作者机构：
[Rao, Yan; Huang, Xiaoxiao] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.;[Yang, Xu; Ma, Ping] Hubei Univ Sci & Technol, Xianning, Peoples R China.;[Zhao, Wei; Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Yang, Xu] H;[Yang, Xu] C;Hubei Univ Sci & Technol, Xianning, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 8th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN) / Symposium of Materials Science and Technology (SOMST)
会议时间：
OCT 13-16, 2017
会议地点：
Tianjin, PEOPLES R CHINA
会议主办单位：
[Huang, Xiaoxiao;Rao, Yan] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.^[Ma, Ping;Yang, Xu] Hubei Univ Sci & Technol, Xianning, Peoples R China.^[Zhao, Wei;Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
摘要：
Jasmonate ZIM-domain (JAZ) proteins and DELLA proteins are key negative regulators of jasmonates (JAs) and gibberellin (GA) signaling, respectively. In this study, we found JA and GA synergistically promote fiber cell initiation. We characterized the cellular function of a JAZ protein (GhJAZ3), and a DELLA protein (GhSLR1) of cotton (Gossypium hirsutum). GhJAZ3 is specifically expressed in elongating fibers, while GhSLR1 is expressed in different tissues and at a relatively higher level in 3 DPA ovules. GhSLR1 and GhJAZ3 proteins are localized in the cell nucleus. Yeast two-hybrid analysis indicated that GhSLR1, GhJAZ3 and GhDEL65 could interact with each other, and GhSLR1 could also interact with GhBZR1. Overexpression of GhJAZ3 in Arabidopsis increased hypocotyl and root length, leaf trichome length, and plant height, but decreased the number of leaf trichome, while overexpression of GhSLR1 in Arabidopsis decreased hypocotyl length, leaf trichome length and density. Expression of several leaf trichome initiation determinators (GL3, GL2, TTG2 and MYB23) was down-regulated in GhJAZ3 or GhSLR1 transgenic Arabidopsis, while expression of the cell elongation related genes (EXP1, EXP8, EXPL2 and XTH4) was altered in the GhJAZ3 and GhSLR1 transgenic Arabidopsis. Taken together, these results demonstrate that GhJAZ3 and GhSLR1 function in jasmonate and gibberellin mediated epidermal cell differentiation and elongation.
作者机构：
[Huang, Xiaoxiao] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.;[Zhao, Wei; Yang, Xu; Peng, Mei] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Zhao, Xiaoyan] Hubei Univ Med, Clin Coll 3, Shiyan, Hubei, Peoples R China.
通讯机构：
[Yang, Xu] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 8th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN) / Symposium of Materials Science and Technology (SOMST)
会议时间：
OCT 13-16, 2017
会议地点：
Tianjin, PEOPLES R CHINA
会议主办单位：
[Huang, Xiaoxiao] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.^[Peng, Mei;Zhao, Wei;Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.^[Zhao, Xiaoyan] Hubei Univ Med, Clin Coll 3, Shiyan, Hubei, Peoples R China.
关键词：
SWCNTs;nerve cells;protection effect;viability;vitamin C
作者机构：
[李森; 董庆利; 陈国薇; 吴嫚; 丁承超; 刘武康; 刘箐] School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China;[罗勤] College of Life Sciences, Central China Normal University, Wuhan, 430079, China
作者机构：
[卢美玲; 李睿; 赵云; 朱雨晴; Ge, Jing; 江清英; 杨旭; 郭晴] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
期刊：
Journal of Biological Chemistry,2017年292(7):2933-2943 ISSN：0021-9258
通讯作者：
Wu, Kongming;Liu, Kaiyu
作者机构：
[Hu, Ruqin; Xiao, Yutao; Wu, Kongming; Liang, Gemei] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, West Yuanmingyuan Rd, Beijing 100193, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Hu, Ruqin; Liu, Kaiyu; Yang, Yongbo; Dai, Qing] Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.;[Soberon, Mario; Pacheco, Sabino; Bravo, Alejandra] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
通讯机构：
[Wu, Kongming; Liu, Kaiyu] C;Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, West Yuanmingyuan Rd, Beijing 100193, Peoples R China.;Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.
摘要：
Transgenic plants that produce Bacillus thuringiensis (Bt) crystalline (Cry) toxins are cultivated worldwide to control insect pests. Resistance to B. thuringiensis toxins threatens this technology, and although different resistance mechanisms have been identified, some have not been completely elucidated. To gain new insights into these mechanisms, we performed multiple back-crossing from a 3000-fold Cry1Ac-resistant BtR strain from cotton bollworm (Helicoverpa armigera), isolating a 516-fold Cry1Ac-resistant strain (96CAD). Cry1Ac resistance in 96CAD was tightly linked to a mutant cadherin allele (mHaCad) that contained 35 amino acid substitutions compared with HaCad from a susceptible strain (96S). We observed significantly reduced levels of the mHaCad protein on the surface of the midgut epithelium in 96CAD as compared with 96S. Expression of both cadherin alleles from 96CAD and 96S in insect cells and immunofluorescence localization in insect midgut tissue sections showed that the HaCAD protein from 96S localizes on the cell membrane, whereas the mutant 96CAD-mHaCad was retained in the endoplasmic reticulum (ER). Mapping of the mutations identified a D172G substitution mainly responsible for cadherin mislocalization. Our finding of a mutation affecting membrane receptor trafficking represents an unusual and previously unrecognized B. thuringiensis resistance mechanism.
作者机构：
[Wu, Yang; Li, Yan; Wang, Peng-Cheng; Gong, Zhi-Yong] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Pi, Qing-Meng] MIT, Harvard MIT Div Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA.;[Li, Yan] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Pi, Qing-Meng] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthoped Surg, Shanghai 200233, Peoples R China.
通讯机构：
[Wu, Yang] W;[Yang, Xu] C;Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Exposure to ambient fine particulate matter (FPM) has been thought to be associated with cardiovascular disease. However, the pathogenesis remains largely unknown. Animal models have been widely used in toxicological research, but species difference makes it impossible to directly translate discoveries from animals to humans. In this study, we developed a 3D functional human microvascular network in a microfluidic device. The established model enables endothelial cells to form vessel-like microtissues and have physiological functions which are closer to cells in human blood vessels. The perfusable microvasculature allows the delivery of nutrients, and oxygen, as well as flow-induced mechanical stimuli into the luminal space of the endothelium. The microflow effectively mimic the blood flow in human vessels. FPMs were introduced into this physiologically human vessel-like microenvironment following the fluid flow. The vascular toxicity was evaluated based on this organotypic 3D microvessel model. Our results demonstrated that intravascular accumulation of FPM could enhance ROS generation which may further cause endothelial dysfunction by oxidative stress. This is expressed in disorder of NO expression and IL-6 up-regulation. These are expected to enhance endothelial inflammation which might in turn accelerate coagulation that is associated with thrombosis. Human organotypic 3D microvessel models provide a possible bridge for how the research outcomes translate to humans. These models could partly simulate the physiological responses of human vessels to FPM stimulation. This simple and versatile platform can be used for a wide range of applications in vascular physiology studies of particulate matter in the context of cardiovascular disease.
作者：
Wang, Jia-Lin;Saha, Tusar T.;Zhang, Yang;Zhang, Changyu;Raikhel, Alexander S.*
期刊：
Journal of Biological Chemistry,2017年292(24):10306-10315 ISSN：0021-9258
通讯作者：
Raikhel, Alexander S.
作者机构：
[Saha, Tusar T.; Zhang, Changyu; Raikhel, Alexander S.; Zhang, Yang; Wang, Jia-Lin] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA.;[Wang, Jia-Lin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Saha, Tusar T.; Raikhel, Alexander S.] Univ Calif Riverside, Inst Integrat Genom Biol, Riverside, CA 92521 USA.;[Zhang, Yang] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.;[Zhang, Changyu] Guizhou Univ, Coll Agr, Guizhou Key Lab Plant Pest Management Mt Reg, Guiyang 550025, Peoples R China.
通讯机构：
[Raikhel, Alexander S.] U;Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA.;Univ Calif Riverside, Inst Integrat Genom Biol, Riverside, CA 92521 USA.
摘要：
Juvenile hormone (JH) controls many biological activities in insects, including development, metamorphosis, and reproduction. In the Aedes aegypti mosquito, a vector of dengue, yellow fever, chikungunya, and zika viruses, the metabolic tissue (the fat body, which is an analogue of the vertebrate liver) produces yolk proteins for developing oocytes. JH is important for the fat body to acquire competence for yolk protein production. However, the molecular mechanisms of how JH promotes mosquito reproduction are not completely understood. In this study we show that stimulation of the JH receptor methoprene-tolerant (Met) activates expression of genes encoding the regulator of ribosome synthesis 1 (RRS1) and six ribosomal proteins (two ribosomal large subunit proteins, two ribosomal small subunit proteins, and two mitochondrial ribosomal proteins). Moreover, RNAi-mediated depletion of RRS1 decreased biosynthesis of the ribosomal protein L32 (RpL32). Depletion of Met, RRS1, or RpL32 led to retardation of ovarian growth and reduced mosquito fecundity, which may at least in part have resulted from decreased vitellogenin protein production in the fat body. In summary, our results indicate that JH is critical for inducing the expression of ribosomal protein genes and demonstrate that RRS1 mediates the JH signal to enhance both ribosomal biogenesis and vitellogenesis.
作者机构：
[安结然; 陆林洁; 蔡洁; 杜俊停; 丁书茂] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, 430079, China
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China
