Validation of an in vivo electrochemical immunosensing platform for simultaneous detection of multiple cytokines in Parkinson’s disease mice model
作者：
Shen, Zhuping;Huang, Jiawei;Wei, Hui;Niu, Huan;Li, Bitong;...
期刊：
Bioelectrochemistry ,2020年134:107532 ISSN：1567-5394
通讯作者：
Liu, Guozhen
作者机构：
[Shen, Zhuping; Liu, Guozhen; Wei, Hui] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Wuhan 430079, Peoples R China.;[Li, Rui; Huang, Jiawei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Niu, Huan; Li, Bitong; Liu, Guozhen] Univ New South Wales, Fac Engn, Grad Sch Biomed Engn, Sydney, NSW 2052, Australia.;[Liu, Guozhen] Univ New South Wales, Australian Ctr NanoMed, Sydney, NSW 2052, Australia.;[Liu, Guozhen] Univ New South Wales, Grad Sch Biomed Engn, Sydney, NSW, Australia.
通讯机构：
[Liu, Guozhen] U;Univ New South Wales, Grad Sch Biomed Engn, Sydney, NSW, Australia.
关键词：
Cytokines;Electrochemical immunosensors;Multiplex sensing;In vivo detection;Parkinson's disease;Mouse brain
摘要：
Parkinson's Disease (PD) is a neurodegenerative chronic disorder which destroys brain tissue and result in impaired movement. Early diagnosis of PD before the appearance of clinical symptom is vital for effective treatment. High levels of proinflammatory cytokines found in PD patient's brains, as natural inflammation response product, are potential biomarkers for PD detection in the early stage. Herein, we developed an in vivo electrochemical immunosensing device based on glassy carbon rod to simultaneously detect three proinflammatory cytokines (IL-1β, IL-6 and TNF-α). The levels of IL-1β, IL-6 and TNF-α secreted by N2a cells significantly increased within 24 h after lipopolysaccharide (LPS) stimulation. Under in vivo conditions, the concentrations of IL-1β, IL-6 and TNF-α in PD model group achieved by injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intraperitoneally, were significantly higher than those in the control mouse group. The concentrations of three cytokines in vivo/vitro detected by this immunosensing device was comparable to that obtained by ELISA. Furthermore, this deployable immunosensing device was proved to be highly sensitive with the limits of detection (LODs) of 5 pg mL−1 for each cytokine, specific and reliable, suggesting its potential to be a universal immunosensing platform for early identification and diagnosis of PD in vivo in the future. © 2020 Elsevier B.V.
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英文
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Combined exposure to formaldehyde and PM2.5: Hematopoietic toxicity and molecular mechanism in mice
作者：
Ge, Jing;Yang, Honglian;Lu, Xianxian;Wang, Shenqi;Zhao, Yun;...
期刊：
Environment International ,2020年144:106050 ISSN：0160-4120
通讯作者：
Li, Rui;Zhang, Luoping
作者机构：
[Li, Rui; Zhao, Yun; Huang, Jiawei; Lu, Xianxian; Ge, Jing] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, Luoping] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.;[Xi, Zhuge; Yang, Honglian] Tianjin Inst Environm & Operat Med, Tianjin 300050, Peoples R China.;[Wang, Shenqi; Ge, Jing] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.
通讯机构：
[Li, Rui] C;[Zhang, Luoping] U;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.
关键词：
PM2.5;Formaldehyde;Combined exposure;Hematopoietic toxicity;Molecular mechanism
摘要：
PM2.5 and formaldehyde (FA) are major outdoor and indoor air pollutants in China, respectively, and both are known to be harmful to human health and to be carcinogenic. Of all the known chronic health effects, leukaemia is one of the most serious health risks associated with these two pollutants. To explore the influence and underlying mechanisms of exposure to formaldehyde and PM2.5 on hematopoietic toxicity, we systematically studied the toxicity induced in hematopoietic organs: bone marrow (BM); spleen; and myeloid progenitor cells (MPCs). Male Balb/c mice were exposed to: PM2.5 (20, 160 μg/kg·d) at a dose of 40 μL per mouse or formaldehyde (0.5, 3.0 mg/m3) for 8 h per day for 2 weeks or co-exposed to formaldehyde and PM2.5 (20 μg/kg·d PM2.5 + 0.5 mg/m3 FA, 20 μg/kg·d PM2.5 + 3 mg/m3 FA, 160 μg/kg·d PM2.5 + 0.5 mg/m3 FA, 160 μg/kg·d PM2.5 + 3 mg/m3 FA) for 2 weeks. Similar toxic effects were found in the formaldehyde-only and PM2.5-only groups, including significant decrease of blood cells and MPCs, along with decreased expression of hematopoietic growth factors. In addition, individual exposure of formaldehyde or PM2.5 increased oxidative stress, DNA damage and immune system disorder by destroying the balance of Th1/Th2, and Treg/Th17. DNA repair was markedly inhibited by deregulating the mammalian target of rapamycin (mTOR) pathway. Combined exposure to PM2.5 and formaldehyde led to more severe effects. Administration of Vitamin E (VE) was shown to attenuate these effects. In conclusion, our findings suggested that PM2.5 and formaldehyde may induce hematopoietic toxicity by reducing the expression of hematopoietic growth factors, increasing oxidative stress and DNA damage, activating the ‘immune imbalance’ pathway and suppressing the DNA-repair related mTOR pathway. The hematopoietic toxicity induced by combined exposure of PM2.5 and formaldehyde might provide further insights into the increased incidence of hematological diseases, including human myeloid leukaemia. © 2020 The Authors
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英文
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Long Term Aquatic Vegetation Dynamics in Longgan Lake Using Landsat Time Series and Their Responses to Water Level Fluctuation
作者：
Tan, Wenxia;Xing, Jindi;Yang, Shao* ;Yu, Gongliang;Sun, Panpan;...
期刊：
Water ,2020年12(8) ISSN：2073-4441
通讯作者：
Yang, Shao
作者机构：
[Xing, Jindi; Jiang, Yan; Tan, Wenxia] Cent China Normal Univ, Coll Urban & Environm Sci, Key Lab Geog Proc Anal & Simulat Hubei Prov, Wuhan 430079, Peoples R China.;[Yang, Shao; Sun, Panpan] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Yu, Gongliang] Wuhan Univ, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
通讯机构：
[Yang, Shao] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
关键词：
spatial-temporal dynamics;aquatic vegetation;water level fluctuation;Longgan lake;Google Earth Engine
摘要：
Aquatic vegetation in shallow freshwater lakes are severely degraded worldwide, even though they are essential for inland ecosystem services. Detailed information about the long term variability of aquatic plants can help investigate the potential driving mechanisms and help mitigate the degradation. In this paper, based on Google Earth Engine cloud-computing platform, we made use of a 33-year (1987-2019) retrospective archive of moderate resolution Landsat TM, ETM + and OLI satellite images to estimate the extent changes in aquatic vegetation in Longgan Lake from Middle Yangtze River Basin in China using the modified enhanced vegetation index, including emerged, floating-leaved and floating macrophytes. The analysis of the long term dynamics of aquatic vegetation showed that aquatic vegetation were mainly distributed in the western part of the lake, where lake bottom elevation ranged from 11 to 12 m, with average water depth of less than 1 m in spring. The vegetation area variation for the 33-year period were divided into six stages. In years with heavy precipitation, the vegetation area decreased sharply. In the following years, the area normally restored. Aquatic vegetation area had a significant negative correlation with the spring water level and summer water level. The results showed that aquatic vegetation was negatively affected when water depth exceeded 2.5 m in May and 5 m in summer. It is recommended that water depth remain close to 1 m in spring and close to 3 m in summer for aquatic vegetation growth. Our study provide quantitative evidence that water-level fluctuations drive vegetation changes in Longgan Lake, and present a basis for sustainable lake restoration and management. © 2020 by the authors.
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英文
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Study of the neurotoxicity of indoor airborne nanoparticles based on a 3D human blood-brain barrier chip
作者：
Li, Yan;Liu, Yan;Hu, Chuanlin;Chang, Qing;Deng, Qihong;...
期刊：
Environment International ,2020年143:105598 ISSN：0160-4120
通讯作者：
Yang, Xu;Wu, Yang
作者机构：
[Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Chinese Minist Educ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Hu, Chuanlin] Wuhan Univ Technol, State Key Lab Silicate Mat Architectures, Wuhan 430070, Peoples R China.;[Yang, Xu; Deng, Qihong] Cent South Univ, XiangYa Sch Publ Hlth, Changsha 410083, Peoples R China.;[Chang, Qing] Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Kowloon, Clear Water Bay, Hong Kong, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Xu] C;[Wu, Yang] W;Cent China Normal Univ, Sch Life Sci, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Peoples R China.
关键词：
Indoor nanoscale particulate matter;3D microfluidic chip;blood-brain barrier;Oxidative stress;Nrf2/Keap1 signal pathway
摘要：
Background: There is growing public awareness regarding the health effects of indoor nanoscale particulate matter (INPM) since people spend the majority of their time indoors. INPM could have a direct entry route into the brain via the axons of the olfactory nerve and migrating across the blood-brain barrier (BBB). Using animals to explore this possibility is not a reliable method to fully demonstrate human physiological responses. We, therefore, set out to develop a human 3D functional blood-brain barrier model to examine the potential effects of INPM on the cerebral nervous system. Methods: Human astrocytes were co-cultured and human umbilical vein endothelial cells in 3D within a microfluidic chip to simulate the micro-complex physiological structure of the human BBB. This 3D human organotypic model has then been made to investigate any INPM-induced BBB dysfunction linked to potential cellular responses. Results: A 3D human functional blood-brain barrier was constructed in this study. We observed the translocation of INPM across the blood-brain barrier. The 3D human organotypic chip initially reflected damage to the nervous system with abnormal astrocyte proliferation and a decline in cell viability. We also looked at the behavior of oxidative stress-related biomarkers (ROS, GSH-Px, and MDA). INPM was implicated in aggravating inflammation via reactive oxygen species (ROS). The Keap1-Nrf2-ARE pathway is a key mechanism in cellular resistance to oxidative stress by mediating and activating a variety of antioxidant and detoxification enzymes. Following ROS accumulation, INPM induced abnormal expression of nuclear transcription factor Nrf2. This behavior disturbed the expression of, γ-glutamate synthase (γ-GCS) and heme oxygenase (HO-1), which further exacerbated the imbalance of the antioxidant system. Conclusions: This functional 3D human organotypic chip effectively mimics the physiological response of the human BBB. The chip provides a micro-complex structure to simulate the internal environment of the human blood-brain barrier, and partially simulates the physiological responses of the BBB to INPM exposure. Based on this model, INPM was shown to affect the blood-brain barrier biofunction by disrupting the Keap1-Nrf2-ARE pathways. © 2020 The Authors
语种：
英文
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Study on visual positioning and evaluation of automatic evisceration system of chicken
作者：
Chen, Yan* ;Wang, Shucai;Bi, Jie;Ai, Hui*
期刊：
Food and Bioproducts Processing ,2020年124:222-232 ISSN：0960-3085
通讯作者：
Chen, Yan;Ai, Hui
作者机构：
[Chen, Yan] Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430048, Peoples R China.;[Chen, Yan] Wuhan Donghu Univ, Engn Coll, Wuhan 430212, Peoples R China.;[Wang, Shucai] Huazhong Agr Univ, Coll Engn, Wuhan 430079, Peoples R China.;[Bi, Jie] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430048, Peoples R China.;[Ai, Hui] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Chen, Yan] W;[Ai, Hui] C;Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430048, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
关键词：
Breakage rate;Chicken;Evisceration;Machine vision;Residual rate
摘要：
Automatic evisceration is a very important production technology in the chicken industry. In this study, an automatic chicken eviscerating robot system was developed and evaluated based on machine vision for locating the chicken carcasses and anal incision. This system consisted of chicken carcass conveying device, robotic manipulator, end-effector, control system and machine vision system. Based on machine vision, this system employed color space conversion, threshold segmentation, morphological operation method and active contour algorithm to obtain the chicken carcass images of the removed wings and legs. Subsequently, anal incision contour was extracted and its position was detected by the maximum inscribed circle algorithm. All the algorithms were implemented in the system for recognizing the individual carcass and automatically locating the targets of different sizes, which guided the manipulator to complete the evisceration. Present results indicated that the maximum location deviation of contour centroid of chicken carcass and anal incision was 5 and 4 pixels, respectively, while the average time of image processing was 954 ms. Then the eviscerating manipulator could accurately grasp the viscera, the residual rate and the breakage rate of viscera were 6.2% and 15%, respectively. The high accuracy obtained from the evaluation results shows that the machine vision system can be applied successfully to robotic evisceration in the processing of chicken. Hence, the development of the method for the eviscerating robot is necessary and significant, in particular for providing great foundation for robotic evisceration in poultry industry. © 2020 Institution of Chemical Engineers
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英文
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Functional human 3D microvascular networks on a chip to study the cytocompatibility of alpha-MnO2 nanowire
作者：
Li, Yan;Wang, Pengcheng;Gong, Zhiyong;Wu, Yang* ;Yang, Xu*
期刊：
Ferroelectrics ,2019年546(1):13-24 ISSN：0015-0193
通讯作者：
Wu, Yang;Yang, Xu
作者机构：
[Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Wu, Yang; Gong, Zhiyong] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wu, Yang] W;[Yang, Xu] C;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 9th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN)
会议时间：
AUG 17-20, 2018
会议地点：
Hohhot, PEOPLES R CHINA
会议主办单位：
[Li, Yan;Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Gong, Zhiyong;Wu, Yang] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
关键词：
3D cell cuture;MnO2 nanowires;oxidative stress;vascular toxicity
摘要：
A 3D functional human microvascular network in a microfluidic device was developed in this study. The established model enables endotheilal cells to form vessel-like microtissues and behave physiological functions which are closer to cells in human blood vessels. It provides a possible bridge for how the research outcomes translate to humans. The ɑ-MnO2 nanowires, which were successfully synthesized using the hydrothermal approach. The vascular toxicity was evaluated based on this organotypic 3D microvessel model. Our results demonstrated that ɑ-MnO2 nanowires can induce cytotoxicity in dose-dependently. Because the accumulation of reactive oxygen species (ROS) and depletion of glutathione (GSH) are observed in the nanowire-treated cells. In addition, intravascular accumulation of ɑ-MnO2 nanowires could induce endothelial dysfunction by oxidative stress. This is expressed in disorder of NO expression and IL-6 up-regulation. Based on these results, we concluded that a mechanism for ɑ-MnO2 nanowire-induced vascular toxicity, which involves the accumulation of ROS, up-regulation of oxidative stress and inflammatory factors. This investigation may provide a fundamental insight to understand the nanotoxicity of wire-shaped nanomaterials. © 2019, © 2019 Taylor & Francis Group, LLC.
语种：
英文
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Role of transient receptor potential cation channel subfamily V member 1 (TRPV1) on ozone-exacerbated allergic asthma in mice
作者：
Li, Jinquan;Chen, Yushan;Chen, Qiao Yi;Liu, Dan;Xu, Lang;...
期刊：
Environmental Pollution ,2019年247(Apr.):586-594 ISSN：0269-7491
通讯作者：
Zeng, Yan;Guo, Zhenzhong
作者机构：
[Cheng, Guirong; Xu, Lang; Li, Jinquan; Chen, Yushan; Zeng, Yan; Liu, Dan] Wuhan Univ Sci & Technol, Sch Med, Brain & Cognit Dysfunct Res Ctr, Wuhan 430065, Hubei, Peoples R China.;[Li, Jinquan; Guo, Zhenzhong] Wuhan Univ Sci & Technol, Med Coll, Hubei Prov Key Lab Occupat Hazard Identificat & C, Wuhan 430065, Hubei, Peoples R China.;[Li, Jinquan; Zeng, Yan] Wuhan Univ Sci & Technol, Big Data Sci & Engn Res Inst, Wuhan 430065, Hubei, Peoples R China.;[Chen, Qiao Yi] NYU, Dept Environm Med, Sch Med, 57 Old Forge, Tuxedo Pk, NY 10987 USA.;[Yang, Xu] Cent China Normal Univ, Sect Environm Biomed, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Zeng, Yan; Guo, Zhenzhong] W;Wuhan Univ Sci & Technol, Sch Med, Brain & Cognit Dysfunct Res Ctr, Wuhan 430065, Hubei, Peoples R China.;Wuhan Univ Sci & Technol, Med Coll, Hubei Prov Key Lab Occupat Hazard Identificat & C, Wuhan 430065, Hubei, Peoples R China.
关键词：
Allergic asthma;Calcitonin gene-related peptide;Ozone;Thymic stromal lymphopoietin;Transient receptor potential cation channel subfamily V member1
摘要：
Around the globe, worsening air pollution is spawning major public health and environmental concerns, especially in the poorest and most populous cities. As a major secondary air pollutant, ozone is a potential risk factor for exacerbated asthma, although the underlying mechanisms remain uncertain. In this study, we aim to investigate the role of ozone on asthma exacerbation using a classic asthmatic model with allergic airway inflammation by treating Balb/c mice with ovalbumin (OVA). Our study shows ozone exposure significantly exacerbated OVA-induced asthmatic phenotypes, including serum immunoglobulin, Th cytokines, inflammatory cell counts, mucus production, airway remodeling, and airway hyper responsiveness (AHR). Interestingly, expression of transient receptor potential cation channel subfamily V memberl (TRPV1) was also significantly elevated in ozone-exacerbated asthmatic mice and that treatment with TRPVI antagonist effectively suppressed AHR, airway inflammation and remodeling. The underlying mechanisms of these effects may be associated with suppression of neuropeptide calcitonin gene-related peptide (CGRP) and thymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine. Base on the role of TRPV1 in allergic asthma, this study further revealed that inhibition of TRPVI by TRPV1 antagonist has significant anti-inflammatory effects on ozone-induced asthma exacerbation in this study. Induction of TRPVI expression may be an important mechanism underlying the increased risks for asthma after exposure to environmental pollutants. (C) 2019 Elsevier Ltd. All rights reserved.
语种：
英文
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Liquid biopsy of circulating tumor DNA and biosensor applications
作者：
Li, Xuanying;Ye, Mengsha;Zhang, Weiying;Tan, Duo;Jaffrezic-Renault, Nicole;...
期刊：
Biosensors and Bioelectronics ,2019年126:596-607 ISSN：0956-5663
通讯作者：
Guo, Zhenzhong
作者机构：
[Li, Xuanying; Tan, Duo; Ye, Mengsha; Guo, Zhenzhong] Wuhan Univ Sci & Technol, Coll Med, Sch Publ Hlth, Hubei Prov Key Lab Occupat Hazard Identificat & C, Wuhan 430065, Hubei, Peoples R China.;[Zhang, Weiying] Jianghan Univ, Inst Interdisciplinary Res, Key Lab Optoelect Chem Mat & Devices, Minist Educ, Wuhan 430056, Hubei, Peoples R China.;[Jaffrezic-Renault, Nicole] Univ Lyon, UMR CNRS 5280, Inst Analyt Sci, 5 La Doua St, F-69100 Villeurbanne, France.;[Yang, Xu] Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Guo, Zhenzhong] W;Wuhan Univ Sci & Technol, Coll Med, Sch Publ Hlth, Hubei Prov Key Lab Occupat Hazard Identificat & C, Wuhan 430065, Hubei, Peoples R China.
关键词：
Bioreceptor;Biosensor;Biotransducer;Circulating tumor DNA;Liquid biopsy;Signal amplification strategies
摘要：
Circulating tumor DNA (ctDNA) as a class of liquid biopsy is a type of gene fragment that contains tumor-specific gene changes in body fluids such as human peripheral blood. More and more evidences show that ctDNA is an excellent tumor biomarker for diagnosis, prognosis, tumor heterogeneity and so on. ctDNA is a tumor code in the blood. Liquid biopsy of ctDNA is firstly summarized. Compared with the traditional detection technologies of ctDNA, the biosensor is an excellent choice for the detection of ctDNA because of its portability, sensitivity, specificity and ease of use. This review mainly evaluates various biosensors applied to the detection of ctDNA. We discuss the most commonly used bioreceptors to specifically identify and bind ctDNA, including complementary DNA (cDNA), peptide nucleic acid (PNA) and anti-5 MethylCytosines, and the biotransducers which convert biological signals to analysable signs. The review also discusses signal amplification strategies in biosensors to detect ctDNA.
语种：
英文
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Antagonistic effect of vitamin E on nAl2O3-induced exacerbation of Th2 and Th17-mediated allergic asthma via oxidative stress
作者：
Cui, Haiyan;Huang, Jiawei;Lu, Manman;Zhang, Qian;Qin, Wei;...
期刊：
Environmental Pollution ,2019年252(Pt B):1519-1531 ISSN：0269-7491
通讯作者：
Li, Rui
作者机构：
[Lu, Manman; Li, Rui; Zhang, Qian; Zhao, Yun; Huang, Jiawei; Lu, Xianxian; Cui, Haiyan; Qin, Wei] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Jiting] Cent China Normal Univ, Inst Nanosci & Nanotechnol, Coll Phys Sci & Technol, Wuhan 430079, Hubei, Peoples R China.;[Xi, Zhuge] Tianjin Inst Environm & Operat Med, 1 Dali Rd, Tianjin 300050, Peoples R China.
通讯机构：
[Li, Rui] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
Aluminum oxide nanopowder;Allergic asthma;Oxidative stress;Th2/Th17;Vitamin E
摘要：
Some basic research has shown that nanomaterials can aggravate allergic asthma. However, its potential mechanism is insufficient. Based on the research that alumina nanopowder (nAl2O3) has been reported to cause lung tissue damage, the purpose of this study was to explore the relationship between nAl2O3 and allergic asthma as well as its molecular mechanism. In this study, Balb/c mice were sensitized with ovalbumin (OVA) to construct the allergic asthma model while intratracheally administered 0.5, 5 or 50 mg kg−1·day−1 nAl2O3 for 3 weeks. It was observed that exposure to nAl2O3 exacerbated airway hyperresponsiveness (AHR), airway remodeling, and inflammation cell infiltration, leading to lung function damage in mice. Results revealed that nAl2O3 could increase ROS levels and decrease GSH levels in lung tissue, promote the increases of the T-IgE, TGF-β, IL-1β and IL-6 levels, stimulate the overexpression of transcription factors GATA-3 and RORγt, decrease the levels of IFN-γ and IL-10 and increase the levels of IL-4 and IL-17A, resulting in the imbalance of Th1/Th2 and Treg/Th17 immune responses. In addition, antioxidant Vitamin E (Vit E) could alleviate asthma-like symptoms through blocking oxidative stress. The study displayed that exposure of nAl2O3 deteriorated allergic asthma through promoting the imbalances of Th1/Th2 and Treg/Th17. Main finding: Intratracheal instillation of nAl2O3 aggravated the imbalance of th2-type th17-type immune, induced airway hyperresponsiveness and subsequently deteriorated allergic asthma in mice. © 2019
语种：
英文
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Rapid Aerobic Inactivation and Facile Removal of Escherichia coli with Amorphous Zero-Valent Iron Microspheres: Indispensable Roles of Reactive Oxygen Species and Iron Corrosion Products.
作者：
Sun, Hongwei;Wang, Jian;Jiang, Yao;Shen, Wenjuan;Jia, Falong* ;...
期刊：
Environmental Science & Technology ,2019年53(7):3707-3717 ISSN：0013-936X
通讯作者：
Jia, Falong;Zhang, Lizhi;Liao, Xiaomei
作者机构：
[Zhang, Lizhi; Sun, Hongwei; Shen, Wenjuan; Jia, Falong; Zhang, LZ] Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Hubei, Peoples R China.;[Wang, Jian; Jiang, Yao; Liao, Xiaomei; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Jia, FL; Zhang, LZ; Liao, Xiaomei] C;Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Zero valent iron (ZVI) is recently regarded as a promising alternative for water disinfection, but still suffers from low efficiency. Herein we demonstrate that amorphous zerovalent iron microspheres (A-mZVI) exhibit both higher inactivation rate and physical removal efficiency for the disinfection of Escherichia coli than conventional crystalline nanoscale ZVI (C-nZVI) under aerobic condition. The enhanced E. coli inactivation performance of A-mZVI was mainly attributed to more reactive oxygen species (ROSs), especially free •OH, generated by the accelerated iron dissolution and molecular oxygen activation in bulk solution. In contrast, C-nZVI preferred to produce surface bound •OH, and its bactericidal ability was thus hampered by the limited physical contact between C-nZVI and E. coli. More importantly, hydrolysis of dissolved iron released from A-mZVI produced plenty of loose FeOOH to wrap E. coli, increasing the dysfunction of E. coli membrane. Meanwhile, this hydrolysis process lowered the stability of E. coli colloid and caused its rapid coagulation and sedimentation, favoring its physical removal. These findings clarify the indispensable roles of ROSs and iron corrosion products during the ZVI disinfection, and also provide a promising disinfection material for water treatment. Copyright © 2019 American Chemical Society.
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英文
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Di-(2-ethylhexyl) phthalate induced an increase in blood pressure via activation of ACE and inhibition of the bradykinin-NO pathway
作者：
Deng, Ting;Xie, Xiaoman;Duan, Jiufei;Chen, Mingqing*
期刊：
Environmental Pollution ,2019年247(Apr.):927-934 ISSN：0269-7491
通讯作者：
Chen, Mingqing
作者机构：
[Chen, Mingqing; Duan, Jiufei; Deng, Ting; Xie, Xiaoman] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chen, Mingqing] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
ACE;Blood pressure;Bradykinin-NO pathway;Di-(2-ethylhexyl) phthalate
摘要：
Epidemiological studies and animal experiments have suggested that exposure to Di-(2-ethylhexyl) phthalate (DEHP) is strongly associated with an increase in blood pressure. However, the mechanisms that result in the detrimental effects of DEHP exposure on blood pressure are unclear. In our study, mice were orally exposed to DEHP dosages of 0.1, 1, 10 mg/kg/day for 6 weeks. The results showed that DEHP could induce a significant increase in systolic blood pressure (SBP) and heart rate, and a significant thickening of the ventricular wall. To explore the underlying mechanism, we measured the level of: angiotensin converting enzyme (ACE); bradykinin B2 receptor (BK2R); endothelial nitric oxide synthase (eNOS); bradykinin and Ca2+ in cardiac cytoplasm as well as in serum nitric oxide (NO). The results suggested that DEHP could induce an increase in ACE levels, and a decrease in bradykinin levels. Moreover, BK2R, Ca2+, eNOS and NO decreased when mice were exposed to 10 mg/kg/day DEHP. Interestingly, 5 mg/kg/day angiotensin converting enzyme inhibitor (ACEI) treatment inhibited the increase in blood pressure, and inhibited the decrease in the levels of BK2R, Ca2+, eNOS, and NO, that were induced by DEHP exposure. Our results suggest that DEHP might increase blood pressure by activating ACE expression, and inhibiting the bradykinin-NO pathway. DEHP exposure activated ACE and inhibited the bradykinin-NO pathway, resulting in an increase in blood pressure. © 2019 Elsevier Ltd
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英文
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Hexavalent chromium removal by a new composite system of dissimilatory iron reduction bacteria Aeromonas hydrophila and nanoscale zero-valent iron
作者：
Shi, Zunji;Shen, Wenjuan;Yang, Kai;Zheng, Ningning;Jiang, Xiaofang;...
期刊：
Chemical Engineering Journal ,2019年362:63-70 ISSN：1385-8947
通讯作者：
Xie, Bo;Ai, Zhihui
作者机构：
[Xie, Bo; Yang, Dandan; Yang, Kai; Shi, Zunji; Jiang, Xiaofang; Zheng, Ningning; Liu, Limin] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Lizhi; Shen, Wenjuan; Ai, Zhihui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Shi, Zunji] Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Xie, Bo; Ai, Zhihui] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Aeromonas hydrophila;Anoxic reduction;Hexavalent chromium;Nanoscale zero-valent iron
摘要：
Core-shell Fe@Fe2O3 nanowire is one special nanoscale zero-valent iron (NZVI) with an iron core and an outside shell of iron oxide containing the surface bound ferrous ions, which has been used to remove various pollutants including Cr(VI). However, it can be easily oxidized to accumulate iron oxides on the shell, resulting in the loss of reactive activity during its long-term application. Therefore, the dissolution of iron oxides by reduction is required to maintain NZVI reactivity. In this study, we combine NZVI and a dissimilatory iron reduction bacteria (DIRB), Aeromonas hydrophila HS01, to establish a composite system for a sustainable anoxic reduction of Cr(VI). HS01 can stably coexist with NZVI, reduce the oxidized Fe on NZVI and generate more dissolved Fe(II) under anoxic conditions. This presence of HS01 can maintain the core-shell structured morphology and relatively more Fe0 and Fe(II) on NZVI surface, and enhance the reactivity in Cr(VI) removal. Our results indicated that a suitable DIRB can be used to prolong the reactivity of NZVI, and their combination is an efficient method for anoxic Cr(VI) removal. © 2019 Elsevier B.V.
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英文
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DINP aggravates autoimmune thyroid disease through activation of the Akt/mTOR pathway and suppression of autophagy in Wistar rats.
作者：
Duan, Jiufei;Deng, Ting;Kang, Jun;Chen, Mingqing*
期刊：
Environmental Pollution ,2019年245(Feb.):316-324 ISSN：0269-7491
通讯作者：
Chen, Mingqing
作者机构：
[Chen, Mingqing; Duan, Jiufei; Deng, Ting; Kang, Jun] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chen, Mingqing] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Autophagy;Di-isononyl phthalate;mTOR;Oxidative stress
摘要：
Di-isononyl phthalate (DINP) is used as a substitute for traditional phthalates, in a wide range of applications. However, there is growing concern regarding its toxicity. Studies have indicated that DINP is related to thyroid hormone disorder and that phthalates can affect thyroid normal function. In this study, we aim to determine any effects of DINP exposure on autoimmune thyroid disease (AITD), the most common autoimmune disease, and to understand the underlying causal mechanism. AITD model Wistar rats were exposed to 0.15 mg/kg, 1.5 mg/kg or 15 mg/kg DINP. We assessed the thyroid globulin antibody levels, Th1/Th2 balance, histopathological changes and caspase-3 levels in the thyroid. The data show that exposure to DINP does indeed aggravate AITD. To explore the underlying mechanisms, we examined the levels of microtubule-associated protein 1 light chain 3 B (LC3B), Sequestosome 1 (SQSTM1) and the appearance of autophagosomes or autolysosomes to assess autophagy in the thyroid. The results show that DINP can suppress normal autophagy. We found that DINP induced an exacerbation of oxidative stress and the activation of the Akt/mTOR pathway, indicating that oxidative stress and activation of mTOR may play a key role in these processes. Moreover, the activation of mTOR also promoted the expression of IL-17. Importantly, blocking oxidative stress with VE or blocking Akt/mTOR with rapamycin mitigated the exacerbation of AITD and the suppression of normal autophagy. All these results indicate that exposure to DINP, especially high doses of DINP, can aggravate oxidative stress and activate the Akt/mTOR pathway. This exposure then leads to a suppression of normal autophagy and expression of IL-17 in the thyroid, resulting in an eventual exacerbation of AITD. In this manuscript, we point out that DINP can induce activation of the Akt/mTOR pathway in the thyroid which activates repression of the normal autophagy process in an aggravation of autoimmune thyroid disease in Wistar rats, which expands our understanding of the adverse effects caused by phthalate exposure, and its impact on AITD, as well as elucidating the underlying mechanism. © 2018 Elsevier Ltd
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英文
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Regional scientific research benefits threatened-species conservation
作者：
Yisi Hu;Zhenhua Luo;Colin A.Chapman;Stuart L.Pimm;Samuel T.Turvey;...
期刊：
国家科学评论(英文) ,2019年6(6):1076-1079 ISSN：2095-5138
通讯作者：
Fan, Pengfei(fanpf@mail.sysu.edu.cn)
作者机构：
[Yisi Hu; Tien Ming Lee; Pengfei Fan] School of Life Sciences, Sun Yat-Sen University, China;[Zhenhua Luo] School of Life Sciences, Central China Normal University, China;Department of Anthropology and School of Environment, McGill University, Canada;[Michael J.Lawes] School of Life Sciences, University of KwaZulu-Natal, South Africa;College of Life Sciences, Northwest University, China
通讯机构：
[Pengfei Fan] S;School of Life Sciences, Sun Yat-Sen University , China
摘要：
Although conventional wisdom considers knowledge of threatened species’ ecology and status essential for conservation, few studies demonstrate this in a quantitative way across many species and within the same political entity. Here, we evaluated the impacts of scientific research against conservation interventions (including funding) and specieslevel correlates, accounting for phylogenetic relatedness, on the conservation of 162 threatened mammal species in China. We did so at three levels: global (all scientific papers published on the species), regional (a subset of the global papers that included at least one author from a local organization) and regional conservation-related (a subset of the regional papers that focused only on ecology and conservation). In addition to protected-area coverage and certain biological traits, regional conservationrelated research emerged as an important predictor of species recovery. The same was not the case for global research. We should particularly encourage future regional research effort that has direct relevance to specific conservation issues.
语种：
英文
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Evaluating the treatment effectiveness of copper-based algaecides on toxic algae Microcystis aeruginosa using single cell-inductively coupled plasma-mass spectrometry
作者：
Shen, Xing;Zhang, Haiting;He, Xiaolong;Shi, Honglan* ;Stephan, Chady;...
期刊：
Analytical and Bioanalytical Chemistry ,2019年411(21):5531-5543 ISSN：1618-2642
通讯作者：
Shi, Honglan;Wan, Cuihong
作者机构：
[Shi, Honglan; He, Xiaolong; Shen, Xing; Zhang, Haiting] Missouri Univ Sci & Technol, Dept Chem, 400 W 11th St, Rolla, MO 65409 USA.;[Wan, Cuihong; Shen, Xing] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Shi, Honglan] Ctr Single Nanoparticle Single Cell & Single Mol, Rolla, MO 65409 USA.;[Stephan, Chady] PerkinElmer Inc, Woodbridge, ON L4L 8H1, Canada.;[Jiang, Hua] City Tulsa, Water & Sewer Dept, Tulsa, OK 74103 USA.
通讯机构：
[Shi, Honglan] M;[Wan, Cuihong; Shi, Honglan] C;Missouri Univ Sci & Technol, Dept Chem, 400 W 11th St, Rolla, MO 65409 USA.;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Ctr Single Nanoparticle Single Cell & Single Mol, Rolla, MO 65409 USA.
关键词：
Copper-based algaecide;Harmful algal bloom;Microcystin-LR;Microcystis aeruginosa;Single cell (SC)-ICP-MS
摘要：
Single cell-inductively coupled plasma-mass spectrometry (SC-ICP-MS) is an emerging technology. In this work, we have developed a novel SC-ICP-MS method to quantify metal ions in individual cells of a toxic cyanobacterial species, Microcystis aeruginosa(M. aeruginosa), without complicated post-dosing sample preparation, and applied this method to study the treatment effectiveness of copper-based algaecides (cupric sulfate and EarthTec®) on the toxic algae M. aeruginosa. The developed SC-ICP-MS method uses new intrinsic metal element magnesium to determine real transport efficiency and cell concentration. The cell viability and microcystin-LR release by algaecide treatment were studied by flow cytometry and ultra-fast liquid chromatography-tandem mass spectrometry, respectively. The results showed that this novel method was very rapid, highly sensitive (detection limits of intracellular copper and magnesium were 65ag/cell and 98ag/cell, respectively), and reproducible (relative standard deviation within 12%). The algaecide effectiveness study further demonstrated that copper in the forms of cupric sulfate and copper-based algaecide EarthTec® successfully diminished M. aeruginosa populations. The higher the copper concentration used to treat the cells, the faster the speeds of copper uptakeand cell lysis in the copper concentrations ranged from 0 to 200μg/L of copper-based algaecide. The cells exhibit obvious heterogeneity in copper uptake. The result suggests that M. aeruginosa cells uptake and cumulate copper followed by cellular lysis and microcystin-LR release. These novel results indicated that though the copper-based algaecides could control this type of harmful algal bloom, further treatment to remove the released algal toxin from the treated water would be needed. [Figure not available: see fulltext.]. © 2019, The Author(s).
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英文
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ɑ-MnO2 nanowire induces cytotoxicity of human lung fibroblasts based on a 3D organotypic culture
作者：
Li, Yan;Li, Jinquan;Wang, Yixin;Wang, Pengcheng;Gong, Zhiyong;...
期刊：
Ferroelectrics ,2019年546(1):1-12 ISSN：0015-0193
通讯作者：
Yang, Wu;Yang, Xu
作者机构：
[Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Li, Jinquan; Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.;[Wang, Yixin; Gong, Zhiyong; Yang, Wu] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Yang, Wu] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Yang, Wu] W;[Yang, Xu] C;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 9th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN)
会议时间：
AUG 17-20, 2018
会议地点：
Hohhot, PEOPLES R CHINA
会议主办单位：
[Li, Yan;Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Li, Jinquan;Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.^[Wang, Yixin;Gong, Zhiyong;Yang, Wu] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.
关键词：
3D organotypic culture;human lung fibroblasts;MnO2 nanowires;nanotoxicity
摘要：
In this study, we performed a 3D organotypic culture of human lung fibroblast (HLF) in a microfluidic device. This 3D HLF model could partly simulate the biological responses of human lung. ɑ-MnO2 nanowires were successfully synthesized using the hydrothermal approach. Material characterization identified the existence of ɑ-MnO2 nanostructure. Based on this 3D organotypic HLF model, our results demonstrated that ɑ-MnO2 nanowires can induce cytotoxicity in dose-dependently. Because the accumulation of reactive oxygen species (ROS) and depletion of glutathione (GSH) are observed in the nanowire-treated cells. In addition, ROS generation induced intracellular increase of malondialdehyde (MDA), DNA damage and activated alpha-smooth muscle actin expression. Based on these results, we concluded that a mechanism for ɑ-MnO2 nanowire-induced pulmary toxicity, which involves the accumulation of ROS, up-regulation of oxidative stress and inflammatory factors. This investigation may provide a fundamental insight to understand the nanotoxicity of wire-shaped nanomaterials. © 2019, © 2019 Taylor & Francis Group, LLC.
语种：
英文
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Stability analysis of nonlinear switched systems with sampled-data controllers
作者：
Wang, Zhichuang;Chen, Guoliang* ;Ba, Hezhen
期刊：
Applied Mathematics and Computation ,2019年357:297-309 ISSN：0096-3003
通讯作者：
Chen, Guoliang
作者机构：
[Wang, Zhichuang] Beijing Inst Technol, Sch Automat, Beijing 100081, Peoples R China.;[Wang, Zhichuang] Beijing Inst Technol, Key Lab Intelligent Control & Decis Complex Syst, Beijing 100081, Peoples R China.;[Chen, Guoliang] Liaocheng Univ, Sch Math Sci, Liaocheng 252000, Shandong, Peoples R China.;[Ba, Hezhen] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chen, Guoliang] L;Liaocheng Univ, Sch Math Sci, Liaocheng 252000, Shandong, Peoples R China.
关键词：
Nonlinear switched systems;Sampled-data;Stability;Emulation
摘要：
This paper addresses the problem of global stability of nonlinear switched systems with sampled-data controllers. By using multiple Lyapunov functions, we obtain sufficient conditions to guarantee nonlinear switched sampled-data systems with arbitrary finite transmission delay are globally exponentially stable. What is more, utilizing the nonlinear emulation techniques, the stability criteria for nonlinear switched sampled-data systems are also derived. Meanwhile, we also present the region of attraction for nonlinear switched sampled-data systems. Finally, some simulation results are presented to verify the validity of our proposed methods. © 2019 Elsevier Inc.
语种：
英文
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Roles of extracellular polymeric substances in the bactericidal effect of nanoscale zero-valent iron: Trade-offs between physical disruption and oxidative damage
作者：
Hu, Ying;Wang, Jian;Sun, Hongwei* ;Wang, Shaohui;Liao, Xiaomei;...
期刊：
Environmental Science: Nano ,2019年6(7):2061-2073 ISSN：2051-8153
通讯作者：
Sun, Hongwei;Wang, Jun
作者机构：
[Hu, Ying; Sun, Hongwei] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Inst Environm Chem,Coll Chem, Wuhan 430079, Hubei, Peoples R China.;[Hu, Ying; Wang, Jun] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Hubei, Peoples R China.;[Wang, Jian; Liao, Xiaomei; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Sun, Hongwei] Pohang Univ Sci & Technol POSTECH, Div Environm Sci & Engn, Pohang 37673, South Korea.;[Wang, Jun] Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China.
通讯机构：
[Sun, Hongwei; Wang, Jun] C;[Sun, Hongwei] P;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Inst Environm Chem,Coll Chem, Wuhan 430079, Hubei, Peoples R China.;Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Hubei, Peoples R China.;Pohang Univ Sci & Technol POSTECH, Div Environm Sci & Engn, Pohang 37673, South Korea.
摘要：
Nanoscale zero-valent iron (nZVI) was known to inactivate various microbes, but the impact of extracellular polymeric substances (EPS) on the bactericidal effect of nZVI remained poorly understood. In this study, we seek to clarify the role of capsular EPS in nZVI inactivation of Escherichia coli under aerobic conditions, by employing parental and isogenic mutants with controlled EPS secretion for comparison. The results indicated that increased EPS of E. coli mitigated the toxicity of nZVI. Surprisingly, more EPS favored the adsorption of nZVI on the E. coli surface, and subsequently resulted in enhanced membrane disruption, which contradicted the E. coli inactivation trends. Nonetheless, E. coli was able to repair the damaged membrane partially with nutrient supply; besides, EPS accelerated the corrosion of nZVI to produce less toxic iron (hydro)oxides. More importantly, EPS could scavenge reactive oxygen species (ROS) produced by oxygenation of nZVI, and prevent ROS from penetrating the cell membrane to damage intracellular structures and macromolecules. Therefore, by increasing EPS secretion, E. coli made trade-offs between physical disruption and oxidative damage to improve the resistance to nZVI inactivation. Besides, similar roles of EPS were also observed in the Gram-positive bacterium Bacillus subtilis. These findings broadened our understanding of the bactericidal mechanism of nZVI, which would benefit the environmental risk assessment of nZVI and the development of ZVI based water disinfection techniques. © 2019 The Royal Society of Chemistry.
语种：
英文
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The effects of PM2.5 on asthmatic and allergic diseases or symptoms in preschool children of six Chinese cities, based on China, Children, Homes and Health (CCHH) project
作者：
Chen, Fei'er;Lin, Zhijing;Chen, Renjie;Norback, Dan;Liu, Cong;...
期刊：
Environmental Pollution ,2018年232(Jan.):329-337 ISSN：0269-7491
通讯作者：
Zhao, Zhuohui
作者机构：
[Liu, Cong; Lin, Zhijing; Kan, Haidong; Zhao, Zhuohui; Chen, Fei'er; Chen, Renjie] Fudan Univ, Sch Publ Hlth, Dept Environm Hlth, Shanghai 200032, Peoples R China.;[Kan, Haidong; Zhao, Zhuohui; Chen, Renjie] Natl Hlth & Family Planning Commiss Peoples Repub, Shanghai Key Lab Meteorol & Hlth, Key Lab Hlth Technol Assessment, Key Lab Publ Hlth Safety,Minist Educ, Shanghai 200032, Peoples R China.;[Wang, Juan; Norback, Dan] Uppsala Univ, Dept Med Sci Occupat & Environm Med, SE-751 Uppsala, Sweden.;[Lu, Chan; Deng, Qihong] Cent South Univ, Sch Energy Sci & Engn, Changsha 410083, Hunan, Peoples R China.;[Huang, Chen; Zou, Zhijun; Liu, Wei; Hu, Yu] Univ Shanghai Sci & Technol, Sch Environm & Architecture, Shanghai 200093, Peoples R China.
通讯机构：
[Zhao, Zhuohui] P;POB 249,130 Dong An Rd, Shanghai 200032, Peoples R China.
关键词：
Air pollution;Asthma;Children;Particulate matter;Hierarchical regression
摘要：
The urbanization and industrialization in China is accompanied by bad air quality, and the prevalence of asthma in Chinese children has been increasing in recent years. To investigate the associations between ambient PM2.5 levels and asthmatic and allergic diseases or symptoms in preschool children in China, we assigned PM2.5 exposure data from the Global Burden of Disease (GBD) project to 205 kindergartens at a spatial resolution of 0.1° × 0.1° in six cities in China (Shanghai, Nanjing, Chongqing, Changsha, Urumqi, and Taiyuan). A hierarchical multiple logistical regression model was applied to analyze the associations between kindergarten-level PM2.5 exposure and individual-level outcomes of asthmatic and allergic symptoms. The individual-level variables, including gender, age, family history of asthma and allergic diseases, breastfeeding, parental smoking, indoor dampness, interior decoration pollution, household annual income, and city-level variable-annual temperature were adjusted. A total of 30,759 children (average age 4.6 years, 51.7% boys) were enrolled in this study. Apart from family history, indoor dampness, and decoration as predominant risk factors, we found that an increase of 10 μg/m3 of the annual PM2.5 was positively associated with the prevalence of allergic rhinitis by an odds ratio (OR) of 1.20 (95% confidence interval [CI] 1.11, 1.29) and diagnosed asthma by OR of 1.10 (95% CI 1.03, 1.18). Those who lived in non-urban (vs. urban) areas were exposed to more severe indoor air pollution arising from biomass combustion and had significantly higher ORs between PM2.5 and allergic rhinitis and current rhinitis. Our study suggested that long-term exposure to PM2.5 might increase the risks of asthmatic and allergic diseases or symptoms in preschool children in China. Compared to those living in urban areas, children living in suburban or rural areas had a higher risk of PM2.5 exposure. Ambient PM2.5 is associated with asthma and allergic diseases and symptoms in Chinese preschool children. © 2017 Elsevier Ltd
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英文
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The NUCLEAR FACTOR-CONSTANS complex antagonizes Polycomb repression to de-repress FLOWERING LOCUS T expression in response to inductive long days in Arabidopsis
作者：
Luo, Xiao;Gao, Zheng;Wang, Yizhong;Chen, Zhijuan;Zhang, Wenju;...
期刊：
PLANT JOURNAL ,2018年95(1):17-29 ISSN：0960-7412
通讯作者：
He, Yuehui
作者机构：
[Wang, Yizhong; Zhang, Wenju; Luo, Xiao; Gao, Zheng; He, Yuehui; Chen, Zhijuan] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China.;[Wang, Yizhong; Zhang, Wenju; Luo, Xiao; Gao, Zheng; He, Yuehui; Chen, Zhijuan] Chinese Acad Sci, Natl Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci, Shanghai 201602, Peoples R China.;[Zhang, Wenju; Gao, Zheng; Chen, Zhijuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Huang, Jirong] Shanghai Normal Univ, Dept Biol, Coll Life & Environm Sci, Shanghai 200234, Peoples R China.;[Yu, Hao] Natl Univ Singapore, Dept Biol Sci, Singapore 117604, Singapore.
通讯机构：
[He, Yuehui] C;Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China.;Chinese Acad Sci, Natl Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci, Shanghai 201602, Peoples R China.
关键词：
Polycomb silencing;Florigen;NF-CO;NF-Y;FLOWERING LOCUS T;photoperiod pathway;PcG proteins;Arabidopsis thaliana
摘要：
Many plants sense the seasonal cues, day length or photoperiod changes, to align the timing of the developmental transition to flowering with changing seasons for reproductive success. Inductive day lengths through the photoperiod pathway induce the expression of FLOWERING LOCUS T (FT) or FT relatives that encode a major mobile florigen to promote flowering. In Arabidopsis thaliana, under inductive long days the photoperiod pathway output CONSTANS (CO) accumulates toward the end of the day, and associates with the B and C subunits of Nuclear Factor Y (NF-Y) to form the NF-CO complex that acts to promote FT expression near dusk, whereas Polycomb group (PcG) proteins function to silence FT expression. How NF-CO acts to antagonize the function of PcG proteins to regulate FT expression remains unclear. Here, we show that the NF-CO complex bound to the proximal FT promoter, through chromatin looping, acts in concert with an NF-Y complex bound to a distal enhancer to reduce the levels of PcG proteins, including both Polycomb repressive complex 1 (PRC1) and PRC2 at the FT promoter, leading to a relieving of Polycomb silencing and thus FT de-repression near dusk. Thus, our study provides molecular insights on how the ‘active’photoperiod pathway and the ‘repressive’Polycomb silencing system interact to control temporal FT expression, conferring the long-day induction of flowering in Arabidopsis.<br/> ©2018 The Authors The Plant Journal ©2018 John Wiley & Sons Ltd
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英文
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