纳米Al_2 O_3经气道滴注致小鼠脏器损伤和炎症反应的研究
作者：
崔海燕;李丹;王吉燕;路曼曼;黄佳伟;...
期刊：
环境科学学报 ,2019年39(3):969-977 ISSN：0253-2468
通讯作者：
Li, R.
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉430079;[黄佳伟; 路曼曼; 李睿; 李丹; 赵云; 王吉燕; 崔海燕] 华中师范大学
通讯机构：
Key Laboratory of Genetic Regulation and Integrated Biology of Hubei Province, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
纳米氧化铝（nAl2O3）;脏器;氧化应激;炎症反应;维生素E
摘要：
为探讨纳米氧化铝(nAl_2O_3)气道滴注对小鼠脏器的毒性作用,本研究将Balb /c小鼠随机分成6组:生理盐水组、50 mg·kg~(-1)·d~(-1) Vit E(维生素E)组、0.5 mg·kg~(-1)·d~(-1) nAl_2O_3组、5 mg·kg~(-1)·d~(-1) nAl_2O_3组、50 mg·kg~(-1)·d~(-1) nAl_2O_3组、nAl_2O_3 50+Vit E组(50 mg·kg~(-1)·d~(-1) nAl_2O_3 +50 mg·kg~(-1)·d~(-1)Vit E) .实验周期为21 d,气道滴注暴露,隔天滴注,维生素E灌胃阻断.染毒结束后,检测肺部、脾脏、肝脏和肾脏中活性氧(Reactive Oxide Species,ROS)和还原型谷胱甘肽(Glutathione,GSH)含量,并进行肺部病理学观察和肺泡灌洗液细胞计数.结果表明:与对照组相比, nAl_2O_3剂量为0.5 mg·kg~(-1)·d~(-1)时,小鼠肺部ROS含量增加(p<0.05) ,肝脏GSH含量下降(p<0.05) ; nAl_2O_3剂量为5和50 mg·kg~(-1)·d~(-1)时,小鼠肺部、脾脏、肝脏和肾脏ROS含量均显著增加(p<0.05) ,肺部和肝脏GSH含量均显著下降(p<0.05) ;且小鼠肺部出现支气管壁增厚、气道腔皱缩、组织纤维化等气道重塑和嗜酸性粒细胞等炎症细胞浸润现象.而抗氧化剂维生素E的阻断显著降低了肝脏ROS含量,有效恢复了肺部GSH活性(p<0.01) ,且缓解了肺部气道重塑和炎症细胞浸润现象(p<0.05) .研究表明,nAl_2O_3经气道滴注染毒后,不仅会对小鼠肺部造成损伤和炎症反应,同时也能够对脾脏、肝脏和肾脏造成氧化损伤.本研究可为纳米材料的安全性应用及其潜在危害的预防提供科学依据.
语种：
中文
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Vasodilatory effect of formaldehyde via the NO/cGMP pathway and the regulation of expression of KATP, BKCa and L-type Ca(2+) channels.
作者：
Zhao, Yun;Ge, Jing;Li, Xiaoxiao;Guo, Qing;Zhu, Yuqing;...
期刊：
Toxicology Letters ,2019年312:55-64 ISSN：0378-4274
通讯作者：
Yang, Xu;Li, Rui
作者机构：
[Guo, Qing; Yang, X; Li, R; Li, Rui; Li, Xiaoxiao; Yang, Xu; Zhao, Yun; Song, Jing; Ge, Jing; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Luoping] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.;[Guo, Qing] Huazhong Univ Sci & Technol, Sch Publ Hlth, Hangkong Rd, Wuhan 430030, Hubei, Peoples R China.;[Zhu, Yuqing] Zhejiang Univ, Sch Med, Ctr Stem Cell & Regenerat Med, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China.
通讯机构：
[Yang, X; Li, R] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
关键词：
Formaldehyde;Ion channels;NO/cGMP pathway;Rat aortas;Vasorelaxation
摘要：
Formaldehyde (FA), a well-known toxic gas molecule similar to nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S), is widely produced endogenously via numerous biochemical pathways, and has a number of physiological roles in the biosystem. We attempted to investigate the vasorelaxant effects of FA and their underlying mechanisms. We found that FA induced vasorelaxant effects on rat aortic rings in a concentration-dependent manner. The NO/cyclic guanosine 5' monophosphate (cGMP) pathway was up-regulated when the rat aortas were treated with FA. The expression of large-conductance Ca(2+)-activated K(+) (BKCa) channel subunits alpha and beta of the rat aortas was increased by FA. Similarly, the levels of ATP-sensitive K(+) (KATP) channel subunits Kir6.1 and Kir6.2 were also up-regulated when the rat aortas were incubated with FA. In contrast, levels of the L-type Ca(2+) channel (LTCC) subunits, Cav1.2 and Cav1.3, decreased dramatically with increasing concentrations of FA. We demonstrated that the regulation of FA on vascular contractility may be via the up-regulation of the NO/cGMP pathway and the modulation of ion channels, including the upregulated expression of the KATP and BKCa channels and the inhibited expression of LTCCs. Further study is needed to explore the in-depth mechanisms of FA induced vasorelaxation.
语种：
英文
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Dermal exposure to nano-TiO2 induced cardiovascular toxicity through oxidative stress, inflammation and apoptosis
作者：
Zhang, Qian;Liu, Zhimin;Du, Junting;Qin, Wei;Lu, Manman;...
期刊：
JOURNAL OF TOXICOLOGICAL SCIENCES ,2019年44(1):35-45 ISSN：0388-1350
通讯作者：
Yuan, Junlin
作者机构：
[Lu, Manman; Yuan, Junlin; Liu, Zhimin; Li, Rui; Li, Xiaoxiao; Zhang, Qian; Cui, Haiyan; Du, Junting; Qin, Wei; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Yuan, Junlin] C;Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Nano-titanium dioxide (nano-TiO2);Cardiovascular toxicity;Oxidative stress;Inflammation;Cytotoxicity;Apoptosis
摘要：
Due to its excellent properties such as ultraviolet obscuration, chemical stability and small particle size, nano-titanium dioxide (nano-TiO 2 ) is widely used, particularly in sunblock products. The skin is therefore a chief route for exposure. Studies have found that oral or respiratory exposure to nano-TiO 2 has an adverse impact on the cardiovascular system. The relationship between dermal exposure to nano-TiO 2 and cardiovascular system toxicity, particularly the causative mechanisms, remain unclear. In this study, we used Balb/c mice to evaluate cardiovascular toxicity from nano-TiO 2 dermal exposure, and the underlying mechanisms associated with the human umbilical vein endothelial cells (HUVECs) were explored. Our results showed that nano-TiO 2 treatment resulted in an obvious increase in reactive oxygen species and 8-hydroxy-2’-deoxyguanosine, indicating the appearance of oxidative stress. Moreover, the levels of inflammatory biomarkers, such as immunoglobulin E, soluble intercellular adhesion molecule-1, interleukin-8, and hypersensitive C-reactive protein, also increased. Exposing HUVECs to nano-TiO 2 led to a decline in cell vitality, and an increase in caspase-3 levels, suggesting that nano-TiO 2 exposure caused cytotoxicity and even cell apoptosis. Interestingly, neutralizing oxidative stress by administering Vitamin E was shown to reduce the inflammatory response and cytotoxicity. Our findings suggest that nano-TiO 2 can injure the cardiovascular system via dermal exposure, and does this via oxidative stress-induced inflammation and cytotoxicity. Vitamin E treatment may be a strategy to mitigate the damage. © 2019, Japanese Society of Toxicology. All rights reserved.
语种：
英文
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New insight into Alzheimer's disease: Light reverses Aβ-obstructed interstitial fluid flow and ameliorates memory decline in APP/PS1 mice
作者：
Xiangpei Yue;Yufei Mei;Yun Zhang;Zheng Tong;Dehua Cui;...
期刊：
Alzheimer's & Dementia: Translational Research & Clinical Interventions ,2019年5(1):671-684 ISSN：2352-8737
通讯作者：
Tong, Z.
作者机构：
[Chunli Duan; Ge Gao; Hui Yang] Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China;[Dehua Cui; Jun Yang; Aibo Wang; Rui Wang] Department of Radiology, Peking University Third Hospital, Key Laboratory of Magnetic Resonance Imaging Equipment and Technique, Beijing, China;Corresponding author. Tel: +86-010-83950362;Fax: +86-010-83950363.;∗Corresponding author. Tel: 604-822-8019
通讯机构：
[Weihong Song; Hongbin Han] D;[Zhiqian Tong] L;Department of Radiology, Peking University Third Hospital, Key Laboratory of Magnetic Resonance Imaging Equipment and Technique, Beijing, China<&wdkj&>Corresponding author. Tel: +86-010-83950362;Fax: +86-010-83950363.<&wdkj&>∗Corresponding author. Tel: 604-822-8019;Fax: 604-822-7981.<&wdkj&>∗∗Corresponding author. Tel: +86-010-82266972
关键词：
Red light (RL);Alzheimer's disease (AD);Amyloid β (Aβ);Formaldehyde (FA);Formaldehyde dehydrogenase (FDH);Extracellular space (ECS);Interstitial fluid (ISF)
摘要：
Introduction: Pharmacological therapies to treat Alzheimer's disease (AD) targeting "Abeta" have failed for over 100 years. Low levels of laser light can disassemble Abeta. In this study, we investigated the mechanisms that Abeta-blocked extracellular space (ECS) induces memory disorders in APP/PS1 transgenic mice and addressed whether red light (RL) at 630 nm rescues cognitive decline by reducing Abeta-disturbed flow of interstitial fluid (ISF). Methods: We compared the heating effects on the brains of rats illuminated with laser light at 630, 680, and 810 nm for 40 minutes, respectively. Then, a light-emitting diode with red light at 630 nm (LED-RL) was selected to illuminate AD mice. The changes in the structure of ECS in the cortex were examined by fluorescent double labeling. The volumes of ECS and flow speed of ISF were quantified by magnetic resonance imaging. Spatial memory behaviors in mice were evaluated by the Morris water maze. Then, the brains were sampled for biochemical analysis. Results: RL at 630 nm had the least heating effects than other wavelengths associated with ~49% penetration ratio into the brains. For the molecular mechanisms, Abeta could induce formaldehyde (FA) accumulation by inactivating FA dehydrogenase. Unexpectedly, in turn, FA accelerated Abeta deposition in the ECS. However, LED-RL treatment not only directly destroyed Abeta assembly in vitro and in vivo but also activated FA dehydrogenase to degrade FA and attenuated FA-facilitated Abeta aggregation. Subsequently, LED-RL markedly smashed Abeta deposition in the ECS, recovered the flow of ISF, and rescued cognitive functions in AD mice. Discussion: Abeta-obstructed ISF flow is the direct reason for the failure of the developed medicine delivery from superficial into the deep brain in the treatment of AD. The phototherapy of LED-RL improves memory by reducing Abeta-blocked ECS and suggests that it is a promising noninvasive approach to treat AD.
语种：
英文
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PM2.5经皮肤暴露和气道滴注对小鼠肝脏的不同影响
作者：
路曼曼;宋静;鲁娴娴;张倩;崔海燕;...
期刊：
化学与生物工程 ,2018年35(12):14-19 ISSN：1672-5425
作者机构：
华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,湖北武汉,430079;[路曼曼; 宋静; 李睿; 鲁娴娴; 张倩; 崔海燕] 华中师范大学
关键词：
皮肤暴露;气道滴注;肝脏;氧化应激
摘要：
大气细颗粒物( PM2.5 )可通过呼吸、皮肤接触等途径进入机体,对机体健康产生危害.为了探究 PM2.5 经不同暴露途径对机体产生的不同影响,将小鼠随机分为:对照组,20 μ g·kg^-1 ·d^-1 、100 μ g·kg^-1 ·d^-1 、500 μ g·kg^-1·d^-1 PM2.5 皮肤暴露组和 20 μg·kg^-1 ·d^-1 、100 μ g·kg^-1 ·d^-1 、500 μ g·kg^-1 ·d^-1 PM2.5 气道滴注组.结果表明,随着 PM2.5 浓度的增加,小鼠肝脏细胞空泡化程度增强,同时小鼠肝脏 ROS 、 MDA 、 8 - OHdG 和 ALT 含量升高;PM2.5 经皮肤暴露和气道滴注均可对小鼠肝脏产生不利影响,且随着 PM2.5 浓度的增加损伤越严重,这可能与其诱导的氧化应激有关,且经气道滴注产生的损伤更严重.
语种：
中文
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Exposure to formaldehyde perturbs the mouse gut microbiome
作者：
Guo, Junhui;Zhao, Yun;Jiang, Xingpeng
（ ;Li, Rui;Xie, Hao;...
期刊：
Genes ,2018年9(4) ISSN：2073-4425
通讯作者：
Yang, Xu;Zhang, Luoping
作者机构：
[Xie, Bo; Ge, Leixin; Li, Rui; Yang, Xu; Zhao, Yun; Guo, Junhui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Luoping; Guo, Junhui] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.;[Xie, Hao; Guo, Junhui] Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Hubei, Peoples R China.;[Jiang, Xingpeng] Cent China Normal Univ, Sch Comp, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Yang, Xu] C;[Zhang, Luoping] U;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.
关键词：
16s rRNA gene;Formaldehyde (FA);Gut microbiome;High-throughput;Murine model
摘要：
Exposure to Formaldehyde (FA) results in many pathophysiological symptoms, however the underlying mechanisms are not well understood. Given the complicated modulatory role of intestinal microbiota on human health, we hypothesized that interactions between FA and the gut microbiome may account for FA’s toxicity. Balb/c mice were allocated randomly to three groups: a control group, a methanol group (0.1 and 0.3 ng/mL MeOH subgroups), and an FA group (1 and 3 ng/mL FA subgroups). Groups of either three or six mice were used for the control or experiment. We applied high-throughput sequencing of 16S ribosomal RNA (rRNA) gene approaches and investigated possible alterations in the composition of mouse gut microbiota induced by FA. Changes in bacterial genera induced by FA exposure were identified. By analyzing KEGG metabolic pathways predicted by PICRUSt software, we also explored the potential metabolic changes, such as alpha-Linolenic acid metabolism and pathways in cancer, associated with FA exposure in mice. To the best of our knowledge, this preliminary study is the first to identify changes in the mouse gut microbiome after FA exposure, and to analyze the relevant potential metabolisms. The limitation of this study: this study is relatively small and needs to be further confirmed through a larger study. © 2018 by the authors.
语种：
英文
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邻苯二甲酸二异癸酯对小鼠学习记忆的影响
作者：
路雨;李瑶;胡赢丹;李秋林;赵云;...
期刊：
中国环境科学 ,2018年38(1):361-368 ISSN：1000-6923
通讯作者：
Li, Rui(ruli@mail.ccnu.edu.cn)
作者机构：
[路雨; 李瑶; 胡赢丹; 李秋林; 赵云; 邹鉴; 刘潇童; 杨旭; 李睿] 华中师范大学生命科学学院, 遗传调控与整合生物学湖北省重点实验室, 湖北, 武汉, 430079
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
邻苯二甲酸二异癸酯;学习记忆;氧化损伤;白藜芦醇;Morris水迷宫
摘要：
为研究增塑剂邻苯二甲酸二异癸酯(DIDP)对小鼠学习和记忆能力的影响,以白藜芦醇(Res)作为保护剂,将42只雄性昆明小鼠随机分为6组,每组7只,分别为:生理盐水组、1.5,15,150 mg/(kg·d) DIDP组、20 mg/(kg·d) Res组、150 mg/(kg·d) DIDP+20 mg/(kg·d) Res组.连续灌胃染毒9d,期间同时进行Morris水迷宫实验.第10 d将小鼠处死,取出脑组织,检测活性氧簇(ROS)、谷胱甘肽(GSH)、丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)、肿瘤坏死因子(TNF-α)、白细胞介素1β(IL-1β)的含量.并对小鼠海马体切片进行H&E染色,观察病理变化.结果显示,15,150 mg/(kg·d) DIDP染毒可导致小鼠的学习和记忆能力显著低于对照组,同时可诱发脑组织产生氧化应激,并促进炎症因子释放,而Res能有效减弱脑组织氧化应激水平.由上可得,DIDP致小鼠学习和记忆能力下降可能与其引起的脑组织中海马体的氧化损伤有关,同时Res可能通过降低DIDP引起的氧化应激进而减轻其对小鼠造成的学习记忆损伤.
语种：
中文
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PM_(2.5)和甲醛联合暴露致小鼠肺损伤及其分子机制的研究
作者：
閤静;赵云;黄佳伟;张萍;潘雯;...
期刊：
生态毒理学报 ,2018年13(3):87-93 ISSN：1673-5897
作者机构：
[閤静; 赵云; 黄佳伟; 张萍; 潘雯; 尤安琪; 黄希; 杨旭; 李睿] 华中师范大学生命科学学院, 武汉, 430079
关键词：
甲醛;复合暴露;肺组织损伤;分子机制
摘要：
我国城市当前普遍存在室外大气PM_(2.5)与室内甲醛(FA)联合污染状况,二者均被报道在单独暴露下可以导致肺损伤并诱导和诱发哮喘的急性发作,但其联合污染的具体效应,以及分子机制目前尚不清楚。为探究PM_(2.5)和/或甲醛暴露对小鼠的肺损伤及其可能的机制,分别将雄性Balb/c小鼠分为以下6组:对照组,AZD8055组,PM_(2.5)组,FA组,PM_(2.5) +FA组,PM_(2.5) +FA+ AZD8055组。染毒结束后,观察肺组织病理学变化;检测肺组织氧化损伤,活性氧(reactive oxygen species, ROS),还原型谷胱甘肽(glutathione, GSH)和丙二醛(malondialdehyde, MDA)的含量,DNA损伤,DNA-蛋白质交联( DNA-protein crosslink,DPC)系数和8羟基脱氧鸟苷(8-OH-dG)的含量,以及细胞凋亡、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的含量。结果表明,当吸入气态甲醛浓度为3 mg·m~(-3),气道滴注PM_(2.5)浓度为2.5 mg·mL~(-1)时,肺组织出现不同程度的支气管重塑和炎症细胞浸润。ROS显著上升,GSH显著下降,DPC、8-OH-dG以及Caspase-3都显著上升。添加AZD8055后,肺组织损伤效应更加显著。PM_(2.5)复合甲醛的暴露导致小鼠肺损伤具有协同作用,氧化应激及其下游的DNA损伤可能是甲醛联合PM_(2.5)致小鼠肺损伤的一种重要机制。
语种：
中文
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DIDP通过线粒体- Caspase途径诱导昆明小鼠肝脏损伤的研究
作者：
李瑶;路雨;胡赢丹;李秋林;赵云;...
期刊：
环境科学研究 ,2018年31(11):1957-1964 ISSN：1001-6929
通讯作者：
Li, R.
作者机构：
[李瑶; 路雨; 胡赢丹; 李秋林; 赵云; 李睿] 华中师范大学, 遗传调控与整合生物学湖北省重点实验室, 湖北, 武汉, 430079
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
邻苯二甲酸二异癸酯;氧化应激;线粒体损伤;细胞凋亡;肝脏
摘要：
为探究DIDP（Di-iso-decyl phthalate,邻苯二甲酸二异癸酯）对肝脏的影响及其可能的分子机制,以昆明小鼠为研究对象,选用Res（resveratrol,白藜芦醇）为抗氧化剂,分别设置对照组,0. 15、1. 5、15、150 mg/（kg·d）DIDP组,Res组,150 mg/（kg·d）DIDP+Res组,灌胃染毒9 d后,对小鼠肝脏切片进行HE染色观察,并检测ROS（reactive oxygen,活性氧）、GSH（glutathione,谷胱甘肽）、MDA（malondialdehyde,丙二醛）、Cyt-C（cytochromec,细胞色素C）、Caspase-3和血清中的ALT（alanine aminotransferase,丙氨酸氨基转移酶）含量.结果表明:与对照组相比,15、150 mg/（kg·d）DIDP组小鼠血清中ALT含量极显著上升（P<0. 01）;HE染色结果显示,15、150 mg/（kg·d）DIDP组小鼠出现肝细胞水肿、肝索紊乱、肝窦以及肝中央静脉扩张等现象;15、150 mg/（kg·d）DIDP组小鼠肝脏ROS含量显著上升（P<0. 05）,GSH含量显著下降（P<0. 05）,150 mg/（kg·d）DIDP组小鼠肝脏MDA含量极显著上升（P<0. 01）;1. 5、15、150 mg/（kg·d） DIDP组小鼠肝脏中c （Cyt-C）极显著上升（P <0. 01）;15、150 mg/（kg·d） DIDP组小鼠肝脏中Caspase-3表达量极显著上升（P<0. 01）. DIDP染毒剂量的增加对肝脏的各种损伤程度呈上升趋势,Res可减轻上述DIDP对肝脏造成的各种损伤.研究显示,15、150 mg/（kg·d）DIDP可诱导肝脏组织氧化应激水平上升,进而造成线粒体损伤,导致细胞凋亡,造成肝功能受损,因此,线粒体-Caspase途径可能是DIDP诱导肝脏损伤的潜在机制之一.
语种：
中文
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Differential Health Effects of Constant versus Intermittent Exposure to Formaldehyde in Mice: Implications for Building Ventilation Strategies
作者：
Zhang, Xu;Zhao, Yun;Song, Jing;Yang, Xu;Zhang, Junfeng;...
期刊：
Environmental Science & Technology ,2018年52(3):1551-1560 ISSN：0013-936X
通讯作者：
Zhang, Yinping;Li, Rui
作者机构：
[Zhang, Xu; Zhang, Yinping] Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.;[Zhang, Xu; Zhang, Yinping] Beijing Key Lab Indoor Air Qual Evaluat & Control, Beijing 100084, Peoples R China.;[Li, Rui; Yang, Xu; Zhao, Yun; Song, Jing] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Junfeng] Duke Univ, Global Hlth Inst, Durham, NC USA.;[Zhang, Junfeng] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA.
通讯机构：
[Zhang, Yinping] T;[Zhang, Yinping] B;[Li, Rui] C;Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.;Beijing Key Lab Indoor Air Qual Evaluat & Control, Beijing 100084, Peoples R China.
摘要：
Formaldehyde, an air pollutant in the indoor environment, may have severe effects on human health. The aim of this study is to compare the health effects caused by intermittent exposure to formaldehyde (based on real monitoring) to those caused by exposures at constant concentration. Health effects explored in this study including the oxidative stress, histopathological changes, inflammatory responses, etc. Mice were divided into three groups and exposed to intermittent concentration formaldehyde (0.8 ppm for 12 h and 0 ppm for another 12 h), or constant concentration formaldehyde (0.4 ppm for 24 h) or zero concentration formaldehyde (reference) per day for 7, 14, and 28 days. Following these exposures, bronchoalveolar lavage fluid (BALF), lung tissue and lung tissue homogenate were prepared to measure biomarkers of oxidative stress (ROS, MDA, GSH), histopathological changes, inflammatory responses (EOS, NEU, LYM, IL-4, IL-5, IL-13, IL-6, IL-17A, NF-κB, IL-1β) and apoptosis (caspase-3). Compared to the constant exposure, intermittent exposure to fluctuating formaldehyde concentrations resulted in more profound increases in numbers of inflammatory cells in the BALF, greater biological alterations including apoptosis. The findings imply that with the same average indoor formaldehyde concentrations over the same time, a ventilation strategy to avoid higher peak concentrations would lead to lower health risks. © 2018 American Chemical Society.
语种：
英文
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Oral exposure to diisodecyl phthalate aggravates allergic dermatitis by oxidative stress and enhancement of thymic stromal lymphopoietin
作者：
Shen, Shiping;Li, Jinquan;You, Huihui;Wu, Zhuo;Wu, Yang;...
期刊：
Food and Chemical Toxicology ,2017年99:60-69 ISSN：0278-6915
通讯作者：
Yang, Xu;Chen, Mingqing
作者机构：
[Guo, Qing; Shen, Shiping; Li, Jinquan; Chen, Mingqing; Li, Rui; You, Huihui; Li, Xiaoxiao; Yang, Xu; Zhao, Yun; Wu, Zhuo; Zhu, Yuqing; Yang, X; Chen, MQ] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Zhao, Yun] Hubei Univ Sci & Technol, Coll Basic Med Sci, Xianning 437100, Peoples R China.
通讯机构：
[Yang, X; Chen, MQ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Allergic contact dermatitis;Diisodecyl phthalate (DIDP);NF-kappa B;Oxidative stress;Thymic stromal lymphopoietin (TSLP)
摘要：
Diisodecyl phthalate (DIDP) is extensively used as an environmentally friendly plasticizer. However, little is known about the adverse effects and the underlying mechanisms of DIDP exposure on immunological diseases. We aimed to determine the role and mechanisms of DIDP exposure in allergic contact dermatitis-like skin lesions. We show that oral DIDP exposure can aggravate allergic dermatitis in mice. Moreover, an increase of ROS, total serum IgE and IL-4 levels were concomitant with this deterioration. We detected the expression of Thymic stromal lymphopoietin (TSLP) and the activation of STATs and NF-kappa B signal pathways. The data indicated that DIDP in combination with FITC triggers TSLP production. Our results also suggested that DIDP exacerbated the activation of NF-kappa B signal pathways, with an enhancement in TSLP expression, which potentiated the activation of STATs and the degranulation of mast cells in the skin, and finally exacerbated allergic dermatitis. The study also suggested that melatonin enhanced the expression of Nrf2, up-regulated the antioxidant genes HO-1 and NQO1, reduced the levels of oxidative stress and TSLP, and alleviated allergic dermatitis. The results demonstrated that DIDP exacerbated allergic dermatitis through oxidative stress and enhanced TSLP production. (C) 2016 Elsevier Ltd. All rights reserved.
语种：
英文
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信息化视角下本科生双语课程教学实践与思考——以《分子生物学》课程为例
作者：
李睿;邱杏;朱家华;甘露;崔鸿
期刊：
中国教育信息化 ,2017年(19):80-84 ISSN：1673-8454
作者机构：
华中师范大学生命科学学院,湖北武汉,430079;华中师范大学教师教育学院,湖北武汉,430079;[李睿; 邱杏; 朱家华; 甘露; 崔鸿] 华中师范大学
关键词：
信息化;分子生物学;双语教学
摘要：
信息化理念与《分子生物学（双语）》教学的融合是立足教育信息化背景、生命科学国际化视野下的有益尝试。本文论证了信息化理念与教学融合的理论基础与策略,依托华中师范大学信息化环境,结合课程特点展开教学实践,基于问卷调查、访谈,对教学实践进行了总结与反思,结果显示,信息化理念与教学融合对于提升教学实效、促进师生互动、提升学生高级思维能力和学习动力等方面有积极作用,但在信息化资源建设、信息技术在课堂中的有效介入等方面仍存在疑虑与困惑。
语种：
中文
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甲醛复合PM_(2.5)致小鼠血液毒性的研究
作者：
閤静;郭晴;江清英;卢美玲;赵云;...
期刊：
中国环境科学 ,2017年37(7):2740-2748 ISSN：1000-6923
通讯作者：
Li, Rui(ruili@mail.ccnu.edu.cn)
作者机构：
[卢美玲; 李睿; 赵云; 朱雨晴; Ge, Jing; 江清英; 杨旭; 郭晴] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
甲醛;复合暴露;血液毒性
摘要：
为探究甲醛(FA)复合PM_(2.5)对小鼠血液毒性的影响及其可能的机制,分别从血液,造血器官(骨髓、脾脏),髓系祖细胞3个层面的损伤进行系统研究.以雄性Balb/c小鼠为实验对象,把小鼠随机分为4组(对照组、PM_(2.5)组、FA组、PM_(2.5)+ FA组),对小鼠进行全血细胞计数;观察骨髓、脾脏病理学的变化;检测骨髓、脾脏及髓系祖细胞的氧化损伤(ROS,GSH),DNA损伤(DPC,8-OH-dG),细胞凋亡(caspase-3)指标.结果表明,骨髓、脾脏分别出现不同程度的病理学变化;骨髓、脾脏,髓系祖细胞的氧化损伤,DNA损伤,细胞凋亡水平也有上升趋势.FA复合PM_(2.5)暴露会导致小鼠的血液毒性,氧化应激,及其下游的DNA损伤可能是FA复合PM_(2.5)致小鼠血液毒性的一种重要机制.
语种：
中文
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PM2.5和甲醛复合暴露致小鼠造血毒性及其分子机制的研究
作者：
閤静;杨旭;李睿
作者机构：
[閤静; 杨旭; 李睿] 华中师范大学生命科学学院
会议名称：
2017环境与公共健康学术会议暨中国环境科学学会环境医学与健康分会、中国毒理学会生化与分子毒理专业委员会2017年年会
会议时间：
2017-11-10
会议地点：
中国广东广州
关键词：
甲醛;复合暴露;造血毒性;分子机制
摘要：
我国城市当前普遍存在大气PM2.5与室内甲醛复合污染状况,二者均被报道在单独暴露可引起造血毒性[1,2],但分子机制尚不清楚。为探究FA复合PM2.5对小鼠造血毒性的影响及其可能机制,分别从血液,造血器官（骨髓,脾脏）,髓系祖细胞三个层面进行系统研究。将雄性Balb/c小鼠分别暴露于PM2.5和/或甲醛中。结果表明,小鼠骨髓、脾脏出现不同程度病理学变化,减少了外周血血细胞的数量和
语种：
中文
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Graphene Oxide Thin Film with Dual Function Integrated into a Nanosandwich Device for in Vivo Monitoring of Interleukin-6
作者：
Qi, Meng;Huang, Jiawei;Wei, Hui;Cao, Chaomin;Feng, Shilun;...
期刊：
ACS Applied Materials & Interfaces ,2017年9(48):41659-41668 ISSN：1944-8244
通讯作者：
Liu, Guozhen
作者机构：
[Cao, Chaomin; Liu, Guozhen; Qi, Meng; Wei, Hui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Li, Rui; Huang, Jiawei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.;[Feng, Shilun; Goldys, Ewa M.; Liu, Guozhen] Macquarie Univ, ARC Ctr Excellence Nanoscale Biophoton CNBP, Sydney, NSW 2109, Australia.;[Liu, Guozhen] Macquarie Univ, Dept Mol Sci, Sydney, NSW 2109, Australia.;[Guo, Qing] Huazhong Univ Sci & Technol, Sch Publ Hlth, 13 Hangkong Rd, Wuhan 430030, Hubei, Peoples R China.
通讯机构：
[Liu, Guozhen] C;[Liu, Guozhen] M;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;Macquarie Univ, ARC Ctr Excellence Nanoscale Biophoton CNBP, Sydney, NSW 2109, Australia.;Macquarie Univ, Dept Mol Sci, Sydney, NSW 2109, Australia.
关键词：
graphene oxides;nanosandwich device;cytokines;in vivo detection;aryldiazonium salt chemistry
摘要：
Graphene oxide (GO), with its exceptional physical and chemical properties and biocompatibility, holds a tremendous potential for sensing applications. In this study, GO, acting both as the electron-transfer bridge and the signal reporter, was attached on the interface to develop a label-free electrochemical nanosandwich device for detection of interleukin-6 (IL-6). First, a single layer of GO was covalently modified on gold electrodes, followed by attachment of anti-IL-6 capture antibody to form the sensing interface. The 4-aminophenyl phosphorylcholine was further attached to the surface of GO to minimize nonspecific protein adsorption. For reporting the presence of analyte, the anti-IL-6 detection antibody was covalently modified to the GO, which has been integrated with the redox probe Nile blue (NB). Finally, a nanosandwich assay was fabricated on gold surfaces for detection of IL-6 on the basis of the electrochemical signal of NB. The prepared nanosandwiches demonstrated high selectivity and stability for detection of IL-6 over the range of 1-300 pg mL -1 with the lowest detectable concentration of 1 pg mL -1 . The device was successfully used for monitoring of IL-6 secretion in RAW cells and live mice. By tailoring the GO surface with functional components, such devices were able to detect the analyte in vivo without causing inflammatory response. © 2017 American Chemical Society.
语种：
英文
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PM2.5和甲醛复合暴露致小鼠骨髓、脾脏和髓系祖细胞损伤和维生素E的保护作用
作者：
閤静;杨旭;李睿
作者机构：
[閤静; 杨旭; 李睿] 华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室
会议名称：
2017中国环境科学学会科学与技术年会
会议时间：
2017-10-20
会议地点：
厦门
会议论文集名称：
2017中国环境科学学会科学与技术年会论文集
关键词：
甲醛;骨髓;脾脏;髓系祖细胞
摘要：
为探究甲醛（FA）复合PM2.5对小鼠造血毒性的影响及其可能的机制,分别从血液,造血器官（骨髓,脾脏）,髓系祖细胞三个层面进行系统研究。将雄性Balb/c小鼠分别单独暴露于PM2.5（20、200μg/kg.d）、甲醛（0.5、3mg/m3）、复合暴露于PM2.5+FA(20 PM2.5+0.5 FA、20 PM2.5+3FA、200 PM2.5+0.5 FA
语种：
中文
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气态甲醛波动形式暴露对小鼠健康危害研究
作者：
张旭;赵云;宋静;杨旭;张寅平;...
作者机构：
[张旭; 赵云; 宋静; 杨旭; 张寅平; 李睿] 清华大学建筑技术科学系;[张旭; 赵云; 宋静; 杨旭; 张寅平; 李睿] 室内空气品质控制与评价北京市重点实验室;[张旭; 赵云; 宋静; 杨旭; 张寅平; 李睿] 华中师范大学生命科学学院
会议名称：
2017环境与公共健康学术会议暨中国环境科学学会环境医学与健康分会、中国毒理学会生化与分子毒理专业委员会2017年年会
会议时间：
2017-11-10
会议地点：
中国广东广州
关键词：
甲醛;波动形式;健康效应
摘要：
甲醛作为一种常见的室内空气污染物,能够导致哮喘、肺癌、白血病等一系列疾病。人员活动、净化策略、通风方式以及散发源特性等均会对室内甲醛浓度造成影响,进而导致不同波动形式的甲醛产生。比较不同甲醛波动形式引起的健康危害有助于加深对于甲醛毒性的理解,进而对室内甲醛的控制策略进行指导,以得到最节能、节滤材的室内健康环境营造策略。本研究基于住户中实际测得的甲醛浓度波动形式,以Balb/c小鼠为实验对象,比较了
语种：
中文
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Oxidized graphene-aggravated allergic asthma is antagonized by antioxidant vitamin E in Balb/c mice
作者：
Shang, Shuai;Li, Jinquan;Zhao, Yun;Xi, Zhuge;Lu, Zhisong;...
期刊：
Environmental Science and Pollution Research ,2017年24(2):1784-1793 ISSN：0944-1344
通讯作者：
Yang, Xu;Li, Rui
作者机构：
[Yang, X; Li, R; Li, Jinquan; Li, Rui; Yang, Xu; Zhao, Yun; Shang, Shuai] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Lab Environm Biomed, Wuhan 430079, Hubei Province, Peoples R China.;[Li, Jinquan; Li, Baizhan] Chongqing Univ, Natl Ctr Int Res Low Carbon & Green Bldg, Chongqing 400045, Peoples R China.;[Xi, Zhuge] Inst Hlth & Environm Med, Dali Rd, Tianjin 300050, Peoples R China.;[Lu, Zhisong] Southwest Univ, Inst Clean Energy & Adv Mat, Chongqing 400715, Peoples R China.
通讯机构：
[Yang, X; Li, R] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Lab Environm Biomed, Wuhan 430079, Hubei Province, Peoples R China.
关键词：
Graphene oxide;Allergic asthma;Exacerbation effect;Oxidative stress;Vitamin E
摘要：
Nanomaterials have been widely used in a number of applications; however, these nanomaterials may potentially be risky for human health, particularly for the respiratory system. In this study, we used a mouse asthma model to study whether graphene oxide (GO), a promising carbonaceous nanomaterial with unique physicochemical properties, aggravates allergic asthma via the oxidative stress pathway. Mice were sensitized with ovalbumin (OVA) to trigger immune reactions, while vitamin E (Ve) was administered as an antioxidant. Our results showed that GO aggravated OVA-induced allergic asthma in mice, as suggested by increased reactive oxygen species (ROS), elevated total immunoglobulin E (IgE), upregulated Th2 response, and the aggravation of allergic asthma symptoms, such as airway remolding, collagen deposition with mucus hypersecretion, and airway hyperresponsiveness (AHR). The administration of Ve dramatically attenuated all of the above effects. In conclusion, Ve showed anti-allergic properties in antagonizing the exacerbation of allergic asthma induced by GO, providing a new possibility for the treatment of allergic asthma. © 2016, Springer-Verlag Berlin Heidelberg.
语种：
英文
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Mediating Role of TRPV1 Ion Channels in the Co-exposure to PM2.5 and Formaldehyde of Balb/c Mice Asthma Model
作者：
Song, Jing;Kang, Jun;Lin, Bencheng;Li, Jinquan;Zhu, Yuqing;...
期刊：
Scientific Reports ,2017年7(1):11926 ISSN：2045-2322
通讯作者：
Li, Rui;Xi, Zhuge
作者机构：
[Li, Jinquan; Li, Rui; Yang, Xu; Kang, Jun; Song, Jing; Zhu, Yuqing; Du, Junting] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan 430079, Hubei, Peoples R China.;[Lin, Bencheng; Xi, Zhuge] Tianjin Inst Hlth & Environm Med, Dept Hlth Toxicol, Tianjin 300050, Peoples R China.
通讯机构：
[Li, Rui] C;[Xi, Zhuge] T;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan 430079, Hubei, Peoples R China.;Tianjin Inst Hlth & Environm Med, Dept Hlth Toxicol, Tianjin 300050, Peoples R China.
摘要：
Asthma is a complex pulmonary inflammatory disease that can be promoted by air pollutants such as PM2.5 and formaldehyde (FA). However, existent experimental evidence principally focuses on the negative influence of a single air pollutant, neglecting the possible synergistic effect in biological responses to mixture of these pollutants, a more common situation in our daily life. In this study, allergic Balb/c mice were exposed to a mixture of PM2.5 and FA, and their toxicological effects and mechanisms were explored. It is demonstrated that the combined exposure to PM2.5 and FA can greatly aggravate allergic asthma in mice. When compared with exposure to PM2.5 or FA alone, the co-exposure showed a certain synergistic effect. Increased levels of ROS, inflammatory factors and total serum immunoglobulin E were concomitant with this deterioration. Furthermore, results suggested that co-exposure exacerbated the activation of TRPV1 signal pathways, with an enhancement in substance P and calcitonin gene-related peptide production, which contributed to inflammation in asthma by neurogenic inflammation. The study also proved that capsazepine treatment could reduce the levels of not only pro-inflammatory neuropeptides, but also oxidative stress. It is concluded that co-exposure to PM2.5 and FA exacerbated allergic asthma through oxidative stress and enhanced TRPV1 activation.
语种：
英文
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Functional single-walled carbon nanotubes 'CAR' for targeting dopamine delivery into the brain of parkinsonian mice
作者：
Guo, Qing;You, Huihui;Yang, Xu;Lin, Bencheng;Zhu, Zhihong;...
期刊：
Nanoscale ,2017年9(30):10832-10845 ISSN：2040-3364
通讯作者：
Li, Rui;Xi, Zhuge
作者机构：
[Shen, Shiping; You, Huihui; Yang, Xu; Li, Xiaoxiao; Zhao, Yun; Guo, Qing; Fan, Jianhua; Li, Rui; Deng, Lingfu; Mao, Lin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Lin, Bencheng; Xi, Zhuge] Inst Hlth & Environm Med, Dali Rd, Tianjin 300050, Peoples R China.;[Zhu, Zhihong; Cheng, Haoyan; Zhang, Jiting] Cent China Normal Univ, Coll Phys Sci & Technol, Inst Nanosci & Nanotechnol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Li, Chang Ming; Lu, Zhisong] Southwest Univ, Fac Mat & Energy, Inst Clean Energy & Adv Mat, Tiansheng Rd, Chongqing 400715, Peoples R China.;[Li, Chang Ming] Suzhou Univ Sci & Technol, Inst Mat Sci & Devices, Suzhou 215011, Peoples R China.
通讯机构：
[Li, Rui] C;[Xi, Zhuge] I;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;Inst Hlth & Environm Med, Dali Rd, Tianjin 300050, Peoples R China.
摘要：
Current treatments for Parkinson's disease (PD) are limited, partly due to the difficulties posed by the blood brain barrier (BBB) when delivering drugs to the brain. Herein, we explore the feasibility and efficacy of functional single-walled carbon nanotubes 'CAR' (SWCNT-PEGs-Lf) which carry and target-deliver dopamine (DA) to the brain in PD mice for treatment. SWCNTs can penetrate the cell-membrane remarkably, with the characteristics including high drug-loading and pH-dependent therapeutic unloading capacities. It has been reported that polyethylene glycol (PEG)-coated SWCNTs could increase the circulation time and thus prolong the concentration gradient of SWCNTs to the brain. Besides, an obvious lactoferrin-nanoparticle (Lf-NP) accumulation in the striatum, wherein the pharmacological target site of PD has been reported, a dual modification of PEG and Lf onto SWCNTs was applied and thus a specific 'CAR' to carry DA. The results from in vitro studies demonstrate that with 20 mol L<sup>-1</sup>DA loaded onto SWCNT-polyethylene glycol (PEGs) in addition to 100 μmol L<sup>-1</sup>6-hydroxydopamine (6-OHDA), the activity of PC12 cells increases significantly (p <0.05), and that the lactate dehydrogenase (LDH) levels and reactive oxygen species (ROS) content also significantly decrease (p <0.01). Furthermore, the levels of oxidative stress, tumor necrosis factor (TNF)-αand interleukin (IL)-1βare all reduced significantly in PD mice and the CAR-25 mg kg<sup>-1</sup>DA group in comparison with that in 6-OHDA-lesioned mice with saline and 6-OHDA-lesioned mice, as well as the Tyrosine hydroxylase-immunoreactive (TH-ir) density increased (p <0.01). The toxicity of CAR was in vitro and in vivo investigated, showing that the safe dose of SWCNT-PEG exposure to PC12 cells was 6.25 μg μl<sup>-1</sup>or lower with a higher metabolic activity in comparison with that in the control group and the safe dose of CAR in the mice experiments was 3.25 mg kg<sup>-1</sup>or less, given by intraperitoneal injection with a lower level of oxidative stress and inflammatory responses in comparison with that in the control group. This study suggests that 25 mg kg<sup>-1</sup>DA loaded onto 3.25 mg kg<sup>-1</sup>CAR can alleviate the oxidative stress and inflammatory responses in parkinsonian mice and increase the TH-ir density in the striatum. ©2017 The Royal Society of Chemistry.
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