摘要：
Microplastics (MP) are receiving increased attention as a harmful environmental pollutant, however information on the reproduction toxicity of MP in terrestrial animals, especially mammals, is limited. In this experiment, we investigated the impact of polystyrene microplastics (micro-PS) on the reproductive system of male mice. Healthy Balb/c mice were exposed to saline or to different doses of micro-PS for 6 weeks. The results showed that micro-PS exposure resulted in a significant decrease in the number and motility of sperm, and a significant increase in sperm deformity rate. We also detected a decrease in the activity of the sperm metabolism-related enzymes, succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), and a decrease in the serum testosterone content in the micro-PS exposure group. We found that micro-PS exposure caused oxidative stress and activated JNK and p38 MAPK. In addition, we found that when N-acetylcysteine (NAC) scavenges ROS, and when the p38 MAPK-specific inhibitor SB203580 inhibits p38MAPK, the micro-PS-induced sperm damage is alleviated and testosterone secretion improves. In conclusion, our findings suggest that micro-PS induces reproductive toxicity in mice through oxidative stress and activation of the p38 MAPK signaling pathways.
期刊：
Ecotoxicology and Environmental Safety,2019年174:75-82 ISSN：0147-6513
通讯作者：
Yuan, Junlin;Chen, Mingqing
作者机构：
[Yuan, JL; Chen, Mingqing; Yuan, Junlin; Duan, Jiufei; Deng, Ting; Xie, Xiaoman; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Yuan, JL; Chen, MQ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
DBP;DEHP;Estradiol;Hypertension;RAAS
摘要：
Epidemiological studies have shown that high molecular weight phthalates (HMW) such as diethylhexyl phthalate (DEHP), are associated with hypertension in humans, while low molecular weight phthalates (LMW) such as dibutyl phthalate (DBP), have hardly any impact on the elevation of blood pressure. However, the molecular mechanisms responsible for this difference are not completely understood. In this experiment, mice were exposed to 0.1/1/10mg/kg/day DEHP and 0.1/1/10mg/kg/day DBP for 6 weeks, and their blood pressure was monitored using the tail pressure method. The results showed that exposure to DEHP dosages of 1 or 10mg/kg/day resulted in a sharp increase in blood pressure, while exposure to DBP did not induce any significant changes in blood pressure. Investigating the renin-angiotensin-aldosterone system (RAAS) and NO pathway in mice exposed to DEHP, we found that levels of angiotensin-converting enzyme (ACE) and angiotensin II (AngII) increased with increasing exposure to DEHP, and the expression of nitric oxide synthase (eNOS) and the level of NO decreased. Treatment with ACE inhibitor (ACEI) to block the ACE pathway inhibited the enhancement of RAAS expression, inhibited the increase in blood pressure, and inhibited the decrease in NO levels induced by DEHP. However, the expression of ACE, AngII, AT1R, and eNOS in the DBP treatment groups showed no significant changes. When examining estradiol in vivo, we found that exposure to DBP resulted in a significant increase in the level of estradiol, while exposure to DEHP did not lead to a significant change. When ICI182780 was used to block the estradiol receptors, any increase in the level of NO induced by DBP exposure, was inhibited. These results indicate that exposure to DEHP induces an increase in mouse blood pressure through RAAS, and the different effects of DEHP and DBP on blood pressure are partly due to the different estradiol levels induced by DEHP and DBP.
作者：
Li Xiao-Xiao;Ding Shu-Mao;Yang Xu;Yuan Jun-Lin*
期刊：
分析化学,2018年46(1):27-32 ISSN：0253-3820
通讯作者：
Yuan Jun-Lin
作者机构：
[Ding Shu-Mao; Yuan Jun-Lin; Yang Xu; Li Xiao-Xiao] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Yuan Jun-Lin] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
期刊：
Toxicology and Applied Pharmacology,2017年324(2):36-44 ISSN：0041-008X
通讯作者：
Xiang, Shuanglin;Yang, Xu
作者机构：
[Chen, Mouying; Wei, Chenxi; Qiu, Feng; Xiang, Shuanglin] Hunan Normal Univ, Sch Life Sci, Key Lab Ecol Safety Monitoring & Evaluat, Changsha 410081, Hunan, Peoples R China.;[Wei, Chenxi; Yuan, Junlin; You, Huihui; Wen, Huaxiao; Yang, Xu] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan 430079, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Sch Life Sci, 152 Luo Yu Rd, Wuhan 430079, Hubei, Peoples R China.;[Xiang, Shuanglin] Hunan Normal Univ, Sch Life Sci, 36 Lu Shan Rd, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Xiang, Shuanglin] H;[Yang, Xu] C;Hunan Normal Univ, Sch Life Sci, 36 Lu Shan Rd, Changsha 410081, Hunan, Peoples R China.;Cent China Normal Univ, Sch Life Sci, 152 Luo Yu Rd, Wuhan 430079, Hubei, Peoples R China.
摘要：
Formaldehyde (FA) is a human leukemogen. Since there is a latency period between initial FA exposure and the development of leukemia, the subsequent impact of FA on hematopoietic stem or progenitor cells (HSCs/HPCs) in post-exposure stage is crucial for a deep understanding of FA-induced hematotoxicity. BALB/c mice were exposed to 3 mg/m(3) FA for 2 weeks, mimicking occupational exposure, and were monitored for another 7 days post-exposure. Meanwhile, we included benzene (BZ) as a positive control, separately and together with FA because co-exposure occurs frequently. After 7-day recovery, colonies of progenitors for CFU-GM and BFU-E, and nucleated bone marrow cells in FA-exposed mice were comparable to controls, although they were significantly reduced during exposure. Levels of reactive oxygen species (ROS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in CFU-GM and BFU-E from FA-exposed mice were higher than controls, although the increase in 8-OHdG was not significant. Granulocyte-macrophage colony stimulating factor (GM-CSF) level in the FA group was lower than controls, but the expression level for the receptor was not upregulated. It suggests that HSCs/HPCs in FA-exposed mice respond to a small amount of GM-CSF and proliferate rapidly, which may cause a possible risk of expansion of abnormal stem/progenitor cell clones. FA co-exposure with BZ was more potent for promoting CFU-GM formation and inducing ROS in BFU-E and 8-OHdG in CFU-GM during the post-exposure period. The compensation of myeloid progenitors with elevated ROS and 8-OHdG may lead to a risk of transforming normal HSCs/HPCs to leukemic stem/progenitor cells. Thus, co-exposure may pose a greater leukemia risk. (C) 2017 Elsevier Inc. All rights reserved.
通讯机构：
[Tang, Jiaqi] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.
摘要：
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals. We undertook a study to ascertain the neurotoxicity of DEHP in Kunming mice. This study included three rounds of testing. In the first round, Kunming mice were exposed to different concentrations of DEHP (0, 5, 50, 500 mg kg−1 per day) after which their cognitive ability was assessed using the Morris water maze (MWM) test. The reactive oxygen species (ROS) content in tissue and the malondialdehyde (MDA) content of brains were also measured. In the second round, vitamin E (50 mg kg−1 per day) was given daily as an anti-oxidant via the intragastric route. Cognitive deficits and locomotor activity, as well as ROS and MDA contents were tested employing the same methods. In the third round, the depressive mood of mice after DEHP exposure (500 mg kg−1 per day) was measured using the open field test, the tail suspension test, and the forced swim test. The main findings of this study include: (1) a statistical association exists between DEHP oral exposure and spatial learning (DEHP 500 mg kg−1 per day) and memory (DEHP 50 mg kg−1 per day) dysfunction as ascertained by an MWM test of Kunming mice. (2) A statistical association was also found between DEHP oral exposure (50 and 500 mg kg−1 per day) and oxidative stress (ROS and MDA) of mouse brain tissue. (3) Co-administration of vitamin E (50 mg kg−1 per day) diminishes the elevation of ROS and MDA induced by DEHP (50 mg kg−1 per day) from significant levels to non-significant levels. (4) Co-administration of vitamin E (50 mg kg−1 per day) protects against mouse memory dysfunction induced by DEHP (50 mg kg−1 per day) from being significant to being not significant. (5) In the 5 mg kg−1 per day DEHP exposure groups, oxidative stress in brain tissue, and neurobehavioral changes were not found. (6) High dose DEHP exposure (500 mg kg−1 per day) may induce behavioral despair in mice. Conclusions: These data suggest that DEHP is neurotoxic with regard to cognitive ability and locomotor activity.
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals.
作者：
Ke, Y. J.;Qin, X. D.;Zhang, Y. C.;Li, H.;Li, R.;...
期刊：
Human & Experimental Toxicology,2014年33(8):822-830 ISSN：0960-3271
通讯作者：
Ding, S. M.
作者机构：
[Qin, X. D.; Li, R.; Yang, X.; Yuan, J. L.; Ding, S. M.; Li, H.; Zhang, Y. C.; Ke, Y. J.] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Ding, S. M.] Cent China Normal Univ, Coll Life Sci, Lab Environm Biomed, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Ding, S. M.] C;Cent China Normal Univ, Coll Life Sci, Lab Environm Biomed, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.
摘要：
Bensulfuron methyl (BSM) is widely used for agricultural purposes and has raised health concerns, as well as ecological problems. Immunoassay would be one of the most advantaged measurements compared with traditional methods for BSM detection and measurement. In order to develop indirect competitive enzyme-linked immunoassay (icELISA), the anti-BSM monoclonal antibody (anti-BSM MAb) was produced. For the MAb production, BSM was conjugated to OVA and injected to mice with Freud's adjuvant for immunisation. Antiserum screening has revealed successful immunising. One stable hybridoma cell line (2H1) was obtained through cell fusion between spleen cells of immunised mouse and SP 2/0 cells. The MAb secreted by 2H1 cells was of high affinity and sensitivity, as well as specificity to BSM. Then the protocol of the icELISA and standard curve for BSM measurement was made and examined by controlled application. Significantly, the application has exhibited 96.530%–107.2% recovery of BSM. The produced MAb and established immunoassay may facilitate the measurement of BSM and herein help to ensure food safety and regulate environmental protection.
