期刊：
Proceedings of the National Academy of Sciences of the United States of America,2022年119(32):e2209904119 ISSN：0027-8424
通讯作者：
Stang, P.J.;Sun, Y.
作者机构：
[Xu, Yuling; Ma, Xin; Sun, Yao; Li, Chonglu] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Key Lab Pesticides & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;[Stang, Peter J.; Tuo, Wei; Sun, Yan] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA.;[Tu, Le] Hubei Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Peoples R China.;[Li, Xiaopeng] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Guangdong, Peoples R China.;[Sun, Yan] Henan Univ, Minist Educ, Key Lab Special Funct Mat, Kaifeng 475004, Peoples R China.
通讯机构：
[Yuling Xu; Chonglu Li; Xin Ma; Yao Sun] K;[Yan Sun; Peter J. Stang] D;Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China<&wdkj&>Department of Chemistry, University of Utah, Salt Lake City, UT 84112<&wdkj&>Ministry of Education Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, China<&wdkj&>Department of Chemistry, University of Utah, Salt Lake City, UT 84112
作者机构：
[Wang, Li; Li, Haibing; Feng, Fudan; Cheng, Jing; Zhu, Fei] Cent China Normal Univ CCNU, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Zhou, Juan] Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, R China, Wuhan 430071, Peoples R China.;[Zhu, Fei] Hubei Univ Med, Sch Basic Med Sci, Dept Pharmacol, Hubei Key Lab Wudang Local Chinese Med Res, Shiyan 442000, Hubei, Peoples R China.;[Trautmann, Christina; Toimil-Molares, Maria Eugenia] GSI Helmholtzzentrum Schwerionenforsch, D-64291 Darmstadt, Germany.;[Trautmann, Christina] Tech Univ Darmstadt, D-64287 Darmstadt, Germany.
通讯机构：
[Juan Zhou] S;[Jing Cheng] K;State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, P. R. China<&wdkj&>Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University (CCNU), Wuhan 430079, P. R. China
作者机构：
[Yang, Xiaojie; Leng, Xiebin; Zhong, Hongying; Qi, Yinghua; Jiang, Ruowei; Tan, Ying; Zhang, Wenyang; Yang, Xiaoyu; Hu, Xuewen] Cent China Normal Univ, Lab Mass Spectrometry, Coll Chem, Key Lab Pesticides & Chem Biol,Minist Educ, Wuhan 430079, Hubei, Peoples R China.;[Zhang, Wenyang] Guangdong Acad Agr Sci, Guangdong Key Lab Crop Germplasm Resources Preven, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China.;[Zhong, Hongying] Guangxi Univ, Ctr Instrumental Anal, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China.
通讯机构：
[Hongying Zhong] L;Laboratory of Mass Spectrometry, College of Chemistry, Central China Normal University, Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, Wuhan, Hubei 430079, P. R. China<&wdkj&>Center for Instrumental Analysis, College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 530004, P. R. China
作者机构：
[Feng, Guoqiang; Hong, Jiaxin; Li, Qianhua; Gong, Shengyi; Feng, Shumin] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
通讯机构：
[Jing Cheng; Haibing Li] S;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China<&wdkj&>Key Laboratory of Pesticide and Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China
作者机构：
[Cheng, Ying; Rao, Li; Xiao, Wen-Jing; Chen, Jia-Rong; Wu, Ya-Li] Cent China Normal Univ, Coll Chem, Minist Educ, CCNU uOttawa Joint Res Ctr,Key Lab Pesticides & Ch, Wuhan 430079, Peoples R China.;[Jiang, Min] Hangzhou Normal Univ, Coll Mat Chem & Chem Engn, Hangzhou 310036, Peoples R China.;[Chen, Jia-Rong] Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China.
通讯机构：
[Jia-Rong Chen] C;CCNU-uOttawa Joint Research Centre, Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China<&wdkj&>School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, P. R. China
摘要：
A three-component 1,2-aminooxygenation reaction of 1,3-dienes by dual photoredox and copper catalysis is described. This protocol uses N-aminopyridinium salts as N-centered radical precursors and nucleophilic alcohols as oxygen sources, providing modular and practical access to 1,2-aminoalkoxylation products with good yields and regioselectivity. Preliminary mechanistic studies support the radical property of the reaction and the involvement of N-centered radical intermediates.
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2022年70(33):10134-10143 ISSN：0021-8561
通讯作者：
Lingling Feng<&wdkj&>Haifeng Qian
作者机构：
[Qian, Haifeng; Feng, Lan; Ke, Mingjing; Lu, Tao; Hu, Hang; Yang, Yaohui; Yu, Zhitao] Zhejiang Univ Technol, Coll Environm, Hangzhou 310014, Peoples R China.;[Huang, Shi; Feng, Lingling] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol CCNU, Wuhan 430079, Peoples R China.;[Huang, Shi; Feng, Lingling] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;[Peijnenburg, Willie J. G. M.] Leiden Univ, Inst Environm Sci CML, NL-2300 RA Leiden, Netherlands.;[Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm RIVM, Ctr Safety Subst & Prod, NL-3720 Bilthoven, Netherlands.
通讯机构：
[Lingling Feng] K;[Haifeng Qian] C;Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education;College of Chemistry, Central China Normal University, Wuhan430079, China<&wdkj&>College of Environment, Zhejiang University of Technology, Hangzhou310014, China
摘要：
Harmful cyanobacterial blooms (HCBs) caused by Microcystis aeruginosa are of great concern as they negatively affect the aquatic environment and human health. Chemical methods could rapidly eradicate HCBs and have been used for many decades. However, many chemical reagents are not recommended to eliminate HCBs in the long term, given the possible destructive and toxic effects of the chemicals employed on non-target aquatic organisms. We developed a new algaecide, 2-((1,3,4-thiadiazol-2-yl)thio)-N-(4-chlorophenyl) acetamide (Q2), to control harmful cyanobacteria while being environmentally friendly and selective. In our study, Q2 effectively inhibited cyanobacterial growth, especially of M. aeruginosa, but did not affect eukaryotic algae in test concentrations. A critical mechanism was revealed by transcriptome and metagenomic results showing that Q2 affects multiple cellular targets of cyanobacteria for HCB control, including the destruction of organelles, damage in the photosynthesis center, as well as inhibition of gas vesicle growth, and these changes can be highly relevant to the decrease of quorum-sensing functional KEGG pathways. Furthermore, Q2 did not affect the microbial composition and could recover the disrupted aquatic functional pathways in a short period. This is different from the impact on ecosystem functioning of the traditionally used harmful algaecide diuron. All these results verified that Q2 could be friendly to the aquatic environment, providing a new directional choice in managing HCBs in the future.
期刊：
Journal of Hazardous Materials,2022年432:128707 ISSN：0304-3894
通讯作者：
Chengzhou Zhu
作者机构：
[Xu, Weiqing; Huang, Jiajia; Zhu, Chengzhou; Sha, Meng; Jiao, Lei; Gu, Wenling; Wang, Hengjia; Wu, Zhichao] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;[Hu, Liuyong] Wuhan Inst Technol, Sch Mat Sci & Engn, Wuhan 430205, Peoples R China.
通讯机构：
[Chengzhou Zhu] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
关键词：
*Biosensing;*Enzyme immobilizations;*Metal-organic frameworks;*Noble metal hydrogels;*Organophosphorus pesticides
摘要：
Cell-free enzymatic catalysis (CFEC) is emerging biotechnology that simulates biological transformations without living cells. However, the high cost of separation and preparation of the enzyme has hindered the practical application of the CFEC. Enzyme immobilization technologies using solid supports to stabilize enzymes have been regarded as an efficient strategy to address this issue. Nevertheless, the activity and stability of the immobilized enzymes are still crucial challenges for working in vitro. Herein, an enzyme immobilization platform is developed by using PtCu hydrogels coated with amorphous metallic-organic frameworks (MOFs) as multifunctional carriers to encapsulate horseradish peroxidase (HRP). Specifically, PtCu hydrogels acting as a "reservoir of metal ions" can interact with the immobilized enzyme and facilitate electron transfer, leading to the boosted enzyme catalytic performances. Furthermore, amorphous MOFs on the surface of PtCu hydrogels serve as an "armor" to protect the internal enzymes from various perturbation environments. The resultant enzyme immobilization platform (PtCu@HRP@ZIF-8) not only shows an approximately 2.4-fold enhanced activity compared with free enzyme but also exhibits improved stability against harsh conditions. The PtCu@HRP@ZIF-8-based biosensor is constructed for sensitive sensing of organophosphorus pesticides (OPs). The proposed biosensor exhibits a favorable linear relationship with the concentration of paraoxon-ethyl from 6 to 800 ng/mL, with a low detection limit of 1.8 ng/mL. This work reveals the promising potential of our proposed enzyme immobilization platform in practical applications.
期刊：
Analytical and Bioanalytical Chemistry,2022年414(17):4809-4819 ISSN：1618-2642
通讯作者：
Zhong, Hongying(hyzhong@gxu.edu.cn)
作者机构：
[Shen, Baojie; Yang, Xiaojie; Zheng, Shi; Zhong, Hongying; Tan, Ying; Liu, Yanping; Jia, Shanshan] Cent China Normal Univ, Minist Educ, Key Lab Pesticides & Chem Biol, Lab Mass Spectrometry,Coll Chem, Wuhan 430079, Hubei, Peoples R China.;[Zhong, Hongying] Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agro B, Ctr Instrumental Anal, Nanning 530004, Guangxi, Peoples R China.
通讯机构：
[Hongying Zhong] L;Laboratory of Mass Spectrometry, College of Chemistry, Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan, People’s Republic of China<&wdkj&>Center for Instrumental Analysis, State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, College of Life Science and Technology, Guangxi University, Nanning, People’s Republic of China
摘要：
Quintrione is a new post-emergence herbicide developed for use in rice; however, the mechanism of action remains unclear. We determined the phytotoxicity of quintrione, and the contributions of hormone levels and lipid peroxidation to phytotoxicity, by comparing them to those induced by quinclorac. We also investigated 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and carotenoid content following treatment with quintrione by comparing them to those induced by quinclorac and mesotrione. We found that quintrione and quinclorac both inhibited the growth of Echinochloa crusgalli var. zelayensis, but that quinclorac was a little more effective. At 24 h, quintrione and quinclorac significantly increased ethylene production and the contents of abscisic acid (ABA) and indole acetic acid (IAA) compared with the control. No significant differences were observed between quintrione and quinclorac on the three plant hormones. Quintrione and quinclorac also induced the formation of malondialdehyde (MDA), which is associated with lipid peroxidation, with no significant difference between them. Carotenoid content was reduced in E. crusgalli var. zelayensis following treatments with quintrione, quinclorac, and mesotrione. At 120 h, carotenoid contents were significantly higher following the quintrione and quinclorac treatments, in comparison with mesotrione treatment. There were no significant differences between quintrione and quinclorac in the inhibition of HPPD activity, and the effects of both were significantly less than the effect of mesotrione. In summary, E. crusgalli var. zelayensis was susceptible to both quintrione and quinclorac. The mechanism of action of quintrione, like that of quinclorac, was related to levels of plant hormones and lipid peroxidation; however, quintrione was a poor inhibitor of HPPD activity compared to mesotrione.
期刊：
Journal of Chromatography A,2022年1676:463258 ISSN：0021-9673
通讯作者：
Hui Xu
作者机构：
[Liu, Ying; Tian, Xinmeng; Xu, Hui] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Zhang, Ganbing] Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Peoples R China.
通讯机构：
[Hui Xu] K;Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, China
摘要：
Nowadays, environment fate and behavior of pesticides in soil is still not fully understood due to the lack of standardized soil extraction method. In this work, a soil-filled micro-matrix cartridge was online combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) through a six-way valve for the simultaneous extraction and determination of residual fipronil in soil. Compared with conventional extraction methods, such as hydroxypropyl-beta-cyclodextrin (HPCD) extraction, shaking extraction, ultrasonic-assisted extraction (UAE), three-step extraction and matrix solid phase dispersion (MSPD), the novel, miniaturized, and integrated online micro-matrix cartridge extraction (online mu-MCE) method exhibited better performance in terms of desorption efficiency (99.4%), analysis time, solvent consumption, sensitivity, and automation. In sequential extraction, online mu-MCE could further desorb fipronil from the extracted soil with the percentage of 1.05%-58.55%. High recovery of 92.69% obtained for the ISO certificated test-soil verified the satisfactory accuracy of the method. Besides, its wide universality was also validated in three variables: 1) various pesticides-soil interactions, 2) four types of compounds (aromatic hydrocarbons, carboxylic acids, alcohols and aldehydes), and 3) three types of soils (sandy soil, silty loam and silty clay). The superior desorption capacity might be attributed to the instantaneously increased high-pressure, continuous flow dynamic desorption and short residence time. The present encouraging findings might shed light on new ways to develop a mild, highly efficient, reliable and one-fit-all extraction method toward pesticide contaminated soil. (C) 2022 Elsevier B.V. All rights reserved.
作者机构：
[Xu, Hao; Qian, Wei-Feng; He, Jin-Yu; Zhu, Cuiju; Zhong, Bing] Cent China Normal Univ, CCNU uOttawa Joint Res Ctr, Key Lab Pesticides & Chem Biol, Minist Educ,Coll Chem,Int Joint Res Ctr Intellige, Wuhan 430079, Peoples R China.
通讯机构：
[Cuiju Zhu; Hao Xu] C;CCNU-uOttawa Joint Research Centre, Key Laboratory of Pesticides & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
关键词：
Bioactive compounds;C(sp(3))−H oxygenation;Electrochemical;Phenols;Water as oxygen source
摘要：
Electrochemical C(sp3) -H oxygenation of phenols derivatives were accomplished using H2O as the sole oxygen source. Thus, selectively oxidative 4-cresols to 4-acylphenols derivatives proved viable with the aid of indirect electrolysis, through metal-and external oxidant free environmental-friendly and safe electrocatalytic condi-tions. Advances of this strategy were proved by its ability to transform various sulfonamides with excellent site and regioselectivity. Detailed mechanistic studies allowed to delineate the exact profile of the generation of the oxygenation events. <comment>Superscript/Subscript Available</comment
期刊：
International Journal of Molecular Sciences,2022年23(20):12673- ISSN：1422-0067
通讯作者：
Changlin Liu
作者机构：
[Wang, Mingfang; Cui, Yanfang; Liu, Chunrong; Wang, Huiling; Huang, Xiaoping; Liu, Changlin; Li, Xiang; Qiu, Shuang; Nie, Zefeng] Cent China Normal Univ, Sch Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Changlin Liu] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, School of Chemistry, Central China Normal University, Wuhan 430079, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Cu/Zn superoxide dismutase (SOD1) plays a key role in the maintenance of cellular reactive oxygen species (ROS) homeostasis as an antioxidant enzyme. We recently found that SOD1 is involved in the regulation of gene expression in response to changes in cellular ROS levels by binding to DNA-specific sequences. Moreover, the SOD1 binding to DNA was observed to be redox-dependent in solutions. Thus, we examined the redox-dependent DNA binding of SOD1 by multiple measurements, including small-angle X-ray scattering (SAXS), indicating the redox-dependent formation of a DNA-SOD1 complex in solutions. The redox-dependent formation of the DNA-SOD1 complex could underlie the SOD1 regulation of gene expression.
作者：
Zhu, Yan-Ping;Zhou, Yu;Li, Wen-Juan;Liu, Fu-Rao;Wang, R. China Wen-Cheng;...
期刊：
JOURNAL OF ORGANIC CHEMISTRY,2022年87(18):12460-12469 ISSN：0022-3263
通讯作者：
Yan-Ping Zhu
作者机构：
[Liu, Fu-Rao; Li, Wen-Juan; Zhou, Yu; Chao, Bing-Yu; Hao, Kai-Yan; Wang, R. China Wen-Cheng; Sun, Yun-Yuan; Zhu, Yan-Ping; Shi, Tian-Ru] Yantai Univ, Key Lab Mol Pharmacol & Drug Evaluat, Collaborat Innovat Ctr Adv Drug Delivery Syst & B, Sch Pharm,Minist Educ, Yantai 264005, Shandong, Peoples R China.;[Wu, An-Xin] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Yan-Ping Zhu] S;School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Shandong Yantai, 264005, P. R. China
摘要：
A high efficiency protocol was developed for the synthesis of 2,5-disubstituted oxazoles via iodine-promoted oxidative domino cyclization. These reactions were performed with readily available methyl azaarenes and α-amino ketones under metal-free conditions. This protocol is a simple method with high functional group compatibility, a wide range of substrates, and excellent yield, providing a new way to synthesize azaarene-attached oxazoles.
作者机构：
[Dong, Xiongwei; Wang, Huipeng; Wang, Qian; Li, Xiang] Wuhan Text Univ, Natl Local Joint Engn Lab Adv Text Proc & Clean Pr, Wuhan 430073, Hubei, Peoples R China.;[Li, Xiang] Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Hubei, Peoples R China.;[Li, Xiang] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China.;[Zhang, Hui] Wuhan Text Univ, Sch Fash, Wuhan 430073, Hubei, Peoples R China.;[Li, Man] Cent China Normal Univ, Sch Chem, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wang, Q.] N;[Li, X.] C;National Local Joint Engineering Laboratory for Advanced Textile Processing and Clean Production, Hubei, China;College of Chemistry and Chemical Engineering, Hubei, China
