期刊：
Data in Brief,2016年6(1):948-952 ISSN：2352-3409
通讯作者：
Zhang, L.
作者机构：
[Yang, Xu; Zhang, Yuchao; Liu, Xudong; Ding, Shumao] Section of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China;[Zhang, Luoping; McHale, Cliona M] Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, CA, United States
通讯机构：
[Zhang, L.] D;Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, CA, United States
摘要：
Among all phthalate esters, dibutyl phthalate (DBP) is only second to di-(2-ethylhexyl) phthalate (DEHP) in terms of adverse health outcomes, and its potential cerebral neurotoxicity has raised concern in recent years. DBP exposure has been reported to be responsible for neurobehavioral effects and related neurological diseases. In this study, we found that neurobehavioral changes induced by DBP may be mediated by oxidative damage in the mouse brain, and that the co-administration of Mangiferin (MAG, 50 mg/kg/day) may protect the brain against oxidative damage caused by DBP exposure. The results of ethological analysis (elevated plus maze test and open-field test), histopathological examination of the brain, and assessments of oxidative stress (OS) in the mouse brain showed that there is a link between oxidative stress and anxiety-like behavior produced by DBP at higher doses (25 or 125 mg/kg/day). Biomarkers of oxidative stress encompass reactive oxygen species (ROS), glutathione (GSH), malondialdehyde (MDA) and DPC coefficients (DPC). MAG (50 mg/kgiday),administered as an antioxidant,can attenuatetheanxiety-like behavior of the tested mice. (c) 2016 Elsevier By. All rights reserved.
摘要：
Several epidemiological studies have suggested a possible link between exposure to Diisononyl phthalate (DINP) and the development of allergies. These findings remain controversial since there is insufficient scientific evidence to assess the ability of DINP to influence allergic immune responses. In addition, the mechanisms behind DINP-caused allergic diseases have not been fully elucidated. In this study, Balb/c mice were orally exposed to DINP for 3 weeks and were then sensitized with fluorescein isothiocyanate (FITC). We showed that oral exposure to DINP could aggravate allergic-dermatitis-like lesions, indicated by an increase in the number of mast cells, and in increased skin edema in FITC-induced contact hypersensitivity. This deterioration was concomitant with increased total serum immunoglobulin-E and Th2 cytokines. We determined the oxidative damage and the activation of nuclear factor-kb (NF-kB). The data demonstrated that DINP could promote oxidative damage and the activation of NF-kB in the skin. The expression of thymic stromal lymphopoietin and the activation of signal transducer and activator of transcriptions 3, 5 and 6 were enhanced concomitant with exacerbated allergic dermatitis effects and the activation of NF-kB induced by DINP. These effects were alleviated by pyrollidine dithiocarbamate, an inhibitor of NF-kB. The results suggest that oral exposure to DINP aggravated allergic contact dermatitis, which was positively regulated via NF-kB.
作者：
Ye, Ranfeng;Zhu, Chengzhou;Song, Yang;Lu, Qian;Ge, Xiaoxiao;...
期刊：
Small,2016年12(23):3094-3100 ISSN：1613-6810
通讯作者：
Du, Dan;Li, He;Lin, Yuehe
作者机构：
[Ye, Ranfeng; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Zhu, Chengzhou; Lu, Qian; Li, He; Du, Dan; Song, Yang; Lin, Yuehe; Du, D; Lin, YH; Ge, Xiaoxiao] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99163 USA.;[Zhu, Mei-Jun] Washington State Univ, Sch Food Sci, Pullman, WA 99163 USA.;[Li, He] Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Peoples R China.
通讯机构：
[Du, Dan] C;[Du, D; Li, H; Lin, YH] W;[Li, He] U;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
An innovatively bioinspired method has been developed for the green synthesis of multifunctional all-inone organic?inorganic hybrid nanoflowers integrating the functions of biorecognition and signal amplification. The synthesis of Con A-GOx-CaHPO<inf>4</inf>hybrid nanoflowers was carried out in a 1.5 mL eppendorf tube. In a typical synthesis, 0.2769 mg GOx and 0.0231 mg Con A were dissolved in 1 mL buffer solution. The products were purified by three repeated steps with centrifuging at 10 000 rpm for 5 min and then washing with DI water. For SEM observation, the suspension of the prepared nanoflowers was filtered and dried on a membrane and sputter coated with gold. For TEM observation, a drop of the suspension of the prepared nanofl owers was added to a carbon grid and dried at room temperature. Because the molecular weight of Cy5 and FITC is small and could be negligible compared with GOx and Con A, the encapsulation efficiency of GOx-Cy5 and Con A-FITC could be deemed as the encapsulation effi ciency of GOx and Con A, respectively.
作者机构：
[Ye, Ranfeng; Li, He; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Li, He; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Zhu, Chengzhou; Li, He; Lu, Qian; Song, Junhua; Du, Dan; Song, Yang; Fu, Shaofang; Lin, Yuehe; Du, D; Lin, YH] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Zhu, Mei-Jun] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA.
通讯机构：
[Du, Dan] C;[Du, D; Lin, YH] W;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
作者机构：
[Yang X.; Du J.; Zhu Y.; Zou J.; Yu J.; Zhu J.] School of Life Sciences, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Xu Yang] S;School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
H_2O_2;glutathione;oxidative stress;TSLP
摘要：
With increasing industrialization, numerous air pollutants are generated. This research aimed to investigate the effects of inhalation of oxidative pollutants. H2O2 was used to simulate oxidative air pollutants, and glutathione, a reducing agent that is widely distributed in organisms, was used as an antagonist, to protect cells from oxidative stress. H2O2 was diluted using two gradients (0.05 mM, 0.20 mM, 0.80 mM, 3.20 mM and 0.05 mM, 0.10 mM, 0.15 mM, 0.20 mM) and GSH was dissolved at 20 μM. MTT, MDA, ROS, GSH, and TSLP were used as biomarkers to evaluate oxidative stress and possible resulting molecular events. A dose–response relationship was observed between H2O2 concentrations and the above-mentioned biomarkers. Glutathione significantly reduced levels of oxidative stress.
作者机构：
[尤会会; 蔡洁; 杨旭; 李慧; 曹风华; 梅宇飞; 刘志敏] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, China;[丁书茂] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, China. dingsm@mail.ccnu.edu.cn
作者机构：
[Chen, Shaohui; You, Huihui; Yang, Xu; Mao, Lin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Chen, Shaohui] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
摘要：
The prevalence of allergic diseases around the world has been increasing dramatically in recent years. Epidemiological and experimental studies have suggested the involvement of phthalate esters (PAEs) in this increase in allergic diseases. It has been previously reported that PAEs may act as adjuvants, thus contributing to an increase of these diseases. In this study we focus on examining whether dibutyl phthalate (DBP) exhibits an adjuvant effect mediated via an oxidative stress mechanism in a murine asthma model. The DBP was applied through a daily gavage exposure route. Mice were immunized with ovalbumin (OVA) to initiate immune responses, and melatonin (MT) was used as an antioxidant. However, we did not see a significant difference in the level of oxidative stress, which suggested that the possible adjuvant effect of DBP is not via an oxidative stress mechanism. Additionally, the level of immunoglobulin E (IgE) in serum, cytokines and inflammatory cells in bronchoalveolar lavage fluid (BALF) showed no significant difference between the OVA positive control group and the DBP and OVA combined exposure group. The significant difference that was observed in the expiratory resistance of airway hyperresponsiveness (AHR) may be attributed to changes in the three dimensional structure of the airway wall and the slight shrinkage of the airway. The administration of MT significantly reversed all these effects. Taken together with our data, these results suggest that DBP has little or no adjuvant effect in a murine mouse model and is not mediated through an oxidative stress mechanism.
摘要：
Hippocampus-related topographic amnesia is the most common symptom of memory disorders in Alzheimer's disease (AD) patients. Recent studies have revealed that experience-mediated DNA methylation, which is regulated by enzymes with DNA methyltransferase (DNMT) activity, is required for the formation of recent memory as well as the maintenance of remote memory. Notably, overexpression of DNMT3a in the hippocampus can reverse spatial memory deficits in aged mice. However, a decline in global DNA methylation was found in the autopsied hippocampi of patients with AD. Exactly, what endogenous factors that affect DNA methylation still remain to be elucidated. Here, we report a marked increase in endogenous formaldehyde levels is associated with a decline in global DNA methylation in the autopsied hippocampus from AD patients. In vitro and in vivo results show that formaldehyde in excess of normal physiological levels reduced global DNA methylation by interfering DNMTs. Interestingly, intrahippocampal injection of excess formaldehyde before spatial learning in healthy adult rats can mimic the learning difficulty of early stage of AD. Moreover, injection of excess formaldehyde after spatial learning can mimic the loss of remote spatial memory observed in late stage of AD. These findings suggest that aging-associated formaldehyde contributes to topographic amnesia in AD patients. (C) 2015 Elsevier Inc. All rights reserved.
