作者机构：
[Zhang, Jian-Feng; Chu, Hui-Hui; Liao, Dan; Tong, Yi-Kai; Liu, Ying-Ying; Ren, Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Ma, Guang-Jing] Chinese Acad Sci, Innovat Acad Seed Design, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Specialt, Wuhan 430074, Peoples R China.;[Li, Jun] Chinese Acad Agr Sci, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Oil Crops Res Inst, Wuhan, Peoples R China.
通讯机构：
[Feng Ren] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
摘要：
The members of PHOSPHATE 1 (PHO1) family play important roles in plant phosphate (Pi) transport and adaptation to Pi deficiency. The functions of PHO1 family proteins have been reported in several plant species, with the exception of Brassica species. Here, we identified 23, 23, and 44 putative PHO1 family genes in Brassica rapa, Brassica oleracea, and Brassica napus by whole genome analysis, respectively. The phylogenetic analysis divided PHO1 family proteins into eight groups, which represented the orthologous relationships among PHO1 members. The gene structure and the conserved motif analysis indicated that the most PHO1 family genes had similar gene structures and the PHO1 proteins shared mutual conserved motifs. The chromosome distribution analysis showed that the majority of BnPHO1 family genes distributed analogously at chromosomes with BrPHO1 and BoPHO1 family genes. The data showed that PHO1 family genes were highly conserved during evolution from diploid to tetraploid. Furthermore, the expression analysis showed that PHO1 family genes had different expression patterns in plant tissues, suggesting the diversity of gene functions in Brassica species. Meanwhile, the expression analysis also revealed that some PHO1 family genes were significantly responsive to Pi deficiency, suggesting that PHO1 family genes play critical roles in Pi uptake and homeostasis under low Pi stress. Altogether, the characteristics of PHO1 family genes provide a reliable groundwork for further dissecting their functions in Brassica species.
摘要：
Understanding trophic niche differentiation is critical for exploring interspecific competition and stable coexistence among morphologically similar sympatric species. Bats are an ideal model for studying trophic niche differentiation among species because of their high taxonomic and ecological diversities, as well as their special life history traits. Although many factors can affect bat trophic niches, few studies have combined multiple factors to investigate the influences on bat trophic niches. In this study, we analyzed the summer diet and potential influencing factors of five sympatric rhinolophid bats in southwestern China: Rhinolophus macrotis, Rhinolophus osgoodi, Rhinolophus ferrumequinum, Rhinolophus affinis, and Rhinolophus pusillus. All five species mainly fed on Lepidopteran and Dipteran insects, with a large trophic niche breadth for each species and a low degree of dietary overlap. With the exception of R. affinis and R. pusillus, significant differences in diet composition were detected among species, which indicated relatively low interspecific competition. Canonical correspondence analysis showed that both echolocation calls and body size significantly affected interspecific diet differentiation, while wing morphology and bite force had relatively weak effects. This study suggests that the combined effects of multiple factors may drive trophic niche differentiation among five rhinolophid bat species in the study area.
通讯机构：
[Zeng, Y.] B;[Li, J.] J;Brain Science and Advanced Technology Institute, School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China
作者机构：
[Luo, Jingwen; Chen, Feng; Xu, Wenliang; Li, Juan; Guo, Yanjun; Qiao, Mengfei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zeng, Wei] Zhejiang A&F Univ, Coll Forestry & Biotechnol, Sino Australia Plant Cell Wall Res Ctr, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China.
通讯机构：
[Xu, Wenliang] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Cellulose synthesis;Cotton fiber;DUF288 domain containing proteins;Fiber development
摘要：
Cotton is one of the most important economic crops in the world, with over 90 % cellulose in the mature fiber. However, the cellulose synthesis mechanism in cotton fibers is poorly understood. Here, we identified four DUF288 domain containing proteins, which we designated GhSTL1-4. These four GhSTL genes are highly expressed in 6 days post anthesis (dpa) and 20 dpa cotton fibers. They are localized to the Golgi apparatus, and can rescue the growth defects in primary cell wall (PCW) and secondary cell wall (SCW) of cellulose synthesis of the Arabidopsis stl1stl2 double mutant at varying degrees. Silencing of GhSTLs resulted in reduced cellulose content and shorter fibers. In addition, split-ubiquitin membrane yeast two-hybrid analysis showed that GhSTL1 and GhSTL4 can interact with PCW-related GhCesA6-1/6-3 and SCW-associated GhCesA7-1/7-2. GhSTL3 can interact with SCW-related GhCesA4-3. These interactions are further confirmed by firefly luciferase complementation imaging assay. Together, we demonstrate that GhSTLs can selectively interact with both the PCW and SCW-associated GhCesAs and impact on cellulose synthesis and fiber development. Our findings provide insights into the mechanism underlying cellulose biosynthesis in cotton fibers, and offer potential candidate genes to coordinate PCW and SCW cellulose synthesis of cotton fibers for developing elite cotton varieties with enhanced fiber quality.
作者机构：
[Wu, Wei; Wang, Meng; Wei, Fuwen; Hu, Yibo; Yang, Lin; Zhan, Xiangjiang; Hou, Xian; Fan, Huizhong] Chinese Acad Sci, Inst Zool, CAS Key Lab Anim Ecol & Conservat Biol, Beijing, Peoples R China.;[Wu, Wei; Zhang, Wenyan; Wang, Meng; Wei, Fuwen; Hu, Yibo; Yang, Lin; Hou, Xian] Univ Chinese Acad Sci, Beijing, Peoples R China.;[Wei, Fuwen; Hu, Yibo; Zhan, Xiangjiang] Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China.;[Chen, Youhua; Zhang, Wenyan] Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Peoples R China.;[Chang, Jiang] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing, Peoples R China.
通讯机构：
[Wei, Fuwen] C;[Wei, Fuwen] U;[Wei, Fuwen] S;Chinese Acad Sci, Inst Zool, CAS Key Lab Anim Ecol & Conservat Biol, Beijing, Peoples R China.;Univ Chinese Acad Sci, Beijing, Peoples R China.
摘要：
Genetic diversity and phylogenetic diversity reflect the evolutionary potential and history of species, respectively. However, the levels and spatial patterns of genetic and phylogenetic diversity of wildlife at the regional scale have largely remained unclear. Here, we performed meta-analyses of genetic diversity in Chinese terrestrial vertebrates based on three genetic markers and investigated their phylogenetic diversity based on a dated phylogenetic tree of 2461 species. We detected strong positive spatial correlations among mitochondrial DNA-based genetic diversity, phylogenetic diversity, and species richness. Moreover, the terrestrial vertebrates harbored higher genetic and phylogenetic diversity in South China and Southwest China than in other regions. Last, climatic factors (precipitation and temperature) had significant positive effects while altitude and human population density had significant negative impacts on levels of mitochondrial DNA-based genetic diversity in most cases. Our findings will help guide national-level genetic diversity conservation plans and a post-2020 biodiversity conservation framework.
期刊：
FRONTIERS IN PLANT SCIENCE,2021年12:767667 ISSN：1664-462X
通讯作者：
Huang, G.;Liu, L.
作者机构：
[Hu, Qianqian; Zeng, Mengting; Huang, Xiaoyu; Wang, Miao] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China;[Li, Jiayi; Xuan, Lijie; Liu, Lu] Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China;[Feng, Changhui] Institute of Cash Crops, Hubei Academy of Agricultural Sciences, Wuhan, China;Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Ürümqi, China;[Huang, Gengqing] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Ürümqi, China
通讯机构：
[Huang, G.] H;[Liu, L.] J;Joint Center for Single Cell Biology, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, China
关键词：
Cotton;main stem apex development;GhMCTP;Gene Expression;Protein interaction;KNOX family protein 3
作者机构：
[Zheng, Yong; Guo, Jilin; Wang, Hongbin; Tian, Zhongping; Li, Jin] Xinjiang Normal Univ, Xinjiang Key Lab Special Species Conservat & Regu, Coll Life Sci, Urumqi 830054, Peoples R China.;[Yuan, Yongze; Zheng, Yong; Zheng, Y; Deng, Lingfu] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Zheng, Yong] X;[Zheng, Y; Yuan, YZ] C;Xinjiang Normal Univ, Xinjiang Key Lab Special Species Conservat & Regu, Coll Life Sci, Urumqi 830054, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
摘要：
The traditional Chinese desert shrub Lycium ruthenicum is widely distributed in arid environments such as north-west China, exhibiting ideal salt tolerance to cope with soil desertification, salinity, and alkalinity. However, the salt-tolerance mechanism of L. ruthenicum, especially of its young seedlings at early vegetative stages, remains largely unknown. In the present study, we collected whole-seedling samples from Lycium ruthenicum at a-pair-leaf stage with and without a mild salt (75 mM sodium chloride) treatment, and then performed transcriptome profiling to compare their gene expression patterns. The de novo assembly achieved 94,651 unigenes with 55,156 annotated. Among them, 199 differentially expressed genes (DEGs) were identified between salt-treated seedlings and control, with 41 up-regulated and 158 down-regulated. These DEGs were highly enriched into gene ontology (GO) classifications 'metabolic process' and 'catalytic activity', into Clusters of Orthologous Groups (COG) function classifications 'translation, ribosomal structure and biogenesis' and 'energy production and conversion', and into Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways 'ribosome' and 'oxidative phosphorylation'. Specifically, genes involved in energy metabolism (oxidative phosphorylation) and related energy-consuming metabolisms, including ribosome-associated biogenesis and biosynthesis of organic nitrogen-derived compatible solutes (i.e., arginine and proline), were generally down-regulated. Specific genes involved in abscisic acid (ABA) biosynthesis and signaling pathway were simultaneously up-regulated. Changes in the transcript levels of salt-responsive DEGs selected from the transcriptomic profiling were further validated by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Based on these results, salinity-adaptive strategies for the L. ruthenicum early seedlings are discussed.
作者机构：
[Feng CHEN; Yanjun GUO; Li CHEN; Xinli GAN; Min LIU; Juan LI] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China;Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China;[Wenliang XU] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China<&wdkj&>Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China
通讯机构：
[Wenliang XU] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China<&wdkj&>Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China
摘要：
Mature cotton fiber secondary cell wall comprises largely of cellulose (> 90%) and small amounts of xylan and lignin. Little is known about the cotton fiber xylan biosynthesis by far. To comprehensively survey xylan biosynthetic genes in cotton fiber, we identified five IRX9, five IRX10, one IRX14, six IRX15, two FRA8, one PARVUS, eight GUX, four GXM, two RWA, two AXY9, 13 TBL genes by using phylogenetic analysis coupled with expression profile analysis and co-expression analyses. In addition, we also identified two GT61 members, two GT47 members, and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized. These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis. Further complementation analysis indicated that one IRX10 like and two FRA8 related genes were able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in their respective Arabidopsis mutant. We conclude that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis. The list of 55 cotton genes presented here provides not only a solid basis to uncover the biosynthesis of xylan in cotton fiber, but also a genetic resource potentially useful for future studies aiming at fiber improvement via biotechnological approaches.
摘要：
Sex-biased dispersal, which influences species' social organization, genetic structure and evolution, is an important life-history event. Although sex-biased dispersal in mammals and birds has been thoroughly studied, little is known about it in amphibians. In this study, we used eight highly polymorphic microsatellite loci and two mitochondrial DNA (mtDNA) genes to infer patterns of dispersal of the Omei tree frog (Rhacophorus omeimontis). We found that the comparison between two types of markers showed higher F-ST values in mtDNA compared to microsatellites. Consistently, comparison of pairwise and overall F-ST values between the sexes revealed higher differentiation in females than in males. Furthermore, the inbreeding coefficient (F-IS) and population gene diversity (H-S) were lower in females than in males, whereas relatedness (r) was higher in females than in males. Females exhibited relatively higher correlation between genetic distance and geographical distance than males. Additionally, the mean assignment index (mAI(C)) was lower in males, whereas the variance of assignment index (vAI(C)) was higher in males. These evidences reflect higher genetic differentiation in females than in males, indicating male-biased dispersal in the Omei tree frog. Considering the life history reported in this species, we discussed potential mechanisms driving the male-biased dispersal in the Omei tree frog.
摘要：
NAC domain transcription factors (TFs) are plant-specific transcriptional regulators, some of which play crucial roles in secondary cell wall (SCW) biosynthesis in plants. Cotton is one of the most important natural fiber producing crops, whose mature fiber SCW contains more than 90% cellulose with very small amounts of xylan and lignin, but little is known about the molecular mechanism underlying fiber SCW formation. We previously identified seven fiber preferentially expressed NAC members, GhFSN1-7. One, GhFSN1, was demonstrated to positively regulate fiber SCW thickening, but the functions of other GhFSN members remain unknown. In this study, roles of GhFSN5 were dissected. qRT-PCR analysis showed that GhFSN5 was predominantly transcribed during the fiber SCW thickening stage. In addition, a large number of fiber SCW biosynthetic genes and SCW-related TFs were co-expressed with GhFSN5. Heterologous expression of GhFSN5 in Arabidopsis resulted in plants with smaller siliques and severe sterility. Anther dehiscence in transgenic lines was not substantially affected, but most pollen was collapsed and nonviable. Furthermore, cellulose and lignin contents in inflorescence stems as well as roots were reduced in transgenic lines, compared with the wild type. Moreover, a set of SCW biosynthetic genes for cellulose, xylan and lignin and several transcription factors involved in regulation of SCW formation were down-regulated in transgenic plants. Our findings indicate that GhFSN5 acts as a negative regulator of SCW formation and anther development and expands our understanding of transcriptional regulation of SCW biosynthesis.
摘要：
Around the globe, worsening air pollution is spawning major public health and environmental concerns, especially in the poorest and most populous cities. As a major secondary air pollutant, ozone is a potential risk factor for exacerbated asthma, although the underlying mechanisms remain uncertain. In this study, we aim to investigate the role of ozone on asthma exacerbation using a classic asthmatic model with allergic airway inflammation by treating Balb/c mice with ovalbumin (OVA). Our study shows ozone exposure significantly exacerbated OVA-induced asthmatic phenotypes, including serum immunoglobulin, Th cytokines, inflammatory cell counts, mucus production, airway remodeling, and airway hyper responsiveness (AHR). Interestingly, expression of transient receptor potential cation channel subfamily V memberl (TRPV1) was also significantly elevated in ozone-exacerbated asthmatic mice and that treatment with TRPVI antagonist effectively suppressed AHR, airway inflammation and remodeling. The underlying mechanisms of these effects may be associated with suppression of neuropeptide calcitonin gene-related peptide (CGRP) and thymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine. Base on the role of TRPV1 in allergic asthma, this study further revealed that inhibition of TRPVI by TRPV1 antagonist has significant anti-inflammatory effects on ozone-induced asthma exacerbation in this study. Induction of TRPVI expression may be an important mechanism underlying the increased risks for asthma after exposure to environmental pollutants. (C) 2019 Elsevier Ltd. All rights reserved.
