079;Institute for Environmental Health, Chinese Center for Disease Control and Prevention
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
摘要：
In order to improve the efficiency of comet assay, which can be used in epidemiological research to detect genotoxicity of air pollutants, we undertook this study. Formaldehyde at 50μM was employed as contaminating agent to poison the human buccal cell before comet assay. During the process of comet assay, each pair of contaminated samples was treated with proteinase K of three different concentrations at 0.1mg/ml, 1mg/ml and 10mg/ml, respectively while other steps are kept the same. The result turned out to be that the 1 mg/ml proteinase K could lead to an ideal incubating effect. It was also proved that a 45-minute lysis had almost the same effect as the 120-min one. A further try was the reduction of the enzyme-incubation period to 45 min; the result showed that with the treatment of 1mg/ml proteinase K, a 45-minute lysis and a 45-minute enzyme-incubation as well can achieve a relatively ideal result.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年:2002-2006
通讯作者：
Yan, Y
作者机构：
[Liu, J; Lu, ZS; Yan, Y; Yang, X; Li, R; Wang, GX] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yan, Y] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
Formaldehyde;Wood-based board;Emission model
摘要：
In order to understand the rule of formaldehyde emission from the wood-based boards bought from the construction material markets of China, a formaldehyde emission study was undertaken in our laboratory during July 2001-March 2002. The chamber experiments were carried out with the international standard method and the chamber parameters were controlled to T=23.0 degrees C +/- 0.5 degrees C, RH=45%+/- 5%, ACH=1.0 +/- 0.03 h(-1), Loading factor=1.0 +/- 0.03 m(2)m(-3). By the analysis of formaldehyde emitted from 11 types of wood-based boards taken from construction material markets in Wuhan City of China, the experimental results show that the emission concentrations in the chamber obviously varied along with time. We had tried to use the mathematic exponent model y = y(0) + A(1)e(-(x-x0)/t1), and found which fit the short-term and long-term emission procession with chamber concentrations vs escape time very well.
摘要：
Purpose: Whether formaldehyde induces DNA breakage or not is still in debate. So far it is not clear whether dose-effect between formaldehyde and DNA breakage takes place while the DPC exists. Approach: In this study we had chosen human buccal cells as materials to explore the genotoxicity of liquid and gaseous formaldehyde with comet assay. Findings: The results showed that liquid formaldehyde induced DNA breakage in the range from 5 mu M to 10 mu M (P<0.05, compared with control group), and 7.5 mu M was found to induce DNA breakage most. The DNA crosslinks were significantly found at 15 mu M, 30 mu M and 50 mu M (P<0.01, compared with 7.5 mu M group). DNA breakage caused by gaseous formaldehyde was found at 0.5 mg/m(3) and 1.0 mg/m(3) (P<0.01, compared with control group) but DNA crosslinks; at 3.0 mg/m(3) Significantly. Conclusions: The results suggest that formaldehyde induces DNA breakage in a very low concentration range but induces DNA crosslinks in a higher range.
