作者： Xie, Xiaoman;Deng, Ting;Duan, Jiufei;Xie, Jing;Yuan, Junlin;...

期刊： Ecotoxicology and Environmental Safety,2020年190:110133 ISSN：0147-6513

通讯作者： Chen, Mingqing

作者机构： [Xie, Jing; Yuan, Junlin; Chen, Mingqing; Duan, Jiufei; Deng, Ting; Xie, Xiaoman] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Mingqing] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.

通讯机构： [Chen, Mingqing] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.

关键词： MAPK signaling pathway;Microplastics;Oxidative stress;Polystyrene microplastics;Reproductive toxicity

摘要： Microplastics (MP) are receiving increased attention as a harmful environmental pollutant, however information on the reproduction toxicity of MP in terrestrial animals, especially mammals, is limited. In this experiment, we investigated the impact of polystyrene microplastics (micro-PS) on the reproductive system of male mice. Healthy Balb/c mice were exposed to saline or to different doses of micro-PS for 6 weeks. The results showed that micro-PS exposure resulted in a significant decrease in the number and motility of sperm, and a significant increase in sperm deformity rate. We also detected a decrease in the activity of the sperm metabolism-related enzymes, succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), and a decrease in the serum testosterone content in the micro-PS exposure group. We found that micro-PS exposure caused oxidative stress and activated JNK and p38 MAPK. In addition, we found that when N-acetylcysteine (NAC) scavenges ROS, and when the p38 MAPK-specific inhibitor SB203580 inhibits p38MAPK, the micro-PS-induced sperm damage is alleviated and testosterone secretion improves. In conclusion, our findings suggest that micro-PS induces reproductive toxicity in mice through oxidative stress and activation of the p38 MAPK signaling pathways. © 2019 Elsevier Inc.

语种： 英文

作者： Xie, Xiaoman;Deng, Ting;Duan, Jiufei;Ding, Shumao;Yuan, Junlin*;...

期刊： Ecotoxicology and Environmental Safety,2019年174:75-82 ISSN：0147-6513

通讯作者： Yuan, Junlin;Chen, Mingqing

作者机构： [Yuan, JL; Chen, Mingqing; Yuan, Junlin; Duan, Jiufei; Deng, Ting; Xie, Xiaoman; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

通讯机构： [Yuan, JL; Chen, MQ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

关键词： DBP;DEHP;Estradiol;Hypertension;RAAS

摘要： Epidemiological studies have shown that high molecular weight phthalates (HMW) such as diethylhexyl phthalate (DEHP), are associated with hypertension in humans, while low molecular weight phthalates (LMW) such as dibutyl phthalate (DBP), have hardly any impact on the elevation of blood pressure. However, the molecular mechanisms responsible for this difference are not completely understood. In this experiment, mice were exposed to 0.1/1/10 mg/kg/day DEHP and 0.1/1/10 mg/kg/day DBP for 6 weeks, and their blood pressure was monitored using the tail pressure method. The results showed that exposure to DEHP dosages of 1 or 10 mg/kg/day resulted in a sharp increase in blood pressure, while exposure to DBP did not induce any significant changes in blood pressure. Investigating the renin-angiotensin-aldosterone system (RAAS) and NO pathway in mice exposed to DEHP, we found that levels of angiotensin-converting enzyme (ACE) and angiotensin II (AngII) increased with increasing exposure to DEHP, and the expression of nitric oxide synthase (eNOS) and the level of NO decreased. Treatment with ACE inhibitor (ACEI) to block the ACE pathway inhibited the enhancement of RAAS expression, inhibited the increase in blood pressure, and inhibited the decrease in NO levels induced by DEHP. However, the expression of ACE, AngII, AT1R, and eNOS in the DBP treatment groups showed no significant changes. When examining estradiol in vivo, we found that exposure to DBP resulted in a significant increase in the level of estradiol, while exposure to DEHP did not lead to a significant change. When ICI182780 was used to block the estradiol receptors, any increase in the level of NO induced by DBP exposure, was inhibited. These results indicate that exposure to DEHP induces an increase in mouse blood pressure through RAAS, and the different effects of DEHP and DBP on blood pressure are partly due to the different estradiol levels induced by DEHP and DBP. © 2019 Elsevier Inc.

语种： 英文

作者： 张倩;杜俊停;黄佳伟;崔海燕;路曼曼;...

期刊： 环境科学学报,2019年39(2):633-639 ISSN：0253-2468

通讯作者： Yuan, J.

作者机构： 华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉430079;[路曼曼; 黄佳伟; 袁均林; 陈明清; 李睿; 张倩; 丁书茂; 杜俊停; 崔海燕] 华中师范大学

通讯机构： Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China

关键词： 三氟羧草醚;学习记忆;氧化应激

摘要： 目前,有关三氟羧草醚( Acifluorfen,AF)的神经毒性未见报道且亟待确定.为探讨AF经口暴露对小鼠学习记忆能力的影响及其可能机制,将30只雄性昆明小鼠随机分成生理盐水对照组、0.13、1.3、13和130 mg·kg~(-1)·d~(-1) AF染毒组共5组,灌胃染毒14 d,进行Morris水迷宫实验,观察脑海马病理切片,并检测脑组织中活性氧( ROS) 、丙二醛( MDA) 、还原型谷胱甘肽( GSH) 、磷酸化cAMP反应元件结合蛋白( pCREB)和脑源性神经营养因子( BDNF)含量.结果显示,与对照组相比,AF染毒剂量分别为13和130 mg·kg~(-1)·d~(-1)时,小鼠行为学上学习记忆能力下降;海马细胞排列松散;出现氧化损伤,其中,ROS含量显著升高( p<0.05) ,GSH含量显著减少( p<0.05) ;神经保护能力减弱,其中,pCREB和BDNF水平显著( p<0.05)或极显著( p<0.01)下降.结果表明,AF能够导致小鼠学习记忆能力下降,氧化应激和CREB-BDNF通路级联下调可能是AF造成神经毒性的机制之一.

语种： 中文

作者： Zhang, Qian;Liu, Zhimin;Du, Junting;Qin, Wei;Lu, Manman;...

期刊： JOURNAL OF TOXICOLOGICAL SCIENCES,2019年44(1):35-45 ISSN：0388-1350

通讯作者： Yuan, Junlin

作者机构： [Lu, Manman; Yuan, Junlin; Liu, Zhimin; Li, Rui; Li, Xiaoxiao; Zhang, Qian; Cui, Haiyan; Du, Junting; Qin, Wei; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

通讯机构： [Yuan, Junlin] C;Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

关键词： Nano-titanium dioxide (nano-TiO2);Cardiovascular toxicity;Oxidative stress;Inflammation;Cytotoxicity;Apoptosis

摘要： Due to its excellent properties such as ultraviolet obscuration, chemical stability and small particle size, nano-titanium dioxide (nano-TiO 2 ) is widely used, particularly in sunblock products. The skin is therefore a chief route for exposure. Studies have found that oral or respiratory exposure to nano-TiO 2 has an adverse impact on the cardiovascular system. The relationship between dermal exposure to nano-TiO 2 and cardiovascular system toxicity, particularly the causative mechanisms, remain unclear. In this study, we used Balb/c mice to evaluate cardiovascular toxicity from nano-TiO 2 dermal exposure, and the underlying mechanisms associated with the human umbilical vein endothelial cells (HUVECs) were explored. Our results showed that nano-TiO 2 treatment resulted in an obvious increase in reactive oxygen species and 8-hydroxy-2’-deoxyguanosine, indicating the appearance of oxidative stress. Moreover, the levels of inflammatory biomarkers, such as immunoglobulin E, soluble intercellular adhesion molecule-1, interleukin-8, and hypersensitive C-reactive protein, also increased. Exposing HUVECs to nano-TiO 2 led to a decline in cell vitality, and an increase in caspase-3 levels, suggesting that nano-TiO 2 exposure caused cytotoxicity and even cell apoptosis. Interestingly, neutralizing oxidative stress by administering Vitamin E was shown to reduce the inflammatory response and cytotoxicity. Our findings suggest that nano-TiO 2 can injure the cardiovascular system via dermal exposure, and does this via oxidative stress-induced inflammation and cytotoxicity. Vitamin E treatment may be a strategy to mitigate the damage. © 2019, Japanese Society of Toxicology. All rights reserved.

语种： 英文

作者： Li, Xiaoxiao;Zhao, Yun;Ding, Shumao;You, Huihui;Feng, Weiyong;...

期刊： Food and Agricultural Immunology,2018年29(1):267-280 ISSN：0954-0105

通讯作者： Yuan, Junlin

作者机构： [Yuan, Junlin; Li, Xiaoxiao; You, Huihui; Yang, Xu; Zhao, Yun; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luo Yu Rd, Wuhan 430079, Hubei, Peoples R China.;[Feng, Weiyong] Cent China Normal Univ, Sch Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China.

通讯机构： [Yuan, Junlin] C;Cent China Normal Univ, Sch Life Sci, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luo Yu Rd, Wuhan 430079, Hubei, Peoples R China.

关键词： Analysis;ELISA;Polyclonal antibody;Thifluzamide;Two formats

摘要： Haptens 2-Methyl-4-(trifluoromethyl)thiazole-5-carboxylic acid and 2,6-Dibromo-4-(trifluoromethoxy)aniline, the two moieties of thifluzamide, were conjugated with carrier proteins for the synthesis of artificial antigens. Two distinct anti-thifluzamide polyclonal antibodies (PAb-1 and PAb-2) were produced from the immunized female Balb/c mice. The indirect competitive enzymelinked immunosorbent assay (ic-ELISA) in two formats based on the PAbs was developed for thifluzamide analysis. The concentration of 50% inhibition (IC 50 ) of ELISA-1 was 1.39 mg L −1 and its limit of detection (LOD) was 0.082 mg L −1 . Meanwhile, ELISA-2 had a similar IC 50 of 1.96 mg L −1 and a LOD of 0.074 mg L −1 as ELISA-1. Both the raised PAbs exhibited high specificity to thifluzamide. The recoveries for spiked samples including water and wheat ranged from 72.0% to 128.4%, and the accuracy of ELISA was confirmed by high-performance liquid chromatography. In summary, the ic-ELISA might be a promising tool for simple, sensitive and rapid detection of thifluzamide residues in real samples. © 2017 The Author(s).

语种： 英文

作者： Li Xiao-Xiao;Ding Shu-Mao;Yang Xu;Yuan Jun-Lin*

期刊： 分析化学,2018年46(1):27-32 ISSN：0253-3820

通讯作者： Yuan Jun-Lin

作者机构： [Ding Shu-Mao; Yuan Jun-Lin; Yang Xu; Li Xiao-Xiao] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

通讯机构： [Yuan Jun-Lin] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

关键词： 噻呋酰胺;人工抗原;多克隆抗体;酶联免疫吸附分析

摘要： 以化合物2-甲基-4-三氟甲基-5-噻唑甲酸(MTCA)为噻呋酰胺的半抗原合成人工抗原,采用活泼酯法合成免疫原MTCA-BSA,分别采用活泼酯法、混合酸酐法和N,N'-羰基二咪唑/4-二甲氨基吡啶(CDI / DMAP)法合成3种包被原MTCA-OVA-1、MTCA-OVA-2和MTCA-OVA-3。免疫动物后,最终以包被原MTCAOVA- 3筛选并获得了具有高特异性的噻呋酰胺多克隆抗体,建立了检测噻呋酰胺的间接竞争酶联免疫吸附(ic-ELISA)分析方法。本方法线性检测范围为0.08 ~ 10.00 mg /L,半数抑制浓度(IC_(50))为1.39 mg /L,检出限为0.08 mg /L,对自来水、湖水和小麦中的噻呋酰胺添加回收率在72.0%~ 128.3%之间,检测结果与HPLC法具有良好的相关性(R~2 = 0.9994) 。本研究建立的ic-ELISA方法可用于环境水样与小麦等农产品中噻呋酰胺残留的快速检测。

语种： 中文

作者： Du, Junting;Xiong, Di;Zhang, Qian;Li, Xiaoxiao;Liu, Xudong;...

期刊： JOURNAL OF TOXICOLOGICAL SCIENCES,2017年42(3):319-328 ISSN：0388-1350

通讯作者： Yuan, Junlin

作者机构： [Yuan, Junlin; Li, Xiaoxiao; You, Huihui; Yang, Xu; Zhang, Qian; Xiong, Di; Du, Junting; Liu, Xudong; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

通讯机构： [Yuan, Junlin] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.

关键词： Mono-butyl phthalate;Testicular toxicity;Oxidative stress;Sox9;Dazl

摘要： Mono-butyl phthalate (MBP) has reproductive toxicity but the related mechanisms have not been fully elucidated in vivo. We exposed male Balb/c mice to MBP lby gavage at doses of 0, 25, 50, 100, 200 mg/kg for 14 days, and then evaluated the testicular alterations at the histological and molecular levels. MBP reduced mouse sperm count along with sperm malformation and seminiferous tubule degeneration in a dose-dependent manner. MBP dosed at 200 mg/kg significantly increased reactive oxygen species and malondialdehyde content in mouse testes. High doses of MBP (200 mg/kg) also significantly reduced mRNA expressions of testis growth and function related genes (Sox9 and Dazl). Our findings suggest that oxidative stress and down-regulated expression of Sox9 and Dazl may play important roles in MBP-induced testis injury. © 2017, Japanese Society of Toxicology. All rights reserved.

语种： 英文

作者： Wei, Chenxi;Chen, Mouying;You, Huihui;Qiu, Feng;Wen, Huaxiao;...

期刊： Toxicology and Applied Pharmacology,2017年324(2):36-44 ISSN：0041-008X

通讯作者： Xiang, Shuanglin;Yang, Xu

作者机构： [Chen, Mouying; Wei, Chenxi; Qiu, Feng; Xiang, Shuanglin] Hunan Normal Univ, Sch Life Sci, Key Lab Ecol Safety Monitoring & Evaluat, Changsha 410081, Hunan, Peoples R China.;[Wei, Chenxi; Yuan, Junlin; You, Huihui; Wen, Huaxiao; Yang, Xu] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan 430079, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Sch Life Sci, 152 Luo Yu Rd, Wuhan 430079, Hubei, Peoples R China.;[Xiang, Shuanglin] Hunan Normal Univ, Sch Life Sci, 36 Lu Shan Rd, Changsha 410081, Hunan, Peoples R China.

通讯机构： [Xiang, Shuanglin] H;[Yang, Xu] C;Hunan Normal Univ, Sch Life Sci, 36 Lu Shan Rd, Changsha 410081, Hunan, Peoples R China.;Cent China Normal Univ, Sch Life Sci, 152 Luo Yu Rd, Wuhan 430079, Hubei, Peoples R China.

关键词： *Benzene;*Bone marrow;*Formaldehyde;*Hematotoxicity;*Myeloid progenitor;*Post-exposure

摘要： Formaldehyde (FA) is a human leukemogen. Since there is a latency period between initial FA exposure and the development of leukemia, the subsequent impact of FA on hematopoietic stem or progenitor cells (HSCs/HPCs) in post-exposure stage is crucial for a deep understanding of FA-induced hematotoxicity. BALB/c mice were exposed to 3 mg/m(3) FA for 2 weeks, mimicking occupational exposure, and were monitored for another 7 days post-exposure. Meanwhile, we included benzene (BZ) as a positive control, separately and together with FA because co-exposure occurs frequently. After 7-day recovery, colonies of progenitors for CFU-GM and BFU-E, and nucleated bone marrow cells in FA-exposed mice were comparable to controls, although they were significantly reduced during exposure. Levels of reactive oxygen species (ROS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in CFU-GM and BFU-E from FA-exposed mice were higher than controls, although the increase in 8-OHdG was not significant. Granulocyte-macrophage colony stimulating factor (GM-CSF) level in the FA group was lower than controls, but the expression level for the receptor was not upregulated. It suggests that HSCs/HPCs in FA-exposed mice respond to a small amount of GM-CSF and proliferate rapidly, which may cause a possible risk of expansion of abnormal stem/progenitor cell clones. FA co-exposure with BZ was more potent for promoting CFU-GM formation and inducing ROS in BFU-E and 8-OHdG in CFU-GM during the post-exposure period. The compensation of myeloid progenitors with elevated ROS and 8-OHdG may lead to a risk of transforming normal HSCs/HPCs to leukemic stem/progenitor cells. Thus, co-exposure may pose a greater leukemia risk. (C) 2017 Elsevier Inc. All rights reserved.

语种： 英文

作者： Wei, Chenxi;Wen, Huaxiao;Yuan, Langyue;McHale, Cliona M.;Li, Hui;...

期刊： Archives of Toxicology,2017年91(2):921-933 ISSN：0340-5761

通讯作者： Yang, Xu;Zhang, Luoping

作者机构： [Wei, Chenxi; Yuan, Junlin; Yuan, Langyue; Wen, Huaxiao; Yang, Xu; Li, Hui; Wang, Kun] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Luoping; McHale, Cliona M.] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.;[Wei, Chenxi] Hunan Normal Univ, Coll Life Sci, Key Lab Ecol Safety Monitoring & Evaluat, Changsha 410081, Hunan, Peoples R China.;[Wang, Kun] NYU, Sch Med, Dept Populat Hlth, Div Biostat, New York, NY USA.

通讯机构： [Yang, Xu] C;[Zhang, Luoping] U;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.

关键词： Formaldehyde;Benzene;Hematotoxicity;Bone marrow;Myeloid progenitor

摘要： Formaldehyde (FA) is a human leukemogen and is hematotoxic in human and mouse. The biological plausibility of FA-induced leukemia is controversial because few studies have reported FA-induced bone marrow (BM) toxicity, and none have reported BM stem/progenitor cell toxicity. We sought to comprehensively examine FA hematoxicity in vivo in mouse peripheral blood, BM, spleen and myeloid progenitors. We included the leukemogen and BM toxicant, benzene (BZ), as a positive control, separately and together with FA as co-exposure occurs frequently. We exposed BALB/c mice to 3 mg/m3 FA in air for 2 weeks, mimicking occupational exposure, then measured complete blood counts, nucleated BM cell count, and myeloid progenitor colony formation. We also investigated potential mechanisms of FA toxicity, including reactive oxygen species (ROS) generation, apoptosis, and hematopoietic growth factor and receptor levels. FA exposure significantly reduced nucleated BM cells and BM-derived colony-forming unit-granulocyte–macrophage (CFU-GM) and burst-forming unit-erythroid (BFU-E); down-regulated GM-CSFRα and EPOR expression; increased ROS in nucleated BM, spleen and CFU-GM cells; and increased apoptosis in nucleated spleen and CFU-GM cells. FA and BZ each similarly altered BM mature cells and stem/progenitor counts, BM and CFU-GM ROS, and apoptosis in spleen and CFU-GM but had differential effects on other end points. Co-exposure was more potent for several end points. Thus, FA is toxic to the mouse hematopoietic system, including BM stem/progenitor cells, and it enhances BZ-induced toxic effects. Our findings suggest that FA may induce BM toxicity by affecting myeloid progenitor growth and survival through oxidative damage and reduced expression levels of GM-CSFRα and EPOR. © 2016, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： 杜俊停;李潇潇;宋静;张倩;陆林洁;...

期刊： 化学与生物工程,2017年34(9):29-34,45 ISSN：1672-5425

作者机构： 华中师范大学生命科学学院 遗传调控与整合生物学湖北省重点实验室,湖北 武汉,430079;[袁均林; 宋静; 陆林洁; 李潇潇; 张倩; 杜俊停] 华中师范大学

关键词： 三氟羧草醚;肝脏;肾脏;细胞色素c;氧化损伤

摘要： 农药三氟羧草醚是一种原卟啉原氧化酶的抑制剂。为了探究三氟羧草醚对小鼠肝脏和肾脏组织的氧化损伤,将24只雄性Balb/c小鼠随机分成4组：1个生理盐水对照组和3个剂量水平为0.1 mg·kg~（-1）、5 mg·kg~（-1）、250 mg·kg~（-1）的三氟羧草醚染毒组,14d经口灌胃染毒。制备组织匀浆液并测定组织中细胞色素c（cyt-c）、活性氧（ROS）、还原型谷胱甘肽（GSH）、脂质过氧化物丙二醛（MDA）和8-羟基脱氧鸟苷（8-OHdG）的含量。结果表明：三氟羧草醚剂量为250 mg·kg~（-1）时,肝脏和肾脏组织的脏器系数、ROS、MDA和8-OHdG的含量均有明显上升（P〈0.05）;细胞色素c和GSH的含量有显著下降（P〈0.05）;表明较高剂量（≥250 mg·kg~（-1））的三氟羧草醚能够降低肝脏和肾脏组织中细胞色素c的含量以及可造成小鼠肝脏和肾脏组织的氧化损伤。

语种： 中文

作者： 郭晴;刘志敏;李潇潇;赵云;沈世平;...

期刊： 化学与生物工程,2016年33(11):15-20,26 ISSN：1672-5425

作者机构： 华中师范大学生命科学学院 湖北省遗传调控和整合生物学重点实验室,湖北 武汉,430079;[杨旭; 袁均林; 蔡洁; 郭晴; 沈世平; 李潇潇; 李睿; 赵云; 刘志敏] 华中师范大学

关键词： 纳米二氧化钛;毒性;小鼠;皮肤暴露

摘要： 为研究纳米二氧化钛(nano-TiO2)对小鼠脑和血管的毒性,将56只小鼠随机分为8组,每组7只,分别为control、solvent control、4 mg·kg-1 nano-TiO2、20 mg·kg-1 nano-TiO2、100 mg·kg-1 nano-TiO2、500 mg·kg-1 nano-TiO2、100 mg·kg-1维生素 E(VE)+100 mg·kg-1 nano-TiO2和100 mg·kg-1 VE+500 mg·kg-1 nano-TiO2,采用皮肤涂抹的方式,对小鼠进行连续42 d 暴露。结果表明：4 mg·kg-1 nano-TiO2和20 mg·kg-1 nano-TiO2对小鼠的大脑毒性和血管损伤较低,100 mg·kg-1 nano-TiO2和500 mg·kg-1 nano-TiO2对小鼠的大脑毒性和血管损伤明显,暴露中VE 的添加对 nano-TiO2给脑和血管造成的损伤具有一定的缓解作用。初步证明 nano-TiO2皮肤暴露在一定剂量下可对小鼠脑和血管引起毒性作用,其原因可能为氧化损伤。

语种： 中文

作者： Zhao, Yun;Wei, Chenxi;Wu, Yang;Ma, Ping;Ding, Shumao;...

期刊： Toxicology Mechanisms and Methods,2016年26(2):75-81 ISSN：1537-6516

通讯作者： Yang, Xu;Shen, Dingwen

作者机构： [Yuan, Junlin; Yang, Xu; Zhao, Yun; Ding, Shumao] Cent China Normal Univ, Sect Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Peoples R China.;[Wei, Chenxi] Hunan Normal Univ, Coll Life Sci, Key Lab Ecol Safety Monitoring & Evaluat, Changsha, Hunan, Peoples R China.;[Wu, Yang; Shen, Dingwen; Ma, Ping] Hubei Univ Sci & Technol, Coll Basic Med Sci, Xianning 437100, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Shen, Dingwen] Hubei Univ Sci & technol, 88 Xianning Rd, Xianning 437100, Peoples R China.

通讯机构： [Yang, Xu] C;[Shen, Dingwen] H;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;Hubei Univ Sci & technol, 88 Xianning Rd, Xianning 437100, Peoples R China.

关键词： Formaldehyde;Hela cell;P53;Western blot;immunofluorescence;paxillin;phosphotyrosine

摘要： Formaldehyde (FA) is an environmental pollutant and an endogenous product believed to be involved in tumorigenesis. However, the underlying mechanism of observed FA effects has not been clearly defined. Paxillin is a focal adhesion protein that may play an important role in several signaling pathways. Many paxillin-interacting proteins are involved in the regulation of actin cytoskeleton organization, which is necessary for cell motility events associated with diverse biological responses, such as embryonic development, wound repair and tumor metastasis. P53 is important in multicellular organisms, where it regulates the cell cycle and thus functions as a tumor suppressor that is involved in preventing cancer. In this study, we investigated the effects of FA on paxillin-tyrosine phosphorylation and P53 expression in Hela cells by Western blot and immunofluorescence. Western blot analysis revealed that nonlethal concentrations of FA (0.5, 1.0 and 2.0 mM, with the exposure time for 0.5, 1.0 and 2.0 h, respectively) had downregulated paxillin and wild-type p53 genes expression while upregulated paxillin-tyrosine phosphorylation significantly. At the same time, phosphotyrosine at the focal adhesion sites detected by immunofluorescence assay obviously increased in Hela cells incubated with 2.0 mM FA for 2 h. The results suggested that paxillin and p53 genes expression may be involved in FA-related adverse effects and the mechanism may be involved in paxillin-tyrosine phosphorylation. © 2015 Taylor & Francis.

语种： 英文

作者： 李潇潇;尤会会;郭晴;杜俊婷;丁书茂;...

期刊： 化学与生物工程,2016年33(9):27-31 ISSN：1672-5425

作者机构： 华中师范大学生命科学学院 遗传调控与整合生物学湖北省重点实验室,湖北 武汉,430079;[袁均林; 杨旭; 郭晴; 尤会会; 李潇潇; 丁书茂; 杜俊婷] 华中师范大学

关键词： 烟嘧磺隆;人工抗原;多克隆抗体

摘要： 为了制备烟嘧磺隆的多克隆抗体、建立烟嘧磺隆残留的免疫分析方法,以2-氨基磺酰基-N ,N-二甲基烟酰胺(ASDM)和琥珀酸酐为原料合成半抗原,采用活泼酯法制备人工抗原并免疫小鼠。结果表明：经紫外可见吸收光谱扫描和聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴定,人工抗原合成成功,半抗原与卵清蛋白(OVA)、牛血清白蛋白(BSA)的偶联比分别为10.5∶1和26.9∶1。5次免疫后小鼠抗血清的效价均达到1∶16000以上,以 ASDM-BSA 为免疫原的小鼠产生了抑制烟嘧磺隆的多克隆抗体,建立的间接竞争 ELISA 方法对烟嘧磺隆的检测范围是1 ng·mL-1~10μg·mL-1。为进一步建立更为高效、灵敏和方便的烟嘧磺隆 ELISA 检测方法提供了参考。

语种： 中文

作者： Tang, Jiaqi*;Yuan, Ye;Wei, Chenxi;Liao, Xiaomei;Yuan, Junlin;...

期刊： TOXICOLOGY RESEARCH,2015年4(4):1006-1015 ISSN：2045-452X

通讯作者： Tang, Jiaqi

作者机构： [Tang, Jiaqi; Wei, Chenxi; Yuan, Junlin; Yuan, Ye; Liao, Xiaomei; Yang, Xu] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.;[Bornehag, Carl-Gustaf; Nanberg, Eewa] Karlstad Univ, Dept Hlth Sci, SE-65188 Karlstad, Sweden.;[Zhang, Yinping] Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.

通讯机构： [Tang, Jiaqi] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.

摘要： Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals. We undertook a study to ascertain the neurotoxicity of DEHP in Kunming mice. This study included three rounds of testing. In the first round, Kunming mice were exposed to different concentrations of DEHP (0, 5, 50, 500 mg kg−1 per day) after which their cognitive ability was assessed using the Morris water maze (MWM) test. The reactive oxygen species (ROS) content in tissue and the malondialdehyde (MDA) content of brains were also measured. In the second round, vitamin E (50 mg kg−1 per day) was given daily as an anti-oxidant via the intragastric route. Cognitive deficits and locomotor activity, as well as ROS and MDA contents were tested employing the same methods. In the third round, the depressive mood of mice after DEHP exposure (500 mg kg−1 per day) was measured using the open field test, the tail suspension test, and the forced swim test. The main findings of this study include: (1) a statistical association exists between DEHP oral exposure and spatial learning (DEHP 500 mg kg−1 per day) and memory (DEHP 50 mg kg−1 per day) dysfunction as ascertained by an MWM test of Kunming mice. (2) A statistical association was also found between DEHP oral exposure (50 and 500 mg kg−1 per day) and oxidative stress (ROS and MDA) of mouse brain tissue. (3) Co-administration of vitamin E (50 mg kg−1 per day) diminishes the elevation of ROS and MDA induced by DEHP (50 mg kg−1 per day) from significant levels to non-significant levels. (4) Co-administration of vitamin E (50 mg kg−1 per day) protects against mouse memory dysfunction induced by DEHP (50 mg kg−1 per day) from being significant to being not significant. (5) In the 5 mg kg−1 per day DEHP exposure groups, oxidative stress in brain tissue, and neurobehavioral changes were not found. (6) High dose DEHP exposure (500 mg kg−1 per day) may induce behavioral despair in mice. Conclusions: These data suggest that DEHP is neurotoxic with regard to cognitive ability and locomotor activity. Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals.

语种： 英文

作者： Ke, Chen-Juan;He, Ya-Hui;He, Hong-Wu;Yang, Xu;Li, Rui*;...

期刊： ANALYTICAL METHODS,2014年6(16):6381-6388 ISSN：1759-9660

通讯作者： Li, Rui

作者机构： [Yuan, Junlin; Li, Rui; Ke, Chen-Juan; Yang, Xu] Cent China Normal Univ, Sch Life Sci, Huibei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[He, Ya-Hui] Pingdingshan Ctr Bur Qual & Tech Supervis Inspect, Ping Dingshan 467000, Peoples R China.;[He, Hong-Wu] Cent China Normal Univ, Sch Chem, Inst Pesticide Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.

通讯机构： [Li, Rui] C;Cent China Normal Univ, Sch Life Sci, Huibei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.

摘要： Pyruvate dehydrogenase complex (PDHc) plays a key role in pyruvate decarboxylation, the transformation of pyruvate to acetyl-CoA. In this study, a new assay for measuring PDHc activity has been developed. 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is adopted as the electron acceptor in this method, and the change in absorbance caused by the reduction of MTT by hydroxyethyl-TPP, which is the catalyzed product of pyruvate dehydrogenase (PDH, E1), within a certain period of time is measured to reflect PDHc activity. For further impartial evaluation of the characteristics of this newly developed assay, a series of comparative studies were also conducted with several commonly used assays of PDHc activity, including the conventional spectrophotometric assay of NADH accumulation, the p-iodonitrotetrazolium violet (INT)-coupled assay, the 2,6-dichlorophenolindophenol (2,6-DCPIP) assay, and the potassium ferricyanide assay. Results have proved that the spectrophotometric assay using MTT is highly sensitive and inexpensive with little interference from the sulfhydryl compound. In conclusion, the newly established assay is applicable for activity measurement not only in purified PDH and PDH in purified PDHc, but also in crude PDH solution prepared under certain conditions; hence, the assay can indirectly reflect the activity of PDHc activity. This journal is © the Partner Organisations 2014.

语种： 英文

作者： 刘志敏;熊棣;曹凤华;梅宇飞;吴卓;...

期刊： 化学与生物工程,2014年(8):18-23 ISSN：1672-5425

作者机构： 华中师范大学生命科学学院 遗传调控与整合生物学湖北省重点实验室,湖北 武汉,430079;[袁均林; 杨旭; 梅宇飞; 吴卓; 曹凤华; 熊棣; 刘志敏] 华中师范大学

关键词： 邻苯二甲酸单丁酯;小鼠;肝;肾;氧化损伤

摘要： 为了探究邻苯二甲酸单丁酯（MBP）对小鼠肝脏和肾脏的氧化损伤，将42只 BALB/c 小鼠随机分为7组，每组6只，分别为25 mg·kg^-1、50 mg·kg^-1、100 mg·kg^-1、200 mg·kg^-1的4个 MBP 染毒组、1个100 mg·kg^-1的邻苯二甲酸二丁酯（DBP）染毒组、1个空白对照组、1个溶剂对照组。染毒期间对小鼠的体征进行观察；14 d 后取其肝脏和肾脏组织，制作小鼠肝脏和肾脏组织的切片，对肝脏和肾脏的组织学形态进行观察；制作组织匀浆液用于检测肝脏和肾脏组织细胞的 ROS、GSH、MDA 的含量，以了解 MBP 对肝脏和肾脏组织的氧化损伤作用。结果显示：各剂量组小鼠肝细胞和肾小管上皮细胞均出现不同程度的细胞核固缩、细胞水肿、空泡样变、脂肪滴增大融合等症状；ROS 和 MDA 的含量与 MBP 的染毒剂量呈正相关，GSH 的含量与 MBP 的染毒剂量呈负相关；相同剂量的 DBP 与 MBP 染毒组相比，MBP染毒组的 ROS 和 MDA 含量较高、GSH 含量较低。表明，MBP 的暴露与小鼠肝脏和肾脏组织的氧化损伤存在直接联系。

语种： 中文

作者： Ke, Y. J.;Qin, X. D.;Zhang, Y. C.;Li, H.;Li, R.;...

期刊： Human & Experimental Toxicology,2014年33(8):822-830 ISSN：0960-3271

通讯作者： Ding, S. M.

作者机构： [Qin, X. D.; Li, R.; Yang, X.; Yuan, J. L.; Ding, S. M.; Li, H.; Zhang, Y. C.; Ke, Y. J.] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Ding, S. M.] Cent China Normal Univ, Coll Life Sci, Lab Environm Biomed, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.

通讯机构： [Ding, S. M.] C;Cent China Normal Univ, Coll Life Sci, Lab Environm Biomed, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.

关键词： Formaldehyde;formaldehyde dehydrogenase;reactive oxygen species;formaldehyde content;real-time qPCR

摘要： HeLa cells were exposed to formaldehyde and its metabolic derivatives, methanol, formic acid, and acetaldehyde, to investigate that the toxicity of formaldehyde is not caused by the chemical group. After 1 h of treatment with formaldehyde, mitochondrial assays showed that low concentrations (e.g. 10 εmol/L) of formaldehyde promoted growth of the HeLa cells, while higher concentrations (e.g. ≥62.5 εmol/L) inhibited cell growth; while all four chemicals at a concentration of 125 εmol/L affected cell growth, formaldehyde affected the largest. Reactive oxygen species concentration increased with the concentration of the exposure chemical. The endogenous formaldehyde content increased the most in the formaldehyde group, but in other three groups, it did not increase as the exposure concentration increased. Expression of dehydrogenase (formaldehyde dehydrogenase (FDH)) in the formaldehyde (10.40) and methanol (10.60) groups increased significantly compared with the control (1), while it was similar to the control in formic acid (0.90) and acetaldehyde (1.10) groups. Our results suggest that formaldehyde could affect cell activity and even enter cells. Exposure to formaldehyde changes the endogenous formaldehyde concentration in cells within 24 h, and this induces expression of FDH for formaldehyde degradation to maintain the formaldehyde balance. The toxicity of formaldehyde is not caused by the carbon atoms in the aldehyde, hydroxyl, or carboxyl groups. Formaldehyde is hypothesized to be an important signaling molecule in the regulation of cell growth and maintenance of the endogenous formaldehyde level. © The Author(s) 2014.

语种： 英文

作者： Zhao, Jingyun;Yu, Xiaowei;Luo, Qing;Wei, Chenxi;Ke, Chenjuan;...

期刊： Food and Agricultural Immunology,2014年25(3):350-363 ISSN：0954-0105

通讯作者： Yang, Xu

作者机构： [Wei, Chenxi; Yuan, Junlin; Zhao, Jingyun; Luo, Qing; Ke, Chenjuan; Zuo, Haoxiao; Yang, Xu; Yu, Xiaowei] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Peoples R China.

通讯机构： [Yang, Xu] H;Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Peoples R China.

关键词： anti-BSM monoclonal antibody (anti-BSM MAb);bensulfuron methyl (BSM);food safety;indirect competitive enzyme-linked immunoassay (icELISA)

摘要： Bensulfuron methyl (BSM) is widely used for agricultural purposes and has raised health concerns, as well as ecological problems. Immunoassay would be one of the most advantaged measurements compared with traditional methods for BSM detection and measurement. In order to develop indirect competitive enzyme-linked immunoassay (icELISA), the anti-BSM monoclonal antibody (anti-BSM MAb) was produced. For the MAb production, BSM was conjugated to OVA and injected to mice with Freud's adjuvant for immunisation. Antiserum screening has revealed successful immunising. One stable hybridoma cell line (2H1) was obtained through cell fusion between spleen cells of immunised mouse and SP 2/0 cells. The MAb secreted by 2H1 cells was of high affinity and sensitivity, as well as specificity to BSM. Then the protocol of the icELISA and standard curve for BSM measurement was made and examined by controlled application. Significantly, the application has exhibited 96.530%-107.2% recovery of BSM. The produced MAb and established immunoassay may facilitate the measurement of BSM and herein help to ensure food safety and regulate environmental protection. © 2013 Taylor & Francis.

语种： 英文

作者： Ye, Ranfeng;Yu, Xiaowei;Yang, Shangyue;Yuan, Junlin;Yang, Xu*

期刊： Integrated Ferroelectrics,2013年147(1):166-174 ISSN：1058-4587

通讯作者： Yang, Xu

作者机构： [Yang, Xu] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;Huazhong Normal Univ, Coll Life Sci, Lab Environm Biomed, Wuhan 430079, Peoples R China.

通讯机构： [Yang, Xu] H;Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.

关键词： Malondialdehyde;Silica dioxide nanoparticles;Toxicity;Zebrafish embryos

摘要： To assess the aquatic ecosystem safety of silicon dioxide (SiO2) nanoparticles (NPs), we studied the toxicity of the SiO2 NPs and SiO2 bulk particles (BPs) using zebrafish. Our results show that the SiO2 NPs can significantly affect the development of zebrafish embryos, while there is no obvious effect on survival and development of embryos was found in SiO2 BPs exposure group. The content of malondialdehyde (MDA) in zebrafish larvae exposed to the SiO2 NPs for 108 hours, increased significantly in dose-dependent manner. These results suggest that the SiO2 NPs have toxic effects on the development of zebrafish embryos and the effects could be resulted by oxidative damage. Copyright © 2013 Taylor and Francis Group, LLC.

语种： 英文

作者： 尤会会;赵静云;袁烨;魏晨曦;袁均林

期刊： 环境科学学报,2013年33(4):1202-1207 ISSN：0253-2468

通讯作者： Yuan, J.

作者机构： [尤会会; 赵静云; 袁烨; 魏晨曦; 袁均林] 华中师范大学生命科学学院

通讯机构： Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, China

关键词： 邻苯二甲酸二乙基己酯;卵清蛋白;小鼠;肺功能;气道重塑

摘要： 为探讨邻苯二甲酸二乙基己酯(di-(2-ehtylhexyl)phthalate,DEHP)在单独染毒和与卵清蛋白(ovalbumin,OVA)联合染毒条件下对小鼠气道的影响及其相关机制,将Balb/c小鼠随机分为:未处理对照组(生理盐水组)、DEHP染毒组、OVA染毒组、DEHP+OVA联合染毒组(DEHP+OVA),每组雄性Balb/c小鼠6只.用OVA致敏加激发的方式制作小鼠哮喘模型.DEHP染毒组每天给予10mg·kg~(-1)DEHP灌胃,连续54d.OVA染毒组、DEHP+OVA染毒组小鼠均在第54~60d(共计1周)进行1%OVA雾化(30min·d~(-1)),每日1次,诱发哮喘.第60d进行以下操作:肺功能测试,肺泡灌洗液(bronchoalveolar lavage fluid,BALF)样品收集和肺组织中的Interleukin-17A(IL-17A)含量的测定.结果显示,与DEHP染毒组相比,DEHP与OVA联合染毒组小鼠BALF的IL-17A显著增加(p<0.05),肺功能降低(p<0.05),气道重塑,肺部细胞浸润明显.提示DEHP对小鼠哮喘模型佐剂作用的分子机制可能涉及IL-17的介导作用.

语种： 中文