期刊：
Research in Microbiology,2023年:104173 ISSN：0923-2508
通讯作者：
Yang, Hong
作者机构：
[Qiu, Zhengyong; Mei, Cheng; Wang, Yu] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China;[Shi, Yu] College of Food and Biotechnology, Wuhan Institute of Design and Sciences, Wuhan 430079, PR China;[Yang, Hong] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China. Electronic address: hyang@ccnu.edu.cn
通讯机构：
[Yang, Hong] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China. Electronic address:
摘要：
Verrucomicrobiota is widely distributed in various habitats including insect guts. It was found to be prevalent in almost all investigated termite guts, whereas their physiological functions are not very clear. In this study we characterized the physiological and genomic properties of Verrucomicrobiota strain TSB47(T) isolated from Reticulitermes chinensis. The cells of strain TSB47(T) were Gram-stain-negative, non-motile, and non-spore-forming coccoid with one or more warts. 16S rRNA gene analysis showed that the closest relatives of strain TSB47(T) were Opitutaceae strain TAV1 and Ereboglobus luteus Ho45(T) (98.3% and 95.4% sequence similarity, respectively). Whole genome analysis revealed that there are a large number of glycoside hydrolase genes, amino acid metabolism genes, complete Mo-Fe nitrogenase and Fe-Fe nitrogenase gene clusters, as well as cbb(3)-type cytochrome oxidase gene in the genome of strain TSB47(T). Strain TSB47(T) grows well under anaerobic and microaerophilic conditions with a strong tolerance to oxygen. Physiological and genomic characters of strain TSB47(T) indicated its high adaptability to termite gut ecosystem. Based on phenotypic and phylogenetic evidence, we suggest strain TSB47(T) as the type species of a novel genus in the family Opitutaceae, for which the name Termitidicoccus mucosus sp. nov. is proposed. The type strain is TSB47(T) (CCTCC AB2022447(T); KCTC 102044(T)).
作者机构：
[Sun, Keping; Li, Aoqiang; Li, Zhongle; Leng, Haixia; Feng, Jiang; Jin, Longru] Northeast Normal Univ, Jilin Prov Key Lab Anim Resource Conservat & Utili, Changchun, Peoples R China.;[Li, Aoqiang] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;[Li, Zhongle; Feng, Jiang] Jilin Agr Univ, Coll Life Sci, Changchun, Peoples R China.
通讯机构：
[Keping Sun; Jiang Feng] J;Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, China<&wdkj&>Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, China<&wdkj&>College of Life Science, Jilin Agricultural University, Changchun, China
摘要：
Skin acts as a mechanical barrier between the body and its surrounding environment and plays an important role in resistance to pathogens. However, we still know little regarding skin responses to physiological changes, particularly with regard to responses against potential pathogens. We herein executed RNA-seq on the wing of the Rhinolophus ferrumequinum to assess gene-expression variations at four physiological stages: pre-hibernation, hibernation (early-hibernation and late-hibernation), and post-hibernation, as well as the gene-expression patterns of infected and uninfected bats with the Pseudogymnoascus destructans (Pd). Our results showed that a greater number of differentially expressed genes between the more disparate physiological stages. Functional enrichment analysis showed that the down-regulated response pathways in hibernating bats included phosphorus metabolism and immune response, indicating metabolic suppression and decreased whole immune function. We also found up-regulated genes in post-hibernating bats that included C-type lectin receptor signalling, Toll-like receptor signalling pathway, and cell adhesion, suggesting that the immune response and skin integrity of the wing were improved after bats emerged from their hibernation and that this facilitated clearing Pd from the integument. Additionally, we found that the genes involved in cytokine or chemokine activity were up-regulated in late-hibernation compared to early-hibernation and that FOSB regulation of immune cell activation was differentially expressed in bats infected with Pd during late-hibernation, implying that the host's innate immune function was enhanced during late-hibernation so as to resist pathogenic infection. Our findings highlight the concept that maintenance of intrinsic immunity provides protection against pathogenic infections in highly resistant bats.
摘要：
As hotspots for the dissemination of antibiotic resistance genes (ARGs), wastewater treatment plants (WWTPs) have attracted global attention. However, there lacks a sufficient metagenomic surveillance of antibiotic resistome in the WWTPs located on the Qinghai-Tibet Plateau. Here, metagenomic approaches were used to comprehensively investigate the occurrence, mobility potential, and bacterial hosts of ARGs in influent and effluent of 18 WWTPs located on the Qinghai-Tibet Plateau. The total ARG relative abundances and diversity were significantly decreased from influent to effluent across the WWTPs. Multidrug, bacitracin, sulfonamide, aminoglycoside, and beta-lactam ARGs generally consisted of the main ARG types in effluent samples, which were distinct from influent samples. A group of 72 core ARGs accounting for 61.8-95.8 % of the total ARG abundances were shared by all samples. Clinically relevant ARGs mainly conferring resistance to beta-lactams were detected in influent (277 ARGs) and effluent (178 ARGs). Metagenomic assembly revealed that the genetic location of an ARG on a plasmid or a chromosome was related to its corresponding ARG type, demonstrating the distinction in the mobility potential of different ARG types. The abundance of plasmid-mediated ARGs accounted for a much higher proportion than that of chromosome-mediated ARGs in both influent and effluent. Moreover, the ARGs co-occurring with diverse mobile genetic elements in the effluent exhibited a comparable mobility potential with the influent. Furthermore, 137 metagenome-assembled genomes (MAGs) assigned to 13 bacterial phyla were identified as the ARG hosts, which could be effectively treated in most WWTPs. Notably, 46 MAGs were found to carry multiple ARG types and the potential pathogens frequently exhibited multi-antibiotic resistance. Some ARG types tended to be carried by certain bacteria, showing a specific host-resistance association pattern. This study highlights the necessity for metagenomic surveillance and will facilitate risk assessment and control of antibiotic resistome in WWTPs located on the vulnerable area.
作者机构：
[周敏] Geriatric Department, Chongqing Emergency Medical Center, The Fourth People's Hospital of Chongqing, Chongqing, 400030, China;[郑子光; 游宏宇] Western Investment Construction Co., LDT of CCTEB, Chengdu, 610041, China;[郭淼; 喻伟] School of Civil Engineering, Chongqing University, Chongqing, 400045, China;[杨旭] School of Life Sciences, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Yu, W.] S;School of Civil Engineering, China
作者机构：
[Zhou, Cheng; Liu, Ying; Yang, Shuaikang; Zang, Jianfeng; Liu, Xurui; Tian, Ye; Tang, Hanchuan] Huazhong Univ Sci & Technol, Sch Integrated Circuits, Wuhan 430074, Peoples R China.;[Zhou, Cheng; Liu, Ying; Kang, Tianyu; Yang, Shuaikang; Zang, Jianfeng; Liu, Xurui; Tian, Ye; Tang, Hanchuan] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China.;[Chen, Wei; Zhang, Shujie] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;[Chen, Qicai] Cent China Normal Univ, Sch Life Sci, Wuhan 430074, Peoples R China.;[Xiao, Hongjun] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Otorhinolaryngol, Wuhan 430022, Peoples R China.
通讯机构：
[Hongjun Xiao; Hongjun Xiao Hongjun Xiao Hongjun Xiao] D;[Wei Chen; Wei Chen Wei Chen Wei Chen] C;[Jianfeng Zang; Jianfeng Zang Jianfeng Zang Jianfeng Zang] S;College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 China<&wdkj&>School of Integrated Circuits and Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074 China<&wdkj&>The State Key Laboratory of Digital Manufacturing Equipment and Technology, Huazhong University of Science and Technology, Wuhan, 430074 China<&wdkj&>Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022 China
摘要：
A bioinspired soft elastic metamaterial with a size equivalent to the current cochlear implant that can be seen as a prototype of passive artificial cochlea with a frequency resolution of 30 Hz is proposed. This work will inspire future work in the fabrication of totally implantable passive artificial cochlea that enables the users to hear naturally. Abstract Natural hearing which means hearing naturally like normal people is critical for patients with hearing loss to participate in life. Cochlear implants have enabled numerous severe hearing loss patients to hear voice functionally, while cochlear implant users can hardly distinguish different tones or appreciate music subject to the absence of rate coding and insufficient frequency channels. Here a bioinspired soft elastic metamaterial that reproduces the shape and key functions of the human cochlea is reported. Inspired by human cochlea, the metamaterials are designed to possess graded microstructures with high effective refractive index distributed on a spiral shape to implement position‐related frequency demultiplexing, passive sound enhancements of 10 times, and high‐speed parallel processing of 168‐channel sound/piezoelectric signals. Besides, it is demonstrated that natural hearing artificial cochlea has fine frequency resolution up to 30 Hz, a wide audible range from 150–12 000 Hz, and a considerable output voltage that can activate the auditory pathway in mice. This work blazes a promising trail for reconstruction of natural hearing in patients with severe hearing loss.
期刊：
Journal of Advanced Research,2023年48:105-123 ISSN：2090-1232
通讯作者：
Xie, Na;Huang, CH;Chen, MQ
作者机构：
[Huang, Canhua; Xie, Na; Huang, CH; Duan, Jiufei; Huang, Zhao] West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China.;[Huang, Canhua; Xie, Na; Huang, CH; Duan, Jiufei; Huang, Zhao] West China Hosp, Canc Ctr, Chengdu 610041, Peoples R China.;[Huang, Canhua; Xie, Na; Huang, CH; Duan, Jiufei; Huang, Zhao] Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Chengdu 610041, Peoples R China.;[Huang, Canhua; Xie, Na; Huang, CH; Duan, Jiufei; Huang, Zhao] Collaborat Innovat Ctr Biotherapy, Chengdu 610041, Peoples R China.;[Nice, Edouard C.] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic, Australia.
通讯机构：
[Chen, MQ ] C;[Xie, N; Huang, CH ] W;West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China.;West China Hosp, Canc Ctr, Chengdu 610041, Peoples R China.;Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Chengdu 610041, Peoples R China.
关键词：
Long noncoding RNAs;Lipid metabolism;Lipid chaperones;Lipid receptors;Therapeutic target
摘要：
Background: The investigation of lncRNAs has provided a novel perspective for elucidating mechanisms underlying diverse physiological and pathological processes. Compelling evidence has revealed an intrin-sic link between lncRNAs and lipid metabolism, demonstrating that lncRNAs-induced disruption of lipid metabolism and signaling contribute to the development of multiple cancers and some other diseases, including obesity, fatty liver disease, and cardiovascular disease.Aimof Review: The current review summarizes the recent advances in basic research about lipid metabo-lism and lipid signaling-related lncRNAs. Meanwhile, the potential and challenges of targeting lncRNA for the therapy of cancers and other lipid metabolism-related diseases are also discussed.Key Scientific Concept of Review: Compared with the substantial number of lncRNA loci, we still know little about the role of lncRNAs in metabolism. A more comprehensive understanding of the function and mechanism of lncRNAs may provide a new standpoint for the study of lipid metabolism and signaling. Developing lncRNA-based therapeutic approaches is an effective strategy for lipid metabolism-related diseases.(c) 2023 Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
期刊：
JOURNAL OF EXPERIMENTAL BOTANY,2023年74(6):1836-1852 ISSN：0022-0957
通讯作者：
Yang Li<&wdkj&>Xue-Bao Li
作者机构：
[Wang, Yao; Li, Yu; Zheng, Yong; Li, Xue-Bao; Zhang, Shi-Peng; Li, Yang; Cheng, Fan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Yang Li; Xue-Bao Li] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University , Wuhan 430079 , China
摘要：
Cotton fiber elongation is a critical growth phase that affects final fiber length. Morphological analysis indicated an asynchronous fiber elongation pattern between two cotton varieties, J7-1 and J14-1. Through phosphoproteomic analysis, a total of 89 differentially-phosphorylated proteins (DPPs) were identified in elongating fibers between J7-1 and J14-1. Gene ontology (GO) analysis showed that these DPPs were mainly enriched in sucrose synthase activity, transferase activity, and UDP-glycosyltransferase activity. In J14-1, the phosphorylation level of GhSUS2, a key sucrose synthase in the sucrose metabolism pathway, was significantly higher than that in J7-1. We further revealed that GhSUS2 positively regulates fiber elongation, and GhSUS2-silenced transgenic cotton displayed the phenotype of 'short fibers' compared with the controls. During fiber development, the residue Ser11 in the GhSUS2 protein is phosphorylated by the Ca2+-dependent protein kinases GhCPK84 and GhCPK93. Phosphorylated GhSUS2 is localized in the cytoplasm, whereas unphosphorylated GhSUS2 is localized in the plasma membrane. Moreover, abscisic acid (ABA) could promote the transcription and translation of GhCPK84 and GhCPK93, thereby enhancing the phosphorylation of GhSUS2 to impede fiber elongation. Thus, our data demonstrates that GhSUS2 plays a positive role in fiber development, but its phosphorylation by GhCPK84 and GhCPK93 hinders fiber elongation of cotton.
作者机构：
[Chen, Mingqing; Han, Qi; Wei, Zhaolan; Deng, Lingfu; Wang, Yunyi; Wang, Shuwei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Mingqing] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Chen, Mingqing] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China. Electronic address:
摘要：
Previous studies suggest some link between formaldehyde exposure and harmful cardiovascular effects. But whether exposure to formaldehyde can cause blood pressure to rise, and if so, what the underlying mechanism is, remains unclear. In this study, C57BL/6 male mice were exposed to 0.1, 0.5, 2.5mg/m(3) of gaseous formaldehyde for 4hours daily over a three-week period. The systolic blood pressure (SBP), diastolic blood pressure (DBP), mean blood pressure (MBP) and heart rate (HR) of the mice were measured by tail-cuff plethysmography, and any histopathological changes in the target organs of hypertension were investigated. The results showed that exposure to formaldehyde did cause a significant increase in blood pressure and heart rate, and resulted in varying degrees of damage to the heart, aortic vessels and kidneys. To explore the underlying mechanism, a specific inhibitor of angiotensin converting enzyme (ACE) was used to block the ACE/AT1R axis. We observed the levels of ACE and angiotensin II type 1 receptor (AT1R), as well as the bradykinin (BK) in cardiac cytoplasm. The data suggest that exposure to formaldehyde induced an increase in the expression of ACE and AT1R, and decreased the levels of BK. Strikingly, treatment with 5mg/kg/d ACE inhibitor can attenuate the increase in blood pressure and the pathological changes caused by formaldehyde exposure. This result has improved our understanding of whether, and how, formaldehyde exposure affects the development of hypertension.
摘要：
West of Beijing, Chinese white‐bellied rats (Niviventer confucianus, CWR) and Korean field mice (Apodemus peninsulae, KFM) are common and share similar habitat, diet, and activity, but differ in body size (CWR are bigger than KFM), food hoarding habit, and ability to protect caches. Intense asymmetric competition for food exists between the 2 rodent species, in that CWR have distinct advantage than KFM. However, how KFM coexists with CWR is less known. By tracking seed competition of 15 pairs of CWR–KFM over a 10‐day period for each under enclosure conditions, we found KFM could not compete over CWR at the seed source, but in terms of relative number (seed number/2) and relative energy of seeds (seed energy/3), they hoarded more seeds than CWR at the end of the trials. These results may promote their coexistence under the conditions of asymmetric competition. Abstract Asymmetric competition occurs when some species have distinct advantages over their competitors and is common in animals with overlapping habitats and diet. However, the mechanism allowing coexistence between asymmetric competitors is not fully clear. Chinese white‐bellied rats (Niviventer confucianus, CWR) and Korean field mice (Apodemus peninsulae, KFM) are common asymmetric competitors in shrublands and forests west of Beijing city. They share similar diet (e.g. plant seeds) and activity (nocturnal), but differ in body size (CWR are bigger than KFM), food hoarding habit (CWR: mainly larder hoarding; KFM: both larder and scatter hoarding), and ability to protect cached food (CWR are more aggressive than KFM). Here, we tested seed competition in 15 CWR–KFM pairs over a 10‐day period under semi‐natural enclosure conditions to uncover the differences in food hoarding, cache pilferage, and food protection between the 2 rodents, and discuss the implication for coexistence. Prior to pilferage, CWR harvested and ate more seeds than KFM. CWR tended to larder hoard seeds, whereas KFM preferred to scatter hoard seeds. Following pilferage, CWR increased consumption, decreased intensity of hoarding, and pilfered more caches from KFM than they lost, while KFM increased consumption more than they hoarded, and they preferred to hoard seeds in low and medium competition areas. Accordingly, both of the 2 rodent species increased their total energy consumption and hoarding following pilferage. Both rodent species tended to harvest seeds from the source, rather than pilfer caches from each other to compensate for cache loss via pilferage. Compared to CWR, KFM consumed fewer seeds when considering seed number, but hoarded more seeds when considering the seeds’ relative energy (energy of hoarded seeds/rodent body mass2/3) at the end of the trials. These results suggest that asymmetric competition for food exists between CWR and KFM, but differentiation in hoarding behavior could help the subordinate species (i.e. KFM) hoard more energy than the dominant species (i.e. CWR), and may contribute to their coexistence in the field.
作者机构：
[Huang, Shuang-Quan; Feng, Hui-Hui] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Wang, Xiao-Yue] Guizhou Normal Univ, Sch Life Sci, Key Lab State Forestry Adm Biodivers Conservat Ka, Guiyang 550025, Peoples R China.;[Luo, Yi-Bo] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China.
通讯机构：
[Shuang-Quan Huang; Shuang-Quan Huang Shuang-Quan Huang Shuang-Quan Huang] I;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan, 430079 China
关键词：
floral color change;floral scent change;Linalool;Lonicera japonica;nectar dynamics;scent emission rhythm.
摘要：
Flowers in the gold‐and‐silver honeysuckle (Lonicera japonica Thunb.) are white on opening at night (N1) and become light yellow the following day (D1), yellow on the second night (N2), and golden on the second day of flowering (D2). Our measurements in the four stages of anthesis indicated that nectar production decreased significantly from N1 and D1 to N2 and D2, tracking the floral color change. However, both total scent emission of floral scent and scent emission of three major compounds were significantly higher in N2 than in the other three stages, suggesting that floral scent does not always co‐vary with floral color. Abstract Floral color change in diverse plants has been thought to be a visual signal reflecting changes in floral rewards, promoting pollinator foraging efficiency as well as plant reproductive success. It remains unclear whether olfactory signals co‐vary with floral color change. We investigated the production rhythms of floral scent and nectar associated with floral color change in Lonicera japonica. The flowers generally last 2–3 days. They are white on opening at night (N1) and become light yellow the following day (D1), yellow on the second night (N2), and golden on the second day of flowering (D2). Our measurements in the four stages indicated that nectar production decreased significantly from N1 and D1 to N2 and D2, tracking the floral color change. A total of 34 compounds were detected in floral scent and total scent emission was significantly higher in N2 than in the other three stages. The scent emission of three major compounds, Linalool, cis‐3‐Hexenyl tiglate, and Germacrene D was also significantly higher in N2, but the relative content of Linalool decreased gradually, cis‐3‐Hexenyl tiglate increased gradually, and the relative content of Germacrene D did not differ among the four measured stages. Greater scent emission by night than by day suggested a strong olfactory signal to attract nocturnal hawkmoths, the effective pollinators. However, floral scent rhythms in the four stages did not match the color change and nectar secretion, suggesting that floral color (visual) and scent (olfactory) in this species may play different roles in attracting or filtering various visitors.
通讯机构：
[Rong Peng] I;Institute of Entomology, School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
fluorescent nanoparticle;RNA interference;sterol carrier protein-2;Helicoverpa armigera;growth and development;pest control
摘要：
Helicoverpa armigera is a polyphagous destructive lepidopteran pest with strong Bacillus thuringiensis (Bt) resistance. Cholesterol, a vital component for insect growth, can only be obtained from food, and its transfer and metabolism are regulated by sterol carrier protein-2 (SCP-2). This study examined whether H. armigera SCP-2 (HaSCP-2) gene expression, involved in cholesterol absorption, can be silenced by nanocarrier fluorescent nanoparticle-RNA interference (FNP-RNAi) by larval feeding and whether the silencing affected H. armigera development. Fluorescence microscopy showed that nanoparticle-siRNA was distributed in Ha cells and the larval midgut. FNP-HaSCP-2 siRNA suppressed HaSCP-2 expression by 52.5% in H.armigera Ha cells. FNP can effectively help deliver siRNA into cells, protect siRNA, and is not affected by serum. FNP-siRNA in vivo biological assays showed that HaSCP-2 transcript levels were inhibited by 70.19%, 68.16%, and 67.66% in 3rd, 4th, and 5th instar larvae, leading to a decrease in the cholesterol level in the larval and prepupal fatbodies. The pupation rate and adult emergence were reduced to 26.0% and 56.52%, respectively. This study demonstrated that FNP could deliver siRNA to cells and improve siRNA knockdown efficiency. HaSCP-2 knockdown by FNP-siRNA in vivo hindered H. armigera growth and development. FNP could enhance RNAi efficiency to achieve pest control by SCP-2-targeted FNP-RNAi.
作者机构：
[Cheng, Ying; Liao, Chongyu; Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr,Genome Anal Lab, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr, Shenzhen 518120, Peoples R China.;[Wu, Kongming; Zhang, Dandan] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100089, Peoples R China.;[Liu, Kaiyu; Yang, Yongbo] Cent China Normal Univ, Coll Life Sci, Wuhan, Peoples R China.
通讯机构：
[Kongming Wu; Kongming Wu Kongming Wu Kongming Wu] T;[Yutao Xiao; Yutao Xiao Yutao Xiao Yutao Xiao] S;The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China<&wdkj&>Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
摘要：
Evolution of resistance to Cry proteins in multiple pest insects has been threatening the sustainable use of Bacillus thuringiensis (Bt)-transgenic crops. Better understanding about the mechanism of resistance to Cry proteins in insects is needed. Our preliminary study reported that the transcription of HaABCC3 was significantly decreased in a near-isogenic line (LFC2) of a Cry1Ac-resistant strain (LF60) of the global pest Helicoverpa armigera. However, the causality between HaABCC3 down-regulation and resistance to Cry1Ac remains to be verified, and the regulatory mechanism underlying the HaABCC3 down-regulation is still unclear. In this study, our data showed that both HaABCC3 and HaABCC3 down-regulation were genetically linked to resistance to Cry1Ac in LF60. However, no InDels were observed in the coding sequence of HaABCC3 from LF60. Furthermore, F1 offspring from the cross of LF60 and a HaABCC2/3-knockout (KO) mutant exhibited moderate resistance to Cry1Ac toxin; this indicated that the high resistance to Cry1Ac toxin in LF60 may have resulted from multiple genetic factors, including HaABCC2 mis-splicing and HaABCC3 down-regulation. Results from luciferase reporter assays showed that promoter activity of HaABCC3 in LF60 was significantly lower than that in the susceptible strain, which indicated that HaABCC3 down-regulation was likely mediated by promoter variation. Consistently, multiple variations of the GATA- or FoxA-binding sites in the promoter region of HaABCC3 were identified. Collectively, all results in this study suggested that down-regulation of HaABCC3 observed in the H. armigera LF60 strain, that is resistant to Cry1Ac, may be mediated by a cis-regulatory mechanism. Down-regulation of HaABCC3 was genetically linked with resistance to Cry1Ac toxin. Offspring from cross of LF60 and HaABCC2/3-KO exhibited resistance to Cry1Ac toxin. Down-regulation of HaABCC3 was potentially mediated by cis-regulatory mechanism. This article is protected by copyright. All rights reserved.
摘要：
This study investigated whether using multiwalled carbon nanotubes (MWCNTs) as a carrier for dibutyl phthalate (DBP) could delay the degradation rate of DBP in mice and increase its estrogen-like interference effect. Pregnant Balb/C mice were divided into four groups and exposed to different treatments via tail-vein injection every 3 days until gestational day 20. The female and male mice were then sacrificed for toxicological study. The results showed that the combination of MWCNTs and DBP resulted in a higher fetal mortality rate than if the mice were exposed to MWCNTs or DBP alone. H & E staining showed that the estrous period of the exposed mice was delayed, the development of oocytes was blocked in the combination group, the number of spermatogenic cells decreased, and the quality of sperm decreased. Our experiment showed that the expression levels of the genes involved in sex hormone synthesis in the testis and ovaries were significantly increased after combined treatment compared with the MWCNT group (p < 0.01). The study suggests that DBP degradation is delayed when absorbed on MWCNTs, which increases its estrogen-like interference and interferes with fetal development, ultimately leading to increased fetal mortality.
